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Article: Cdc25B functions as a novel coactivator for the steroid receptors

TitleCdc25B functions as a novel coactivator for the steroid receptors
Authors
Issue Date2001
Citation
Molecular And Cellular Biology, 2001, v. 21 n. 23, p. 8056-8067 How to Cite?
AbstractWe have previously demonstrated that overexpression of Cdc25B in transgenic mice resulted in mammary gland hyperplasia and increased steroid hormone responsiveness. To address how Cdc25B enhances the hormone responsiveness in mammary glands, we showed that Cdc25B stimulates steroid receptor-dependent transcription in transient transfection assays and in a cell-free assay with chromatin templates. Surprisingly, the effect of Cdc25B on steroid receptors is independent of its protein phosphatase activity in vitro. The direct interactions of Cdc25B with steroid receptors, on the other hand, were evidenced in in vivo and in vitro assays, suggesting the potential direct contribution of Cdc25B on the steroid receptor-mediated transcription. In addition, p300/CBP-associated factor and CREB binding protein were shown to interact and synergize with Cdc25B and further enhance its coactivation activity. Thus, we have uncovered a novel function of Cdc25B that serves as a steroid receptor coactivator in addition to its role as a regulator for cell cycle progression. This dual function might likely contribute to its oncogenic action in breast cancer.
Persistent Identifierhttp://hdl.handle.net/10722/172789
ISSN
2015 Impact Factor: 4.427
2015 SCImago Journal Rankings: 3.806
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMa, ZQen_US
dc.contributor.authorLiu, Zen_US
dc.contributor.authorNgan, ESWen_US
dc.contributor.authorTsai, SYen_US
dc.date.accessioned2012-10-30T06:24:57Z-
dc.date.available2012-10-30T06:24:57Z-
dc.date.issued2001en_US
dc.identifier.citationMolecular And Cellular Biology, 2001, v. 21 n. 23, p. 8056-8067en_US
dc.identifier.issn0270-7306en_US
dc.identifier.urihttp://hdl.handle.net/10722/172789-
dc.description.abstractWe have previously demonstrated that overexpression of Cdc25B in transgenic mice resulted in mammary gland hyperplasia and increased steroid hormone responsiveness. To address how Cdc25B enhances the hormone responsiveness in mammary glands, we showed that Cdc25B stimulates steroid receptor-dependent transcription in transient transfection assays and in a cell-free assay with chromatin templates. Surprisingly, the effect of Cdc25B on steroid receptors is independent of its protein phosphatase activity in vitro. The direct interactions of Cdc25B with steroid receptors, on the other hand, were evidenced in in vivo and in vitro assays, suggesting the potential direct contribution of Cdc25B on the steroid receptor-mediated transcription. In addition, p300/CBP-associated factor and CREB binding protein were shown to interact and synergize with Cdc25B and further enhance its coactivation activity. Thus, we have uncovered a novel function of Cdc25B that serves as a steroid receptor coactivator in addition to its role as a regulator for cell cycle progression. This dual function might likely contribute to its oncogenic action in breast cancer.en_US
dc.languageengen_US
dc.relation.ispartofMolecular and Cellular Biologyen_US
dc.subject.meshAcetyltransferases - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBreast - Metabolismen_US
dc.subject.meshCreb-Binding Proteinen_US
dc.subject.meshCell Cycle - Physiologyen_US
dc.subject.meshCell Cycle Proteins - Metabolism - Pharmacologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell-Free System - Metabolismen_US
dc.subject.meshCyclin D1 - Metabolismen_US
dc.subject.meshEnzyme Activation - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation - Physiologyen_US
dc.subject.meshHistone Acetyltransferasesen_US
dc.subject.meshHumansen_US
dc.subject.meshNuclear Proteins - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshReceptors, Androgen - Metabolismen_US
dc.subject.meshReceptors, Estrogen - Metabolismen_US
dc.subject.meshReceptors, Glucocorticoid - Metabolismen_US
dc.subject.meshReceptors, Progesterone - Metabolismen_US
dc.subject.meshReceptors, Steroid - Metabolismen_US
dc.subject.meshSaccharomyces Cerevisiae Proteinsen_US
dc.subject.meshTrans-Activators - Metabolismen_US
dc.subject.meshTranscription Factorsen_US
dc.subject.meshTranscription, Genetic - Drug Effects - Physiologyen_US
dc.subject.meshCdc25 Phosphatases - Metabolism - Pharmacologyen_US
dc.subject.meshP300-Cbp Transcription Factorsen_US
dc.titleCdc25B functions as a novel coactivator for the steroid receptorsen_US
dc.typeArticleen_US
dc.identifier.emailNgan, ESW: engan@hkucc.hku.hken_US
dc.identifier.authorityNgan, ESW=rp00422en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/MCB.21.23.8056-8067.2001en_US
dc.identifier.pmid11689696-
dc.identifier.scopuseid_2-s2.0-0035171497en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035171497&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume21en_US
dc.identifier.issue23en_US
dc.identifier.spage8056en_US
dc.identifier.epage8067en_US
dc.identifier.isiWOS:000172059100016-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMa, ZQ=7403600106en_US
dc.identifier.scopusauthoridLiu, Z=7406677601en_US
dc.identifier.scopusauthoridNgan, ESW=22234827500en_US
dc.identifier.scopusauthoridTsai, SY=7403478781en_US

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