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Article: Specific latent membrane protein 1 gene sequences in type 1 and type 2 Epstein-Barr virus from nasopharyngeal carcinoma in Hong Kong

TitleSpecific latent membrane protein 1 gene sequences in type 1 and type 2 Epstein-Barr virus from nasopharyngeal carcinoma in Hong Kong
Authors
Issue Date1998
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 1998, v. 76 n. 3, p. 399-406 How to Cite?
AbstractWe reported previously that a characteristic Epstein-Barr virus latent membrane protein I (EBV-LMP1) gene was associated with nasopharyngeal carcinoma (NPC) in Hong Kong. It showed a 30 bp deletion at the carboxyl terminus with specific amino acid substitution Asp at codon 335 with reference to Gly in B95-8 LMP1. This deletion variant Asp335 was present in over 90% of NPC biopsy specimens. The present study attempted to determine the whole encoding sequence of the LMP1 gene in different EBV isolates from NPC, and its relation with EBV types. We found that 92% (34/37) of primary NPC tumours harboured EBV-I and possessed the LMP1 deletion variant, of which 86% were Asp335 and 6% were Gly335. EBV-2 was present in 8% (3/37) of tumours and all contained the retention variant of the LMP1 gene. Sequencing of the whole encoding region of the LMP1 gene revealed that the deletion variant Asp335 and deletion variant Gly335 carried similar sequences. They showed 43 common nucleotide substitutions in 41 codons with reference to B95-8. The retention variant showed 52 base changes in 46 codons compared with B95-8. The amino acid alterations in both the deletion and retention variants were mostly clustered at the transmembrane domain of the protein. Furthermore, half of the substitutions were common to both variants, suggesting a common evolutionary selection pressure. Nonetheless, the 2 LMP1 variants showed differences in nucleotide alterations and were associated with different EBV types, suggesting the presence of 2 distinct EBV strains in Hong Kong NPC.
Persistent Identifierhttp://hdl.handle.net/10722/172740
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, STen_US
dc.contributor.authorLeung, SFen_US
dc.contributor.authorLo, KWen_US
dc.contributor.authorChiu, KWen_US
dc.contributor.authorTam, JSLen_US
dc.contributor.authorFok, TFen_US
dc.contributor.authorJohnson, PJen_US
dc.contributor.authorLee, JCKen_US
dc.contributor.authorHuang, DPen_US
dc.date.accessioned2012-10-30T06:24:36Z-
dc.date.available2012-10-30T06:24:36Z-
dc.date.issued1998en_US
dc.identifier.citationInternational Journal Of Cancer, 1998, v. 76 n. 3, p. 399-406en_US
dc.identifier.issn0020-7136en_US
dc.identifier.urihttp://hdl.handle.net/10722/172740-
dc.description.abstractWe reported previously that a characteristic Epstein-Barr virus latent membrane protein I (EBV-LMP1) gene was associated with nasopharyngeal carcinoma (NPC) in Hong Kong. It showed a 30 bp deletion at the carboxyl terminus with specific amino acid substitution Asp at codon 335 with reference to Gly in B95-8 LMP1. This deletion variant Asp335 was present in over 90% of NPC biopsy specimens. The present study attempted to determine the whole encoding sequence of the LMP1 gene in different EBV isolates from NPC, and its relation with EBV types. We found that 92% (34/37) of primary NPC tumours harboured EBV-I and possessed the LMP1 deletion variant, of which 86% were Asp335 and 6% were Gly335. EBV-2 was present in 8% (3/37) of tumours and all contained the retention variant of the LMP1 gene. Sequencing of the whole encoding region of the LMP1 gene revealed that the deletion variant Asp335 and deletion variant Gly335 carried similar sequences. They showed 43 common nucleotide substitutions in 41 codons with reference to B95-8. The retention variant showed 52 base changes in 46 codons compared with B95-8. The amino acid alterations in both the deletion and retention variants were mostly clustered at the transmembrane domain of the protein. Furthermore, half of the substitutions were common to both variants, suggesting a common evolutionary selection pressure. Nonetheless, the 2 LMP1 variants showed differences in nucleotide alterations and were associated with different EBV types, suggesting the presence of 2 distinct EBV strains in Hong Kong NPC.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_US
dc.relation.ispartofInternational Journal of Canceren_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCarcinoma - Complications - Virologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Deletionen_US
dc.subject.meshHerpesviridae Infections - Complicationsen_US
dc.subject.meshHerpesvirus 4, Human - Geneticsen_US
dc.subject.meshHong Kongen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNasopharyngeal Neoplasms - Complications - Virologyen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.subject.meshSequence Homology, Nucleic Aciden_US
dc.subject.meshTumor Virus Infections - Complicationsen_US
dc.subject.meshViral Matrix Proteins - Geneticsen_US
dc.titleSpecific latent membrane protein 1 gene sequences in type 1 and type 2 Epstein-Barr virus from nasopharyngeal carcinoma in Hong Kongen_US
dc.typeArticleen_US
dc.identifier.emailCheung, ST: stcheung@hkucc.hku.hken_US
dc.identifier.authorityCheung, ST=rp00457en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/(SICI)1097-0215(19980504)76:3<399::AID-IJC18>3.0.CO;2-6en_US
dc.identifier.pmid9579578-
dc.identifier.scopuseid_2-s2.0-0031776840en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031776840&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume76en_US
dc.identifier.issue3en_US
dc.identifier.spage399en_US
dc.identifier.epage406en_US
dc.identifier.isiWOS:000073299900018-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridCheung, ST=7202473497en_US
dc.identifier.scopusauthoridLeung, SF=7202044876en_US
dc.identifier.scopusauthoridLo, KW=7402101603en_US
dc.identifier.scopusauthoridChiu, KW=16149416600en_US
dc.identifier.scopusauthoridTam, JSL=24788939600en_US
dc.identifier.scopusauthoridFok, TF=7006455238en_US
dc.identifier.scopusauthoridJohnson, PJ=7405661637en_US
dc.identifier.scopusauthoridLee, JCK=7601456915en_US
dc.identifier.scopusauthoridHuang, DP=7403891486en_US

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