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Article: Novel use of the Friedewald formula to tackle anomalous HDL-C results in two cases of paraproteinaemia
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TitleNovel use of the Friedewald formula to tackle anomalous HDL-C results in two cases of paraproteinaemia
 
AuthorsPang, RWC1
Tam, SCF1 1
 
Issue Date2004
 
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/clinbiochem
 
CitationClinical Biochemistry, 2004, v. 37 n. 3, p. 238-240 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.clinbiochem.2003.11.003
 
AbstractObjectives: We report here two cases of paraproteinaemia with one falsely low and the other dubiously high HDL-cholesterol (HDL-C) results. The spurious results seemed to be related to the nature (IgG or IgM) as well as tshe concentration of the paraproteins. Design and methods: We have been using an alternative approach to estimate the HDL-C concentration by incorporating into it the LDL-cholesterol (LDL-C) value obtained by direct measurements and by back-calculation based on the time-honored Friedewald equation in these atypical specimens as an interim measure, pending optimization of the Roche direct HDL-C plus assay currently in use in our laboratory. Results: This approach is convenient and does not require sophisticated instrumentation. What we are suggesting is to tackle this analytical problems on HDL-C assay due to paraprotein interference by back-calculating the HDL-C values from the measured LDL-C and triglyceride values using the Friedewald formula and is to be regarded as an alternative way to circumvent the interference issue without the need for more elaborative laboratory procedures. We do not intend to advocate screening every single HDL-C value obtained by the direct method for possible analytical errors using this approach. Conclusions: The back-calculation for HDL-C based on the Friedewald formula is conceived by the authors as an alternative and relatively simple way to estimate the HDL-C value in the presence of paraprotein interference, in particular when there is a minus HDL-C value or when the result is dubiously high. By the same token, when the measured HDL-C and the calculated HDL-C do not match further investigations would be warranted to safeguard the validity of the reported result. It is also, to the best of our knowledge, the first time extra bands due to the IgM and IgG paraproteins were demonstrated in the lipoprotein electrophoresis plate. © 2003 The Canadian Society of Clinical Chemists. All rights reserved.
 
ISSN0009-9120
2012 Impact Factor: 2.45
2012 SCImago Journal Rankings: 0.654
 
DOIhttp://dx.doi.org/10.1016/j.clinbiochem.2003.11.003
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorPang, RWC
 
dc.contributor.authorTam, SCF
 
dc.date.accessioned2012-10-30T06:25:21Z
 
dc.date.available2012-10-30T06:25:21Z
 
dc.date.issued2004
 
dc.description.abstractObjectives: We report here two cases of paraproteinaemia with one falsely low and the other dubiously high HDL-cholesterol (HDL-C) results. The spurious results seemed to be related to the nature (IgG or IgM) as well as tshe concentration of the paraproteins. Design and methods: We have been using an alternative approach to estimate the HDL-C concentration by incorporating into it the LDL-cholesterol (LDL-C) value obtained by direct measurements and by back-calculation based on the time-honored Friedewald equation in these atypical specimens as an interim measure, pending optimization of the Roche direct HDL-C plus assay currently in use in our laboratory. Results: This approach is convenient and does not require sophisticated instrumentation. What we are suggesting is to tackle this analytical problems on HDL-C assay due to paraprotein interference by back-calculating the HDL-C values from the measured LDL-C and triglyceride values using the Friedewald formula and is to be regarded as an alternative way to circumvent the interference issue without the need for more elaborative laboratory procedures. We do not intend to advocate screening every single HDL-C value obtained by the direct method for possible analytical errors using this approach. Conclusions: The back-calculation for HDL-C based on the Friedewald formula is conceived by the authors as an alternative and relatively simple way to estimate the HDL-C value in the presence of paraprotein interference, in particular when there is a minus HDL-C value or when the result is dubiously high. By the same token, when the measured HDL-C and the calculated HDL-C do not match further investigations would be warranted to safeguard the validity of the reported result. It is also, to the best of our knowledge, the first time extra bands due to the IgM and IgG paraproteins were demonstrated in the lipoprotein electrophoresis plate. © 2003 The Canadian Society of Clinical Chemists. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationClinical Biochemistry, 2004, v. 37 n. 3, p. 238-240 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.clinbiochem.2003.11.003
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.clinbiochem.2003.11.003
 
dc.identifier.epage240
 
dc.identifier.issn0009-9120
2012 Impact Factor: 2.45
2012 SCImago Journal Rankings: 0.654
 
dc.identifier.issue3
 
dc.identifier.pmid14972647
 
dc.identifier.scopuseid_2-s2.0-1242314863
 
dc.identifier.spage238
 
dc.identifier.urihttp://hdl.handle.net/10722/172855
 
dc.identifier.volume37
 
dc.languageeng
 
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/clinbiochem
 
dc.publisher.placeUnited States
 
dc.relation.ispartofClinical Biochemistry
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAged
 
dc.subject.meshCholesterol, Hdl - Blood
 
dc.subject.meshElectrophoresis - Methods
 
dc.subject.meshFemale
 
dc.subject.meshHumans
 
dc.subject.meshMale
 
dc.subject.meshMiddle Aged
 
dc.subject.meshParaproteinemias - Blood
 
dc.titleNovel use of the Friedewald formula to tackle anomalous HDL-C results in two cases of paraproteinaemia
 
dc.typeArticle
 
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Author Affiliations
  1. Queen Mary Hospital Hong Kong