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Article: Novel use of the Friedewald formula to tackle anomalous HDL-C results in two cases of paraproteinaemia

TitleNovel use of the Friedewald formula to tackle anomalous HDL-C results in two cases of paraproteinaemia
Authors
Issue Date2004
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/clinbiochem
Citation
Clinical Biochemistry, 2004, v. 37 n. 3, p. 238-240 How to Cite?
AbstractObjectives: We report here two cases of paraproteinaemia with one falsely low and the other dubiously high HDL-cholesterol (HDL-C) results. The spurious results seemed to be related to the nature (IgG or IgM) as well as tshe concentration of the paraproteins. Design and methods: We have been using an alternative approach to estimate the HDL-C concentration by incorporating into it the LDL-cholesterol (LDL-C) value obtained by direct measurements and by back-calculation based on the time-honored Friedewald equation in these atypical specimens as an interim measure, pending optimization of the Roche direct HDL-C plus assay currently in use in our laboratory. Results: This approach is convenient and does not require sophisticated instrumentation. What we are suggesting is to tackle this analytical problems on HDL-C assay due to paraprotein interference by back-calculating the HDL-C values from the measured LDL-C and triglyceride values using the Friedewald formula and is to be regarded as an alternative way to circumvent the interference issue without the need for more elaborative laboratory procedures. We do not intend to advocate screening every single HDL-C value obtained by the direct method for possible analytical errors using this approach. Conclusions: The back-calculation for HDL-C based on the Friedewald formula is conceived by the authors as an alternative and relatively simple way to estimate the HDL-C value in the presence of paraprotein interference, in particular when there is a minus HDL-C value or when the result is dubiously high. By the same token, when the measured HDL-C and the calculated HDL-C do not match further investigations would be warranted to safeguard the validity of the reported result. It is also, to the best of our knowledge, the first time extra bands due to the IgM and IgG paraproteins were demonstrated in the lipoprotein electrophoresis plate. © 2003 The Canadian Society of Clinical Chemists. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/172855
ISSN
2014 Impact Factor: 2.275
2014 SCImago Journal Rankings: 0.725
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPang, RWCen_US
dc.contributor.authorTam, SCFen_US
dc.date.accessioned2012-10-30T06:25:21Z-
dc.date.available2012-10-30T06:25:21Z-
dc.date.issued2004en_US
dc.identifier.citationClinical Biochemistry, 2004, v. 37 n. 3, p. 238-240en_US
dc.identifier.issn0009-9120en_US
dc.identifier.urihttp://hdl.handle.net/10722/172855-
dc.description.abstractObjectives: We report here two cases of paraproteinaemia with one falsely low and the other dubiously high HDL-cholesterol (HDL-C) results. The spurious results seemed to be related to the nature (IgG or IgM) as well as tshe concentration of the paraproteins. Design and methods: We have been using an alternative approach to estimate the HDL-C concentration by incorporating into it the LDL-cholesterol (LDL-C) value obtained by direct measurements and by back-calculation based on the time-honored Friedewald equation in these atypical specimens as an interim measure, pending optimization of the Roche direct HDL-C plus assay currently in use in our laboratory. Results: This approach is convenient and does not require sophisticated instrumentation. What we are suggesting is to tackle this analytical problems on HDL-C assay due to paraprotein interference by back-calculating the HDL-C values from the measured LDL-C and triglyceride values using the Friedewald formula and is to be regarded as an alternative way to circumvent the interference issue without the need for more elaborative laboratory procedures. We do not intend to advocate screening every single HDL-C value obtained by the direct method for possible analytical errors using this approach. Conclusions: The back-calculation for HDL-C based on the Friedewald formula is conceived by the authors as an alternative and relatively simple way to estimate the HDL-C value in the presence of paraprotein interference, in particular when there is a minus HDL-C value or when the result is dubiously high. By the same token, when the measured HDL-C and the calculated HDL-C do not match further investigations would be warranted to safeguard the validity of the reported result. It is also, to the best of our knowledge, the first time extra bands due to the IgM and IgG paraproteins were demonstrated in the lipoprotein electrophoresis plate. © 2003 The Canadian Society of Clinical Chemists. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/clinbiochemen_US
dc.relation.ispartofClinical Biochemistryen_US
dc.subject.meshAgeden_US
dc.subject.meshCholesterol, Hdl - Blooden_US
dc.subject.meshElectrophoresis - Methodsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshParaproteinemias - Blooden_US
dc.titleNovel use of the Friedewald formula to tackle anomalous HDL-C results in two cases of paraproteinaemiaen_US
dc.typeArticleen_US
dc.identifier.emailPang, RWC: robertap@hkucc.hku.hken_US
dc.identifier.authorityPang, RWC=rp00274en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.clinbiochem.2003.11.003en_US
dc.identifier.pmid14972647-
dc.identifier.scopuseid_2-s2.0-1242314863en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1242314863&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume37en_US
dc.identifier.issue3en_US
dc.identifier.spage238en_US
dc.identifier.epage240en_US
dc.identifier.isiWOS:000220037900011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPang, RWC=7004376659en_US
dc.identifier.scopusauthoridTam, SCF=7202037323en_US

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