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Article: Oxytocin in Leydig cells: An immunocytochemical study of Percoll-purified cells from rat testes

TitleOxytocin in Leydig cells: An immunocytochemical study of Percoll-purified cells from rat testes
Authors
Issue Date1988
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
Citation
Cell And Tissue Research, 1988, v. 253 n. 2, p. 463-468 How to Cite?
AbstractTesticular interstitial cells, from rats aged 35 days, were dispersed with collagenase and separated through Percoll into 5 fractions (I-V); fraction I being the least dense. Measurement of basal testosterone production, histo-enzymological staining for 3β-hydroxysteroid dehydrogenase activity and electron microscopy indicated that the majority of Leydig cells were found in fraction IV (corresponding to a density of 1.076-1.097 g/ml). In addition, cells from this fraction responded to hCG treatment in a dose-dependent manner on day 0 and remained responsive after being cultured for 1 day. Immunostaining for oxytocin indicated that this fraction also contained the majority of the oxytocin-immunoreactive cells. On day 1 of culture, 56% of the cell population from fraction IV were positively stained for the steroidogenic enzyme and 75% immunoreactive for oxytocin. This overlap indicates that the Leydig cells were also the oxytocin immunoreactive cells.
Persistent Identifierhttp://hdl.handle.net/10722/173129
ISSN
2015 Impact Factor: 2.948
2015 SCImago Journal Rankings: 1.539
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYeung, WSBen_US
dc.contributor.authorGuldenaar, SEFen_US
dc.contributor.authorWorley, RTSen_US
dc.contributor.authorHumphrys, Jen_US
dc.contributor.authorPickering, BTen_US
dc.date.accessioned2012-10-30T06:28:05Z-
dc.date.available2012-10-30T06:28:05Z-
dc.date.issued1988en_US
dc.identifier.citationCell And Tissue Research, 1988, v. 253 n. 2, p. 463-468en_US
dc.identifier.issn0302-766Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/173129-
dc.description.abstractTesticular interstitial cells, from rats aged 35 days, were dispersed with collagenase and separated through Percoll into 5 fractions (I-V); fraction I being the least dense. Measurement of basal testosterone production, histo-enzymological staining for 3β-hydroxysteroid dehydrogenase activity and electron microscopy indicated that the majority of Leydig cells were found in fraction IV (corresponding to a density of 1.076-1.097 g/ml). In addition, cells from this fraction responded to hCG treatment in a dose-dependent manner on day 0 and remained responsive after being cultured for 1 day. Immunostaining for oxytocin indicated that this fraction also contained the majority of the oxytocin-immunoreactive cells. On day 1 of culture, 56% of the cell population from fraction IV were positively stained for the steroidogenic enzyme and 75% immunoreactive for oxytocin. This overlap indicates that the Leydig cells were also the oxytocin immunoreactive cells.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htmen_US
dc.relation.ispartofCell and Tissue Researchen_US
dc.subject.mesh3-Hydroxysteroid Dehydrogenases - Analysisen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Separationen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshLeydig Cells - Analysis - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshOxytocin - Analysisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshSubcellular Fractions - Analysisen_US
dc.subject.meshTestosterone - Metabolismen_US
dc.titleOxytocin in Leydig cells: An immunocytochemical study of Percoll-purified cells from rat testesen_US
dc.typeArticleen_US
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3165720-
dc.identifier.scopuseid_2-s2.0-0023878183en_US
dc.identifier.volume253en_US
dc.identifier.issue2en_US
dc.identifier.spage463en_US
dc.identifier.epage468en_US
dc.identifier.isiWOS:A1988P084800024-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridYeung, WSB=7102370745en_US
dc.identifier.scopusauthoridGuldenaar, SEF=6603836629en_US
dc.identifier.scopusauthoridWorley, RTS=7004610195en_US
dc.identifier.scopusauthoridHumphrys, J=6506260217en_US
dc.identifier.scopusauthoridPickering, BT=7005767182en_US

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