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Article: Pat1: A topoisomerase II-associated protein required for faithful chromosome transmission in Saccharomyces cerevisiae

TitlePat1: A topoisomerase II-associated protein required for faithful chromosome transmission in Saccharomyces cerevisiae
Authors
Issue Date1996
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 1996, v. 24 n. 23, p. 4791-4797 How to Cite?
AbstractSaccharomyces cerevisiae top2 mutants deficient in topoisomerase II activity are defective in chromosome segregation during both mitotic and meiotic cell divisions. To identify proteins that act in concert with topoisomerase II during chromosome segregation in S.cerevisiae, we have used a two-hybrid cloning approach. We report the isolation of the PAT1 gene (for protein associated with topoisomerase II), which encodes a novel 90 kDa proline- and glutamine-rich protein that interacts with a highly conserved, leucine-rich region of topoisomerase II in vivo. Strains lacking Pat1p exhibit a slow growth rate and a phenotype reminiscent of conditional top2 mutants grown at the semi-permissive temperature; most notably, a reduced fidelity of chromosome segregation during both mitosis and meiosis. These findings indicate that the PAT1 gene is necessary for accurate chromosome transmission during cell division in eukaryotic cells and suggest that the interaction of Pat1p and topoisomerase II is an important component of this function.
Persistent Identifierhttp://hdl.handle.net/10722/172721
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Xen_US
dc.contributor.authorWatt, PMen_US
dc.contributor.authorLouis, EJen_US
dc.contributor.authorBoris, RHen_US
dc.contributor.authorHickson, IDen_US
dc.date.accessioned2012-10-30T06:24:29Z-
dc.date.available2012-10-30T06:24:29Z-
dc.date.issued1996en_US
dc.identifier.citationNucleic Acids Research, 1996, v. 24 n. 23, p. 4791-4797en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://hdl.handle.net/10722/172721-
dc.description.abstractSaccharomyces cerevisiae top2 mutants deficient in topoisomerase II activity are defective in chromosome segregation during both mitotic and meiotic cell divisions. To identify proteins that act in concert with topoisomerase II during chromosome segregation in S.cerevisiae, we have used a two-hybrid cloning approach. We report the isolation of the PAT1 gene (for protein associated with topoisomerase II), which encodes a novel 90 kDa proline- and glutamine-rich protein that interacts with a highly conserved, leucine-rich region of topoisomerase II in vivo. Strains lacking Pat1p exhibit a slow growth rate and a phenotype reminiscent of conditional top2 mutants grown at the semi-permissive temperature; most notably, a reduced fidelity of chromosome segregation during both mitosis and meiosis. These findings indicate that the PAT1 gene is necessary for accurate chromosome transmission during cell division in eukaryotic cells and suggest that the interaction of Pat1p and topoisomerase II is an important component of this function.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_US
dc.relation.ispartofNucleic Acids Researchen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshCell Divisionen_US
dc.subject.meshChromosomes, Fungal - Geneticsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna Topoisomerases, Type Ii - Chemistry - Genetics - Metabolismen_US
dc.subject.meshDna, Fungal - Chemistry - Geneticsen_US
dc.subject.meshDna-Binding Proteins - Genetics - Metabolismen_US
dc.subject.meshGene Deletionen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshLeucineen_US
dc.subject.meshMeiosisen_US
dc.subject.meshMitosisen_US
dc.subject.meshMutationen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshRna-Binding Proteinsen_US
dc.subject.meshRecombinant Proteinsen_US
dc.subject.meshSaccharomyces Cerevisiae - Cytology - Genetics - Growth & Developmenten_US
dc.subject.meshSaccharomyces Cerevisiae Proteinsen_US
dc.titlePat1: A topoisomerase II-associated protein required for faithful chromosome transmission in Saccharomyces cerevisiaeen_US
dc.typeArticleen_US
dc.identifier.emailWang, X: xqwang@hkucc.hku.hken_US
dc.identifier.authorityWang, X=rp00507en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/nar/24.23.4791-
dc.identifier.pmid8972867-
dc.identifier.scopuseid_2-s2.0-0029804024en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029804024&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume24en_US
dc.identifier.issue23en_US
dc.identifier.spage4791en_US
dc.identifier.epage4797en_US
dc.identifier.isiWOS:A1996VY19800025-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridWang, X=17343159900en_US
dc.identifier.scopusauthoridWatt, PM=7103201711en_US
dc.identifier.scopusauthoridLouis, EJ=7202755363en_US
dc.identifier.scopusauthoridBoris, RH=6507613528en_US
dc.identifier.scopusauthoridHickson, ID=7005618389en_US

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