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Article: Detection of T-cell receptor delta gene rearrangement by clonal specific polymerase chain reaction

TitleDetection of T-cell receptor delta gene rearrangement by clonal specific polymerase chain reaction
Authors
Issue Date1997
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leu
Citation
Leukemia, 1997, v. 11 SUPPL. 3, p. 281-284 How to Cite?
AbstractA sensitive and specific technique to detect minimal residual disease for T-cell malignancies was explored. Southern analysis and polymerase chain reaction (PCR) were used to detect the rearranged V-D-J segment of T-cell receptor delta (TCRδ) gene from malignant cell specimens of patients with leukemia and lymphoma of T-cell lineage. The PCR product was sequenced and from the DNA sequences of the V-D-J region, a 3′ antisense primer was designed and synthesized for clonal specific PCR (CS-PCR). Seven of the 22 T-ALL (32%) and 5 of 18 (28%) T-cell lymphoma showed clonal rearrangement by Southern analysis. Six of the 7 (86%) TALL and 4 of the 5 (80%) T-cell lymphoma which were Southern positive were also positive by TCRδ PCR. The PCR products of four cases of T-ALL showing clonal pattern by TCR5 PCR amplification were successfully sequenced and CS-PCR amplification performed. CS-PCR detected with confidence specific clonal rearrangement in a mixture containing 0.003% of malignant cells. Marrow specimens obtained at diagnosis and subsequent follow-ups from the 4 T-ALL patients were studied by Southern analysis, TCR5 PCR and CS-PCR. The first patient was in continuous morphological complete remission for more than 3 years and had persistently negative Southern, TCRδ PCR and CS-PCR results on follow-up. Initial follow-up marrow samples from the second patient had persistently positive CS-PCR results while they were still morphologically and TCRδ PCR negative and the patient had a frank leukemic relapse at 18 months. The other two patients had persistent disease by conventional morphological examination, Southern analysis, TCRδ PCR and CS-PCR studies were all positive as expected. CS-PCR is a highly specific and sensitive technique in detecting minimal residual disease for T-cell malignancies. Its potential applications warrant further clinical evaluation and correlation.
Persistent Identifierhttp://hdl.handle.net/10722/173226
ISSN
2015 Impact Factor: 12.104
2015 SCImago Journal Rankings: 5.142
References

 

DC FieldValueLanguage
dc.contributor.authorChan, DWen_US
dc.date.accessioned2012-10-30T06:28:37Z-
dc.date.available2012-10-30T06:28:37Z-
dc.date.issued1997en_US
dc.identifier.citationLeukemia, 1997, v. 11 SUPPL. 3, p. 281-284en_US
dc.identifier.issn0887-6924en_US
dc.identifier.urihttp://hdl.handle.net/10722/173226-
dc.description.abstractA sensitive and specific technique to detect minimal residual disease for T-cell malignancies was explored. Southern analysis and polymerase chain reaction (PCR) were used to detect the rearranged V-D-J segment of T-cell receptor delta (TCRδ) gene from malignant cell specimens of patients with leukemia and lymphoma of T-cell lineage. The PCR product was sequenced and from the DNA sequences of the V-D-J region, a 3′ antisense primer was designed and synthesized for clonal specific PCR (CS-PCR). Seven of the 22 T-ALL (32%) and 5 of 18 (28%) T-cell lymphoma showed clonal rearrangement by Southern analysis. Six of the 7 (86%) TALL and 4 of the 5 (80%) T-cell lymphoma which were Southern positive were also positive by TCRδ PCR. The PCR products of four cases of T-ALL showing clonal pattern by TCR5 PCR amplification were successfully sequenced and CS-PCR amplification performed. CS-PCR detected with confidence specific clonal rearrangement in a mixture containing 0.003% of malignant cells. Marrow specimens obtained at diagnosis and subsequent follow-ups from the 4 T-ALL patients were studied by Southern analysis, TCR5 PCR and CS-PCR. The first patient was in continuous morphological complete remission for more than 3 years and had persistently negative Southern, TCRδ PCR and CS-PCR results on follow-up. Initial follow-up marrow samples from the second patient had persistently positive CS-PCR results while they were still morphologically and TCRδ PCR negative and the patient had a frank leukemic relapse at 18 months. The other two patients had persistent disease by conventional morphological examination, Southern analysis, TCRδ PCR and CS-PCR studies were all positive as expected. CS-PCR is a highly specific and sensitive technique in detecting minimal residual disease for T-cell malignancies. Its potential applications warrant further clinical evaluation and correlation.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leuen_US
dc.relation.ispartofLeukemiaen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBone Marrow - Pathologyen_US
dc.subject.meshCloning, Molecular - Methodsen_US
dc.subject.meshDna Primersen_US
dc.subject.meshFollow-Up Studiesen_US
dc.subject.meshGene Rearrangement, Delta-Chain T-Cell Antigen Receptoren_US
dc.subject.meshHumansen_US
dc.subject.meshLeukemia, T-Cell - Genetics - Immunology - Mortality - Pathologyen_US
dc.subject.meshLeukemia-Lymphoma, Adult T-Cell - Genetics - Immunologyen_US
dc.subject.meshLymphoma, T-Cell - Genetics - Immunology - Mortality - Pathologyen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPolymerase Chain Reaction - Methodsen_US
dc.subject.meshPrognosisen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSurvival Rateen_US
dc.titleDetection of T-cell receptor delta gene rearrangement by clonal specific polymerase chain reactionen_US
dc.typeArticleen_US
dc.identifier.emailChan, DW:dwchan@hkucc.hku.hken_US
dc.identifier.authorityChan, DW=rp00543en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9209365-
dc.identifier.scopuseid_2-s2.0-0030816197en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030816197&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume11en_US
dc.identifier.issueSUPPL. 3en_US
dc.identifier.spage281en_US
dc.identifier.epage284en_US
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridChan, DW=26533900600en_US

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