File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Smoking and drinking can induce p15 methylation in the upper aerodigestive tract of healthy individuals and patients with head and neck squamous cell carcinoma

TitleSmoking and drinking can induce p15 methylation in the upper aerodigestive tract of healthy individuals and patients with head and neck squamous cell carcinoma
Authors
Issue Date2004
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741
Citation
Cancer, 2004, v. 101 n. 1, p. 125-132 How to Cite?
AbstractBACKGROUND. The consumption of tobacco and alcohol has been implicated in the development of head and neck squamous cell carcinoma (HNSCC). Promoter methylation of tumor suppressor genes is common in HNSCC. In this study, the authors evaluated the effects of tobacco and alcohol on p15 gene methylation of cells in cells from the mouth and throat of physically healthy individuals and patients with HNSCC. METHODS. The study participants were divided into 3 groups, including a group of 37 healthy nonsmokers and nondrinkers, a group of 22 healthy smokers and/or drinkers and a group of 31 patients with HNSCC. RESULTS. Methylation of p15 was detected in cells obtained from mouth and throat (M&T) rinsing fluid samples from 3 of 37 healthy individuals (8%) who did not drink or smoke, from 15 of 22 healthy smokers and/or drinkers (68%), and from 15 of 31 patients (48%) with HNSCC. Among 31 patients with HNSCC, 20 patients (65%) had methylated p15 gene in their tumor biopsies. With the use of β-actin as a reference, the ratio of methylated p15 to β-actin was calculated as an index of the percentage of cells with p15 methylation. The percentage of exfoliated cells from M&T rinsing fluid samples that had p15 methylation ranged from 0% to 11% for patients with HNSCC and from 0% to 21% for healthy smokers/drinkers, respectively. The methylation index of tumor cells with p15 methylation ranged from 0% to 65%. CONCLUSIONS. The results suggest that p15 gene methylation can be induced by chronic smoking and drinking and may play a role in the very early stages of carcinogenesis in HNSCC. © 2004 American Cancer Society.
Persistent Identifierhttp://hdl.handle.net/10722/172896
ISSN
2015 Impact Factor: 5.649
2015 SCImago Journal Rankings: 3.188
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChang, HWen_US
dc.contributor.authorLing, GSen_US
dc.contributor.authorWei, WIen_US
dc.contributor.authorYuen, APWen_US
dc.date.accessioned2012-10-30T06:25:38Z-
dc.date.available2012-10-30T06:25:38Z-
dc.date.issued2004en_US
dc.identifier.citationCancer, 2004, v. 101 n. 1, p. 125-132en_US
dc.identifier.issn0008-543Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/172896-
dc.description.abstractBACKGROUND. The consumption of tobacco and alcohol has been implicated in the development of head and neck squamous cell carcinoma (HNSCC). Promoter methylation of tumor suppressor genes is common in HNSCC. In this study, the authors evaluated the effects of tobacco and alcohol on p15 gene methylation of cells in cells from the mouth and throat of physically healthy individuals and patients with HNSCC. METHODS. The study participants were divided into 3 groups, including a group of 37 healthy nonsmokers and nondrinkers, a group of 22 healthy smokers and/or drinkers and a group of 31 patients with HNSCC. RESULTS. Methylation of p15 was detected in cells obtained from mouth and throat (M&T) rinsing fluid samples from 3 of 37 healthy individuals (8%) who did not drink or smoke, from 15 of 22 healthy smokers and/or drinkers (68%), and from 15 of 31 patients (48%) with HNSCC. Among 31 patients with HNSCC, 20 patients (65%) had methylated p15 gene in their tumor biopsies. With the use of β-actin as a reference, the ratio of methylated p15 to β-actin was calculated as an index of the percentage of cells with p15 methylation. The percentage of exfoliated cells from M&T rinsing fluid samples that had p15 methylation ranged from 0% to 11% for patients with HNSCC and from 0% to 21% for healthy smokers/drinkers, respectively. The methylation index of tumor cells with p15 methylation ranged from 0% to 65%. CONCLUSIONS. The results suggest that p15 gene methylation can be induced by chronic smoking and drinking and may play a role in the very early stages of carcinogenesis in HNSCC. © 2004 American Cancer Society.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741en_US
dc.relation.ispartofCanceren_US
dc.rightsCancer. Copyright © John Wiley & Sons, Inc.-
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshAlcohol Drinking - Adverse Effectsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCarcinoma, Squamous Cell - Etiology - Genetics - Pathologyen_US
dc.subject.meshDna Methylationen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenes, Tumor Suppressor - Drug Effectsen_US
dc.subject.meshHead And Neck Neoplasms - Etiology - Genetics - Pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMouth - Drug Effects - Pathologyen_US
dc.subject.meshPharynx - Drug Effects - Pathologyen_US
dc.subject.meshPromoter Regions, Genetic - Drug Effectsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSmoking - Adverse Effectsen_US
dc.titleSmoking and drinking can induce p15 methylation in the upper aerodigestive tract of healthy individuals and patients with head and neck squamous cell carcinomaen_US
dc.typeArticleen_US
dc.identifier.emailWei, WI: hrmswwi@hku.hken_US
dc.identifier.authorityWei, WI=rp00323en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1002/cncr.20323en_US
dc.identifier.pmid15221997-
dc.identifier.scopuseid_2-s2.0-3042580177en_US
dc.identifier.hkuros94575-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-3042580177&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume101en_US
dc.identifier.issue1en_US
dc.identifier.spage125en_US
dc.identifier.epage132en_US
dc.identifier.isiWOS:000222134200014-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChang, HW=7407523249en_US
dc.identifier.scopusauthoridLing, GS=24577720400en_US
dc.identifier.scopusauthoridWei, WI=7403321552en_US
dc.identifier.scopusauthoridYuen, APW=7006290111en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats