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Article: Improved development of human embryos in vitro by a human oviductal cell co-culture system

TitleImproved development of human embryos in vitro by a human oviductal cell co-culture system
Authors
Issue Date1992
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 1992, v. 7 n. 8, p. 1144-1149 How to Cite?
AbstractThis study was undertaken to determine the effect of co-culture with human oviductal cells on human embryos. Spare embryos from gamete intra-Fallopian transfer (GIFT), pronuclear stage transfer (PROST) and in-vitro fertilization/embryo transfer (IVF/ET) programmes were either cultured in serum-supplemented Earle's balanced salt solution alone, or co-cultured in the same solution with oviductal cells from the pronuclear stage (day 1 post-insemination) or two-to four-cell stage (day 2 post-insemination). The co-cultured embryos appeared to have a higher developmental potential (higher rate of blastocyst formation and lower fragmentation rate), although there was no statistical difference in their rate of development, degree of fragmentation and stages attained, when compared with conventionally cultured embryos. The percentage of hatching blastocysts was significantly higher (P < 0.05, Fisher's exact test) for embryos co-cultured from day 1 post-insemination (38%) than for embryos which had not been co-cultured (7%). The blastocyst hatching rate for embryos co-cultured from day 2 post-insemination was 15%. It was therefore concluded that co-culture of human embryos with oviductal cells could improve the development of the embryos in vitro. The degree of improvement was more pronounced when the co-culture started at an earlier stage.
Persistent Identifierhttp://hdl.handle.net/10722/173180
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYeung, WSBen_US
dc.contributor.authorHo, PCen_US
dc.contributor.authorLau, EYLen_US
dc.contributor.authorChan, STHen_US
dc.date.accessioned2012-10-30T06:28:22Z-
dc.date.available2012-10-30T06:28:22Z-
dc.date.issued1992en_US
dc.identifier.citationHuman Reproduction, 1992, v. 7 n. 8, p. 1144-1149en_US
dc.identifier.issn0268-1161en_US
dc.identifier.urihttp://hdl.handle.net/10722/173180-
dc.description.abstractThis study was undertaken to determine the effect of co-culture with human oviductal cells on human embryos. Spare embryos from gamete intra-Fallopian transfer (GIFT), pronuclear stage transfer (PROST) and in-vitro fertilization/embryo transfer (IVF/ET) programmes were either cultured in serum-supplemented Earle's balanced salt solution alone, or co-cultured in the same solution with oviductal cells from the pronuclear stage (day 1 post-insemination) or two-to four-cell stage (day 2 post-insemination). The co-cultured embryos appeared to have a higher developmental potential (higher rate of blastocyst formation and lower fragmentation rate), although there was no statistical difference in their rate of development, degree of fragmentation and stages attained, when compared with conventionally cultured embryos. The percentage of hatching blastocysts was significantly higher (P < 0.05, Fisher's exact test) for embryos co-cultured from day 1 post-insemination (38%) than for embryos which had not been co-cultured (7%). The blastocyst hatching rate for embryos co-cultured from day 2 post-insemination was 15%. It was therefore concluded that co-culture of human embryos with oviductal cells could improve the development of the embryos in vitro. The degree of improvement was more pronounced when the co-culture started at an earlier stage.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_US
dc.relation.ispartofHuman Reproductionen_US
dc.subject.meshBlastocyst - Physiologyen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshCulture Techniquesen_US
dc.subject.meshFallopian Tubes - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshZygote - Physiologyen_US
dc.titleImproved development of human embryos in vitro by a human oviductal cell co-culture systemen_US
dc.typeArticleen_US
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_US
dc.identifier.emailHo, PC:pcho@hku.hken_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.identifier.authorityHo, PC=rp00325en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1400940-
dc.identifier.scopuseid_2-s2.0-0026702467en_US
dc.identifier.volume7en_US
dc.identifier.issue8en_US
dc.identifier.spage1144en_US
dc.identifier.epage1149en_US
dc.identifier.isiWOS:A1992JQ59900022-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridYeung, WSB=7102370745en_US
dc.identifier.scopusauthoridHo, PC=7402211440en_US
dc.identifier.scopusauthoridLau, EYL=7103086093en_US
dc.identifier.scopusauthoridChan, STH=24368283200en_US

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