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Article: Comparison of three stool-processing methods for detection of Salmonella serogroups B, C2, and D by PCR

TitleComparison of three stool-processing methods for detection of Salmonella serogroups B, C2, and D by PCR
Authors
Kongmuang, ULuk, JMCLindberg, AA
Issue Date1994
Citation
Journal Of Clinical Microbiology, 1994, v. 32 n. 12, p. 3072-3074 How to Cite?
DOI: http://dx.doi.org/10.1128/JCM.32.12.3072-3074.1994
AbstractThree different stool sample-processing methods (centrifugation, immunomagnetic separation, and selective enrichment cultivation) for the identification of Salmonella serogroups by PCR were studied. The corresponding sensitivities in an ethidium bromide stained-agarose gel were 105, 103, and 10 bacteria, respectively. The PCR assay with overnight enrichment performed as well as, or even better than, the conventional culture technique. Of 485 clinical stool samples, PCR correctly identified all 230 culture-positive samples as well as mixed Salmonella infections in four cases.
Persistent Identifierhttp://hdl.handle.net/10722/172701
ISSN
0095-1137
2021 Impact Factor: 11.677
2020 SCImago Journal Rankings: 2.349
ISI Accession Number ID
WOS:A1994PT18700038

 

DC FieldValueLanguage
dc.contributor.authorKongmuang, Uen_US
dc.contributor.authorLuk, JMCen_US
dc.contributor.authorLindberg, AAen_US
dc.date.accessioned2012-10-30T06:24:22Z-
dc.date.available2012-10-30T06:24:22Z-
dc.date.issued1994en_US
dc.identifier.citationJournal Of Clinical Microbiology, 1994, v. 32 n. 12, p. 3072-3074en_US
dc.identifier.issn0095-1137en_US
dc.identifier.urihttp://hdl.handle.net/10722/172701-
dc.description.abstractThree different stool sample-processing methods (centrifugation, immunomagnetic separation, and selective enrichment cultivation) for the identification of Salmonella serogroups by PCR were studied. The corresponding sensitivities in an ethidium bromide stained-agarose gel were 105, 103, and 10 bacteria, respectively. The PCR assay with overnight enrichment performed as well as, or even better than, the conventional culture technique. Of 485 clinical stool samples, PCR correctly identified all 230 culture-positive samples as well as mixed Salmonella infections in four cases.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.subject.meshBacteriological Techniquesen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshDna, Bacterial - Analysisen_US
dc.subject.meshFeces - Microbiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshSalmonella - Classification - Genetics - Isolation & Purificationen_US
dc.subject.meshSalmonella Infections - Diagnosisen_US
dc.titleComparison of three stool-processing methods for detection of Salmonella serogroups B, C2, and D by PCRen_US
dc.typeArticleen_US
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_US
dc.identifier.authorityLuk, JMC=rp00349en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/JCM.32.12.3072-3074.1994-
dc.identifier.pmid7883906-
dc.identifier.scopuseid_2-s2.0-0027959935en_US
dc.identifier.volume32en_US
dc.identifier.issue12en_US
dc.identifier.spage3072en_US
dc.identifier.epage3074en_US
dc.identifier.isiWOS:A1994PT18700038-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKongmuang, U=6602328523en_US
dc.identifier.scopusauthoridLuk, JMC=7006777791en_US
dc.identifier.scopusauthoridLindberg, AA=7101776377en_US
dc.identifier.issnl0095-1137-

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