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Article: Use of capillary electrophoresis-based competitive immunoassay for a large molecule

TitleUse of capillary electrophoresis-based competitive immunoassay for a large molecule
Authors
Issue Date1999
Citation
Journal Of Chromatography B: Biomedical Sciences And Applications, 1999, v. 727 n. 1-2, p. 63-71 How to Cite?
AbstractA systematic study on the optimization of capillary electrophoresis-based immunoassay (CEIA) was performed using bovine serum albumin (BSA) and monoclonal anti-BSA. The immunocomplex could not be resolved from free BSA or anti-BSA with UV detection. When fluorescein isothiocyanate-labeled BSA (FITC-BSA) was used as tracer, the free and bound FITC-BSA were well separated giving definite peaks with laser induced fluorescence detection. The factors affecting the separation of the free and bound FITC-BSA, including voltage, pH and ionic strength of the running buffer, were systematically analyzed. Competitive CEIAs were demonstrated in uncoated and coated capillaries with whole or Fab fragment of the antibody. The coefficient of variation for the quantification of BSA in coated capillary was less than that in uncoated capillary. This study demonstrated that competitive CEIA could be applied to quantify high-molecular-mass protein in biological fluids. Copyright (C) 1999 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/173231
ISSN
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorOu, JPen_US
dc.contributor.authorWang, QGen_US
dc.contributor.authorCheung, TMen_US
dc.contributor.authorChan, STHen_US
dc.contributor.authorYeung, WSBen_US
dc.date.accessioned2012-10-30T06:28:39Z-
dc.date.available2012-10-30T06:28:39Z-
dc.date.issued1999en_US
dc.identifier.citationJournal Of Chromatography B: Biomedical Sciences And Applications, 1999, v. 727 n. 1-2, p. 63-71en_US
dc.identifier.issn1387-2273en_US
dc.identifier.urihttp://hdl.handle.net/10722/173231-
dc.description.abstractA systematic study on the optimization of capillary electrophoresis-based immunoassay (CEIA) was performed using bovine serum albumin (BSA) and monoclonal anti-BSA. The immunocomplex could not be resolved from free BSA or anti-BSA with UV detection. When fluorescein isothiocyanate-labeled BSA (FITC-BSA) was used as tracer, the free and bound FITC-BSA were well separated giving definite peaks with laser induced fluorescence detection. The factors affecting the separation of the free and bound FITC-BSA, including voltage, pH and ionic strength of the running buffer, were systematically analyzed. Competitive CEIAs were demonstrated in uncoated and coated capillaries with whole or Fab fragment of the antibody. The coefficient of variation for the quantification of BSA in coated capillary was less than that in uncoated capillary. This study demonstrated that competitive CEIA could be applied to quantify high-molecular-mass protein in biological fluids. Copyright (C) 1999 Elsevier Science B.V.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Chromatography B: Biomedical Sciences and Applicationsen_US
dc.rightsJournal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences. Copyright © Elsevier BV.-
dc.subject.meshAntibodies, Monoclonal - Analysis - Immunologyen_US
dc.subject.meshAntigen-Antibody Complexen_US
dc.subject.meshElectrochemistryen_US
dc.subject.meshElectrophoresis, Capillary - Methodsen_US
dc.subject.meshFluorescein-5-Isothiocyanateen_US
dc.subject.meshImmunoassay - Methodsen_US
dc.subject.meshOsmolar Concentrationen_US
dc.subject.meshSerum Albumin, Bovine - Analysis - Immunologyen_US
dc.subject.meshSpectrometry, Fluorescenceen_US
dc.subject.meshSpectrophotometry, Ultravioleten_US
dc.titleUse of capillary electrophoresis-based competitive immunoassay for a large moleculeen_US
dc.typeArticleen_US
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0378-4347(99)00082-1en_US
dc.identifier.pmid10360423-
dc.identifier.scopuseid_2-s2.0-0032898329en_US
dc.identifier.hkuros42299-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032898329&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume727en_US
dc.identifier.issue1-2en_US
dc.identifier.spage63en_US
dc.identifier.epage71en_US
dc.identifier.isiWOS:000080170000008-
dc.identifier.scopusauthoridOu, JP=7202845297en_US
dc.identifier.scopusauthoridWang, QG=7406915351en_US
dc.identifier.scopusauthoridCheung, TM=7103334551en_US
dc.identifier.scopusauthoridChan, STH=24368283200en_US
dc.identifier.scopusauthoridYeung, WSB=7102370745en_US

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