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Article: Embryotrophic factor-3 from human oviductal cells affects the messenger RNA expression of mouse blastocyst

TitleEmbryotrophic factor-3 from human oviductal cells affects the messenger RNA expression of mouse blastocyst
Authors
KeywordsEarly development
Embryo
Fallopian tubes
Gene regulation
Oviduct
Issue Date2003
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
Biology Of Reproduction, 2003, v. 68 n. 2, p. 375-382 How to Cite?
AbstractOur previous results showed that embryotrophic factor-3 (ETF-3) from human oviductal cells increased the size and hatching rate of mouse blastocysts in vitro. The present study investigated the production of ETF-3 by an immortalized human oviductal cell line (OE-E6/E7) and the effects of ETF-3 on the mRNA expression of mouse embryos. The ETF-3 was purified from primary oviductal cell conditioned media using sequential liquid chromatographic systems, and antiserum against ETF-3 was raised. The ETF-3-supplemented Chatot-Ziomek-Bavister medium was used to culture Day 1 MF1 × BALB/c mouse embryos for 4 days. The ETF-3 treatment significantly enhanced the mouse embryo blastulation and hatching rate. The antiserum, at concentrations of 0.03-3%, abolished the embryotrophic effect of ETF-3. Positive ETF-3 immunoreactivity was detected in the primary oviductal cells, OE-E6/E7, and blastocysts derived from ETF-3 treatment. Vero cells (African Green Monkey kidney cell line), fibroblasts, and embryos cultured in control medium did not possess ETF-3 immunoreactivity. The mRNA expression patterns of the treated embryos were studied at the blastocyst stage by mRNA differential display reverse transcription-polymerase chain reaction (DDRT-PCR). The DDRT-PCR showed that some of the mRNAs were differentially expressed after ETF-3 treatment. Twelve of the differentially expressed mRNAs that had high homology with cDNA sequences in the GenBank were selected for further characterization. The differential expression of seven of these mRNAs (ezrin, heat shock 70-kDa protein, cytochrome c oxidase subunit VIIa-L precursor, proteinase-activated receptor 2, eukaryotic translation initiation factor 2β, cullin 1, and proliferating cell nuclear antigen) was confirmed by semi-quantitative RT-PCR. In conclusion, immortalized oviductal cells produce ETF-3, which influences mRNA expression of mouse blastocyst.
Persistent Identifierhttp://hdl.handle.net/10722/172822
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, YLen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorXu, JSen_HK
dc.contributor.authorKwok, KLen_HK
dc.contributor.authorLuk, JMen_HK
dc.contributor.authorLee, WMen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2012-10-30T06:25:07Z-
dc.date.available2012-10-30T06:25:07Z-
dc.date.issued2003en_HK
dc.identifier.citationBiology Of Reproduction, 2003, v. 68 n. 2, p. 375-382en_HK
dc.identifier.issn0006-3363en_HK
dc.identifier.urihttp://hdl.handle.net/10722/172822-
dc.description.abstractOur previous results showed that embryotrophic factor-3 (ETF-3) from human oviductal cells increased the size and hatching rate of mouse blastocysts in vitro. The present study investigated the production of ETF-3 by an immortalized human oviductal cell line (OE-E6/E7) and the effects of ETF-3 on the mRNA expression of mouse embryos. The ETF-3 was purified from primary oviductal cell conditioned media using sequential liquid chromatographic systems, and antiserum against ETF-3 was raised. The ETF-3-supplemented Chatot-Ziomek-Bavister medium was used to culture Day 1 MF1 × BALB/c mouse embryos for 4 days. The ETF-3 treatment significantly enhanced the mouse embryo blastulation and hatching rate. The antiserum, at concentrations of 0.03-3%, abolished the embryotrophic effect of ETF-3. Positive ETF-3 immunoreactivity was detected in the primary oviductal cells, OE-E6/E7, and blastocysts derived from ETF-3 treatment. Vero cells (African Green Monkey kidney cell line), fibroblasts, and embryos cultured in control medium did not possess ETF-3 immunoreactivity. The mRNA expression patterns of the treated embryos were studied at the blastocyst stage by mRNA differential display reverse transcription-polymerase chain reaction (DDRT-PCR). The DDRT-PCR showed that some of the mRNAs were differentially expressed after ETF-3 treatment. Twelve of the differentially expressed mRNAs that had high homology with cDNA sequences in the GenBank were selected for further characterization. The differential expression of seven of these mRNAs (ezrin, heat shock 70-kDa protein, cytochrome c oxidase subunit VIIa-L precursor, proteinase-activated receptor 2, eukaryotic translation initiation factor 2β, cullin 1, and proliferating cell nuclear antigen) was confirmed by semi-quantitative RT-PCR. In conclusion, immortalized oviductal cells produce ETF-3, which influences mRNA expression of mouse blastocyst.en_HK
dc.languageengen_US
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_HK
dc.relation.ispartofBiology of Reproductionen_HK
dc.subjectEarly developmenten_HK
dc.subjectEmbryoen_HK
dc.subjectFallopian tubesen_HK
dc.subjectGene regulationen_HK
dc.subjectOviducten_HK
dc.subject.meshAnimalsen_US
dc.subject.meshBlastocyst - Drug Effects - Metabolismen_US
dc.subject.meshCell Line, Transformeden_US
dc.subject.meshCercopithecus Aethiopsen_US
dc.subject.meshCulture Techniquesen_US
dc.subject.meshEmbryonic And Fetal Development - Drug Effectsen_US
dc.subject.meshFemaleen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshGrowth Substances - Immunology - Isolation & Purification - Metabolism - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmune Sera - Pharmacologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshOviducts - Chemistryen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshVero Cellsen_US
dc.titleEmbryotrophic factor-3 from human oviductal cells affects the messenger RNA expression of mouse blastocysten_HK
dc.typeArticleen_HK
dc.identifier.emailLee, YL: cherielee@hku.hken_HK
dc.identifier.emailLee, KF: ckflee@hku.hken_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.emailLee, WM: hrszlwm@hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, YL=rp00308en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.identifier.authorityLee, WM=rp00728en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1095/biolreprod.102.007336en_HK
dc.identifier.pmid12533399-
dc.identifier.scopuseid_2-s2.0-0037306796en_HK
dc.identifier.hkuros76880-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037306796&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume68en_HK
dc.identifier.issue2en_HK
dc.identifier.spage375en_HK
dc.identifier.epage382en_HK
dc.identifier.isiWOS:000180644200005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLee, YL=15033851800en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridXu, JS=7408556691en_HK
dc.identifier.scopusauthoridKwok, KL=16645835100en_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK
dc.identifier.scopusauthoridLee, WM=24799156600en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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