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Article: The concentration of circulating corticotropin-releasing hormone mRNA in maternal plasma is increased in preeclampsia

TitleThe concentration of circulating corticotropin-releasing hormone mRNA in maternal plasma is increased in preeclampsia
Authors
Issue Date2003
PublisherAmerican Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.org
Citation
Clinical Chemistry, 2003, v. 49 n. 5, p. 727-731 How to Cite?
AbstractBackground: Increased fetal DNA in maternal plasma/serum has been reported in pregnancies complicated by preeclampsia. We hypothesize that fetal RNA may also be increased in maternal plasma in preeclampsia. Methods: We developed a real-time quantitative reverse transcription-PCR assay to measure the concentration of the mRNA of the corticotropin-releasing hormone (CRH) locus. Peripheral blood samples were obtained from healthy pregnant women both before and 2 h after delivery. Peripheral blood samples were also obtained from women suffering from preeclampsia and controls matched for gestational age. Plasma was harvested from these samples, and RNA was extracted. Plasma RNA was subjected to analysis by the reverse transcription-PCR assay. Results: CRH mRNA was detected in the plasma of 10 healthy pregnant women in the third trimester. CRH mRNA was found to be cleared very rapidly after cesarean section, with no detectable signal by 2 h postpartum. Plasma CRH mRNA concentrations were 1070 and 102 copies/mL, respectively, in 12 preeclamptic women and 10 healthy pregnant women matched for gestational age (Mann-Whitney test, P <0.001). Conclusion: Plasma CRH mRNA represents a new molecular marker for preeclampsia. Maternal plasma RNA is gender- and polymorphism-independent and may allow noninvasive gene-expression profiling of an unborn fetus. © 2003 American Association for Clinical Chemistry.
Persistent Identifierhttp://hdl.handle.net/10722/172841
ISSN
2015 Impact Factor: 7.457
2015 SCImago Journal Rankings: 2.472
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNg, EKOen_US
dc.contributor.authorLeung, TNen_US
dc.contributor.authorTsui, NBYen_US
dc.contributor.authorLau, TKen_US
dc.contributor.authorPanesar, NSen_US
dc.contributor.authorChiu, RWKen_US
dc.contributor.authorLo, YMDen_US
dc.date.accessioned2012-10-30T06:25:14Z-
dc.date.available2012-10-30T06:25:14Z-
dc.date.issued2003en_US
dc.identifier.citationClinical Chemistry, 2003, v. 49 n. 5, p. 727-731en_US
dc.identifier.issn0009-9147en_US
dc.identifier.urihttp://hdl.handle.net/10722/172841-
dc.description.abstractBackground: Increased fetal DNA in maternal plasma/serum has been reported in pregnancies complicated by preeclampsia. We hypothesize that fetal RNA may also be increased in maternal plasma in preeclampsia. Methods: We developed a real-time quantitative reverse transcription-PCR assay to measure the concentration of the mRNA of the corticotropin-releasing hormone (CRH) locus. Peripheral blood samples were obtained from healthy pregnant women both before and 2 h after delivery. Peripheral blood samples were also obtained from women suffering from preeclampsia and controls matched for gestational age. Plasma was harvested from these samples, and RNA was extracted. Plasma RNA was subjected to analysis by the reverse transcription-PCR assay. Results: CRH mRNA was detected in the plasma of 10 healthy pregnant women in the third trimester. CRH mRNA was found to be cleared very rapidly after cesarean section, with no detectable signal by 2 h postpartum. Plasma CRH mRNA concentrations were 1070 and 102 copies/mL, respectively, in 12 preeclamptic women and 10 healthy pregnant women matched for gestational age (Mann-Whitney test, P <0.001). Conclusion: Plasma CRH mRNA represents a new molecular marker for preeclampsia. Maternal plasma RNA is gender- and polymorphism-independent and may allow noninvasive gene-expression profiling of an unborn fetus. © 2003 American Association for Clinical Chemistry.en_US
dc.languageengen_US
dc.publisherAmerican Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.orgen_US
dc.relation.ispartofClinical Chemistryen_US
dc.subject.meshBlood Circulationen_US
dc.subject.meshCorticotropin-Releasing Hormone - Blood - Geneticsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMothersen_US
dc.subject.meshPostpartum Perioden_US
dc.subject.meshPre-Eclampsia - Blooden_US
dc.subject.meshPregnancyen_US
dc.subject.meshPregnancy Trimester, Thirden_US
dc.subject.meshRna, Messenger - Blooden_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.titleThe concentration of circulating corticotropin-releasing hormone mRNA in maternal plasma is increased in preeclampsiaen_US
dc.typeArticleen_US
dc.identifier.emailNg, EKO: ngko@hku.hken_US
dc.identifier.authorityNg, EKO=rp01364en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1373/49.5.727en_US
dc.identifier.pmid12709362-
dc.identifier.scopuseid_2-s2.0-0242432340en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0242432340&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume49en_US
dc.identifier.issue5en_US
dc.identifier.spage727en_US
dc.identifier.epage731en_US
dc.identifier.isiWOS:000182541900003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridNg, EKO=21135553700en_US
dc.identifier.scopusauthoridLeung, TN=7202110927en_US
dc.identifier.scopusauthoridTsui, NBY=6602401748en_US
dc.identifier.scopusauthoridLau, TK=24491963900en_US
dc.identifier.scopusauthoridPanesar, NS=7005824593en_US
dc.identifier.scopusauthoridChiu, RWK=7103038413en_US
dc.identifier.scopusauthoridLo, YMD=7401935391en_US

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