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Article: The production of interleukin-1α immunoreactivity by human oviductal cells in a coculture system

TitleThe production of interleukin-1α immunoreactivity by human oviductal cells in a coculture system
Authors
Issue Date1996
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1058-0468
Citation
Journal Of Assisted Reproduction And Genetics, 1996, v. 13 n. 10, p. 772-775 How to Cite?
AbstractPurpose: The aim of this study was to determine the concentration of interleukin-1α in human embryo culture medium with or without oviductal cell coculture and to correlate the interleukin-1α levels with pregnancy. Methods: Culture media from 32 in vitro fertilization and embryo transfer cycles were assayed for interleukin-1α by immunoassay technique. Human embryos were cultured in Earles' balanced salt solution supplemented with 15% preovulatory serum (sEBSS) in 16 of these cycles, while embryos in the rest of the cycles were cocultured with human oviductal cells in sEBSS. Results: Both sEBSS and spent sEBSS after embryo culture contained low or undetectable levels of interleukin-1α in the pregnant and nonpregnant cycles. On the other hand, oviductal cells significantly increased the amount of interleukin-1α immunoreactivity in the conventional culture medium or coculture medium (P < 0.001, Mann-Whitney rank sum test). The concentrations of interleukin-1α in the spent sEBSS after oviductal cell culture and after coculture with human embryos were 1.5 ± 1.0 and 1.3 ± 0.9 pg/ml, respectively. There was no difference in the interleukin-1α concentration between the pregnant and the nonpregnant coculture cycles. Conclusions: These data showed that human oviductal cells produced interleukin-1α immunoreactivity in a coculture system. However, this production could not be used as a marker for successful embryo implantation.
Persistent Identifierhttp://hdl.handle.net/10722/173225
ISSN
2015 Impact Factor: 1.858
2015 SCImago Journal Rankings: 0.858
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYeung, WSBen_US
dc.contributor.authorLau, EYLen_US
dc.contributor.authorChan, AYFen_US
dc.contributor.authorPakChung, HOen_US
dc.date.accessioned2012-10-30T06:28:37Z-
dc.date.available2012-10-30T06:28:37Z-
dc.date.issued1996en_US
dc.identifier.citationJournal Of Assisted Reproduction And Genetics, 1996, v. 13 n. 10, p. 772-775en_US
dc.identifier.issn1058-0468en_US
dc.identifier.urihttp://hdl.handle.net/10722/173225-
dc.description.abstractPurpose: The aim of this study was to determine the concentration of interleukin-1α in human embryo culture medium with or without oviductal cell coculture and to correlate the interleukin-1α levels with pregnancy. Methods: Culture media from 32 in vitro fertilization and embryo transfer cycles were assayed for interleukin-1α by immunoassay technique. Human embryos were cultured in Earles' balanced salt solution supplemented with 15% preovulatory serum (sEBSS) in 16 of these cycles, while embryos in the rest of the cycles were cocultured with human oviductal cells in sEBSS. Results: Both sEBSS and spent sEBSS after embryo culture contained low or undetectable levels of interleukin-1α in the pregnant and nonpregnant cycles. On the other hand, oviductal cells significantly increased the amount of interleukin-1α immunoreactivity in the conventional culture medium or coculture medium (P < 0.001, Mann-Whitney rank sum test). The concentrations of interleukin-1α in the spent sEBSS after oviductal cell culture and after coculture with human embryos were 1.5 ± 1.0 and 1.3 ± 0.9 pg/ml, respectively. There was no difference in the interleukin-1α concentration between the pregnant and the nonpregnant coculture cycles. Conclusions: These data showed that human oviductal cells produced interleukin-1α immunoreactivity in a coculture system. However, this production could not be used as a marker for successful embryo implantation.en_US
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1058-0468en_US
dc.relation.ispartofJournal of Assisted Reproduction and Geneticsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCoculture Techniquesen_US
dc.subject.meshCulture Media - Chemistry - Metabolismen_US
dc.subject.meshEmbryo Transferen_US
dc.subject.meshEmbryo, Mammalian - Metabolismen_US
dc.subject.meshFallopian Tubes - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshFertilization In Vitroen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoassayen_US
dc.subject.meshInterleukin-1 - Analysis - Metabolismen_US
dc.subject.meshPregnancyen_US
dc.subject.meshPregnancy Outcomeen_US
dc.titleThe production of interleukin-1α immunoreactivity by human oviductal cells in a coculture systemen_US
dc.typeArticleen_US
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_US
dc.identifier.emailPakChung, HO:pcho@hku.hken_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.identifier.authorityPakChung, HO=rp00325en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF02066496en_US
dc.identifier.pmid8986587-
dc.identifier.scopuseid_2-s2.0-0030458479en_US
dc.identifier.hkuros22748-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030458479&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume13en_US
dc.identifier.issue10en_US
dc.identifier.spage772en_US
dc.identifier.epage775en_US
dc.identifier.isiWOS:A1996VZ79500005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYeung, WSB=7102370745en_US
dc.identifier.scopusauthoridLau, EYL=7103086093en_US
dc.identifier.scopusauthoridChan, AYF=16945898100en_US
dc.identifier.scopusauthoridPakChung, HO=7402211440en_US

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