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Article: Embryotrophic factor-3 from human oviductal cells enhances proliferation, suppresses apoptosis and stimulates the expression of the β1 subunit of sodium-potassium ATPase in mouse embryos

TitleEmbryotrophic factor-3 from human oviductal cells enhances proliferation, suppresses apoptosis and stimulates the expression of the β1 subunit of sodium-potassium ATPase in mouse embryos
Authors
KeywordsApoptosis
Embryotrophic factor
Oviduct
Proliferation
Sodium-potassium ATPase
Issue Date2004
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 2004, v. 19 n. 12, p. 2919-2926 How to Cite?
AbstractBackground: Embrytrophic factor-3 (ETF-3) from human oviductal cells enhanced the development of mouse preimplantation embryos. This report studied the embryotrophic mechanisms of the molecule. Methods and results: Mouse embryos were incubated with ETF-3 for 24h at different stages of development. ETF-3 treatment between 96 and 120 h post-HCG increased the cell count of blastocysts, whilst treatment between 72 and 96 h post-HCG enhanced the expansion and hatching of the blastocysts. ETF-3 increased the cell number of the embryos by suppressing apoptosis and increasing proliferation as determined by TUNEL and bromodeoxyuridine uptake assays, respectively. Real-time quantitative PCR showed that the in vivo developed and ETF-3-treated blastocysts had a significantly higher mRNA copy number of Na/K-ATPase-β1, but not of hepsin, than that of blastocysts cultured in medium alone. The former gene was associated with cavitation of blastocysts while the latter was related to hatching of blastocyst. The beneficial effect of ETF-3 on blastocyst hatching was also seen when ETF-3-supplemented commercially available sequential culture medium for human embryo culture was used to culture mouse embryos. Conclusions: ETF-3 improves embryo development by enhancing proliferation, suppressing apoptosis and stimulating expression of genes related to blastocyst cavitation. Supplementating human embryo culture medium with ETF-3 may improve the success rate in clinical assisted reproduction. © European Society of Human Reproduction and Embryology 2004; all rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/172852
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, JSen_HK
dc.contributor.authorLee, YLen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorKwok, KLen_HK
dc.contributor.authorLee, WMen_HK
dc.contributor.authorLuk, JMen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2012-10-30T06:25:20Z-
dc.date.available2012-10-30T06:25:20Z-
dc.date.issued2004en_HK
dc.identifier.citationHuman Reproduction, 2004, v. 19 n. 12, p. 2919-2926en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/172852-
dc.description.abstractBackground: Embrytrophic factor-3 (ETF-3) from human oviductal cells enhanced the development of mouse preimplantation embryos. This report studied the embryotrophic mechanisms of the molecule. Methods and results: Mouse embryos were incubated with ETF-3 for 24h at different stages of development. ETF-3 treatment between 96 and 120 h post-HCG increased the cell count of blastocysts, whilst treatment between 72 and 96 h post-HCG enhanced the expansion and hatching of the blastocysts. ETF-3 increased the cell number of the embryos by suppressing apoptosis and increasing proliferation as determined by TUNEL and bromodeoxyuridine uptake assays, respectively. Real-time quantitative PCR showed that the in vivo developed and ETF-3-treated blastocysts had a significantly higher mRNA copy number of Na/K-ATPase-β1, but not of hepsin, than that of blastocysts cultured in medium alone. The former gene was associated with cavitation of blastocysts while the latter was related to hatching of blastocyst. The beneficial effect of ETF-3 on blastocyst hatching was also seen when ETF-3-supplemented commercially available sequential culture medium for human embryo culture was used to culture mouse embryos. Conclusions: ETF-3 improves embryo development by enhancing proliferation, suppressing apoptosis and stimulating expression of genes related to blastocyst cavitation. Supplementating human embryo culture medium with ETF-3 may improve the success rate in clinical assisted reproduction. © European Society of Human Reproduction and Embryology 2004; all rights reserved.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsHuman Reproduction. Copyright © Oxford University Press.-
dc.subjectApoptosisen_HK
dc.subjectEmbryotrophic factoren_HK
dc.subjectOviducten_HK
dc.subjectProliferationen_HK
dc.subjectSodium-potassium ATPaseen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosis - Drug Effectsen_US
dc.subject.meshBlastocyst - Cytology - Drug Effects - Physiologyen_US
dc.subject.meshCell Proliferation - Drug Effectsen_US
dc.subject.meshEmbryo Culture Techniquesen_US
dc.subject.meshEmbryonic Development - Drug Effects - Physiologyen_US
dc.subject.meshFallopian Tubes - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation, Developmentalen_US
dc.subject.meshGrowth Substances - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Strainsen_US
dc.subject.meshProtein Subunits - Drug Effects - Geneticsen_US
dc.subject.meshSerine Endopeptidases - Drug Effects - Geneticsen_US
dc.subject.meshSodium-Potassium-Exchanging Atpase - Drug Effects - Geneticsen_US
dc.titleEmbryotrophic factor-3 from human oviductal cells enhances proliferation, suppresses apoptosis and stimulates the expression of the β1 subunit of sodium-potassium ATPase in mouse embryosen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, YL: cherielee@hku.hken_HK
dc.identifier.emailLee, KF: ckflee@hku.hken_HK
dc.identifier.emailLee, WM: hrszlwm@hku.hken_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, YL=rp00308en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityLee, WM=rp00728en_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/humrep/deh497en_HK
dc.identifier.pmid15459171-
dc.identifier.scopuseid_2-s2.0-11144338757en_HK
dc.identifier.hkuros96766-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-11144338757&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue12en_HK
dc.identifier.spage2919en_HK
dc.identifier.epage2926en_HK
dc.identifier.isiWOS:000225254300034-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridXu, JS=7408556691en_HK
dc.identifier.scopusauthoridLee, YL=15033851800en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridKwok, KL=16645835100en_HK
dc.identifier.scopusauthoridLee, WM=24799156600en_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.citeulike44710-

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