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Article: Different evaluation of human sperm hypoosmotic swelling test and its relationship with the outcome of in-vitro fertilization of human occytes

TitleDifferent evaluation of human sperm hypoosmotic swelling test and its relationship with the outcome of in-vitro fertilization of human occytes
Authors
Issue Date1990
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 1990, v. 5 n. 1, p. 84-88 How to Cite?
AbstractThe hypoosmotic swelling test is a simple laboratory test to measure the functional integrity of the human sperm membrane, Its in-vivo and in-vitro applicability needs to be evaluated before it can be accepted as a useful routine test for the fertilizing potential of human seemen. We studied the standard semen analysis results and differential sperm tail swelling patterns of seminal and swim-up sperm after hypoosmotic treatment in 97 semen samples used for in-vitro fertilization of human oocytes. Semen samples were classified as infertile (0% fertilization rate; n = 27) or fertile ( > 0% fertilization rate; n = 70) before statistical analyses. There was a significant difference (P < 0.005) in percentage normal morphology of seminal sperm between the fertile and infertile semen samples. The percentage normal morpholgy of seminal sperm correlated (r = 0.4250; P < 0.005) with the in-vitro fertilization rate of human oocytes and this parameter was selected by the multivariate stepwise discriminant analysis as the discriminator capable of predicting the in-vitro fertilization rate with 57.7% accuracy. The percentage total swelling of seminal and swim-up sperm after hypoosmotic treatment was not correlated with the in-vitro fertilization rate. The percentage swelling pattern g (the open type) of seminal sperm was also selected by the multivaiate stepwise discriminant analysis as the discriminator to predict the in-vitro fertilization rate. This parameter correlated with the percentage normal morphology of seminal sperm (r = 0.3014; P < 0.05). The addition of this parameter to the percentage normal morphology of seminal sperm, another discriminator, did not significantly improve the predicitve accuracy (61.9%) for the in-vitro fertilization rate. Our results indicate that the addition of differential evaluation of the human sperm hypoosmotic swelling test to standard semen analysis is not helpful in predicting the in-vitro fertilization of human oocytes.
Persistent Identifierhttp://hdl.handle.net/10722/173163
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271

 

DC FieldValueLanguage
dc.contributor.authorChan, SYWen_US
dc.contributor.authorWang, Cen_US
dc.contributor.authorChan, STHen_US
dc.contributor.authorHo, PCen_US
dc.date.accessioned2012-10-30T06:28:16Z-
dc.date.available2012-10-30T06:28:16Z-
dc.date.issued1990en_US
dc.identifier.citationHuman Reproduction, 1990, v. 5 n. 1, p. 84-88en_US
dc.identifier.issn0268-1161en_US
dc.identifier.urihttp://hdl.handle.net/10722/173163-
dc.description.abstractThe hypoosmotic swelling test is a simple laboratory test to measure the functional integrity of the human sperm membrane, Its in-vivo and in-vitro applicability needs to be evaluated before it can be accepted as a useful routine test for the fertilizing potential of human seemen. We studied the standard semen analysis results and differential sperm tail swelling patterns of seminal and swim-up sperm after hypoosmotic treatment in 97 semen samples used for in-vitro fertilization of human oocytes. Semen samples were classified as infertile (0% fertilization rate; n = 27) or fertile ( > 0% fertilization rate; n = 70) before statistical analyses. There was a significant difference (P < 0.005) in percentage normal morphology of seminal sperm between the fertile and infertile semen samples. The percentage normal morpholgy of seminal sperm correlated (r = 0.4250; P < 0.005) with the in-vitro fertilization rate of human oocytes and this parameter was selected by the multivariate stepwise discriminant analysis as the discriminator capable of predicting the in-vitro fertilization rate with 57.7% accuracy. The percentage total swelling of seminal and swim-up sperm after hypoosmotic treatment was not correlated with the in-vitro fertilization rate. The percentage swelling pattern g (the open type) of seminal sperm was also selected by the multivaiate stepwise discriminant analysis as the discriminator to predict the in-vitro fertilization rate. This parameter correlated with the percentage normal morphology of seminal sperm (r = 0.3014; P < 0.05). The addition of this parameter to the percentage normal morphology of seminal sperm, another discriminator, did not significantly improve the predicitve accuracy (61.9%) for the in-vitro fertilization rate. Our results indicate that the addition of differential evaluation of the human sperm hypoosmotic swelling test to standard semen analysis is not helpful in predicting the in-vitro fertilization of human oocytes.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_US
dc.relation.ispartofHuman Reproductionen_US
dc.titleDifferent evaluation of human sperm hypoosmotic swelling test and its relationship with the outcome of in-vitro fertilization of human occytesen_US
dc.typeArticleen_US
dc.identifier.emailHo, PC:pcho@hku.hken_US
dc.identifier.authorityHo, PC=rp00325en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0025193365en_US
dc.identifier.volume5en_US
dc.identifier.issue1en_US
dc.identifier.spage84en_US
dc.identifier.epage88en_US
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridChan, SYW=7404255960en_US
dc.identifier.scopusauthoridWang, C=7501631357en_US
dc.identifier.scopusauthoridChan, STH=24368283200en_US
dc.identifier.scopusauthoridHo, PC=7402211440en_US

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