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- Scopus: eid_2-s2.0-0023878183
- PMID: 3165720
- WOS: WOS:A1988P084800024
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Article: Oxytocin in Leydig cells: An immunocytochemical study of Percoll-purified cells from rat testes
Title | Oxytocin in Leydig cells: An immunocytochemical study of Percoll-purified cells from rat testes |
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Authors | |
Keywords | 3 β-Hydroxysteroid dehydrogenase Leydig cells Oxytocin Rat (Wistar) Testis Testosterone |
Issue Date | 1988 |
Publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm |
Citation | Cell And Tissue Research, 1988, v. 253 n. 2, p. 463-468 How to Cite? |
Abstract | Testicular interstitial cells, from rats aged 35 days, were dispersed with collagenase and separated through Percoll into 5 fractions (I-V); fraction I being the least dense. Measurement of basal testosterone production, histo-enzymological staining for 3β-hydroxysteroid dehydrogenase activity and electron microscopy indicated that the majority of Leydig cells were found in fraction IV (corresponding to a density of 1.076-1.097 g/ml). In addition, cells from this fraction responded to hCG treatment in a dose-dependent manner on day 0 and remained responsive after being cultured for 1 day. Immunostaining for oxytocin indicated that this fraction also contained the majority of the oxytocin-immunoreactive cells. On day 1 of culture, 56% of the cell population from fraction IV were positively stained for the steroidogenic enzyme and 75% immunoreactive for oxytocin. This overlap indicates that the Leydig cells were also the oxytocin immunoreactive cells. |
Persistent Identifier | http://hdl.handle.net/10722/173129 |
ISSN | 2023 Impact Factor: 3.2 2023 SCImago Journal Rankings: 0.965 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Yeung, WSB | en_US |
dc.contributor.author | Guldenaar, SEF | en_US |
dc.contributor.author | Worley, RTS | en_US |
dc.contributor.author | Humphrys, J | en_US |
dc.contributor.author | Pickering, BT | en_US |
dc.date.accessioned | 2012-10-30T06:28:05Z | - |
dc.date.available | 2012-10-30T06:28:05Z | - |
dc.date.issued | 1988 | en_US |
dc.identifier.citation | Cell And Tissue Research, 1988, v. 253 n. 2, p. 463-468 | en_US |
dc.identifier.issn | 0302-766X | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/173129 | - |
dc.description.abstract | Testicular interstitial cells, from rats aged 35 days, were dispersed with collagenase and separated through Percoll into 5 fractions (I-V); fraction I being the least dense. Measurement of basal testosterone production, histo-enzymological staining for 3β-hydroxysteroid dehydrogenase activity and electron microscopy indicated that the majority of Leydig cells were found in fraction IV (corresponding to a density of 1.076-1.097 g/ml). In addition, cells from this fraction responded to hCG treatment in a dose-dependent manner on day 0 and remained responsive after being cultured for 1 day. Immunostaining for oxytocin indicated that this fraction also contained the majority of the oxytocin-immunoreactive cells. On day 1 of culture, 56% of the cell population from fraction IV were positively stained for the steroidogenic enzyme and 75% immunoreactive for oxytocin. This overlap indicates that the Leydig cells were also the oxytocin immunoreactive cells. | en_US |
dc.language | eng | en_US |
dc.publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm | en_US |
dc.relation.ispartof | Cell and Tissue Research | en_US |
dc.subject | 3 β-Hydroxysteroid dehydrogenase | - |
dc.subject | Leydig cells | - |
dc.subject | Oxytocin | - |
dc.subject | Rat (Wistar) | - |
dc.subject | Testis | - |
dc.subject | Testosterone | - |
dc.subject.mesh | 3-Hydroxysteroid Dehydrogenases - Analysis | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Cell Separation | en_US |
dc.subject.mesh | Immunohistochemistry | en_US |
dc.subject.mesh | Leydig Cells - Analysis - Metabolism | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Oxytocin - Analysis | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Inbred Strains | en_US |
dc.subject.mesh | Subcellular Fractions - Analysis | en_US |
dc.subject.mesh | Testosterone - Metabolism | en_US |
dc.title | Oxytocin in Leydig cells: An immunocytochemical study of Percoll-purified cells from rat testes | en_US |
dc.type | Article | en_US |
dc.identifier.email | Yeung, WSB:wsbyeung@hkucc.hku.hk | en_US |
dc.identifier.authority | Yeung, WSB=rp00331 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 3165720 | - |
dc.identifier.scopus | eid_2-s2.0-0023878183 | en_US |
dc.identifier.volume | 253 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 463 | en_US |
dc.identifier.epage | 468 | en_US |
dc.identifier.isi | WOS:A1988P084800024 | - |
dc.publisher.place | Germany | en_US |
dc.identifier.scopusauthorid | Yeung, WSB=7102370745 | en_US |
dc.identifier.scopusauthorid | Guldenaar, SEF=6603836629 | en_US |
dc.identifier.scopusauthorid | Worley, RTS=7004610195 | en_US |
dc.identifier.scopusauthorid | Humphrys, J=6506260217 | en_US |
dc.identifier.scopusauthorid | Pickering, BT=7005767182 | en_US |
dc.identifier.issnl | 0302-766X | - |