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Article: Selective amplification of abequose and paratose synthase genes (rfb) by polymerase chain reaction for identification of Salmonella major serogroups (A, B, C2, and D)

TitleSelective amplification of abequose and paratose synthase genes (rfb) by polymerase chain reaction for identification of Salmonella major serogroups (A, B, C2, and D)
Authors
Issue Date1993
Citation
Journal Of Clinical Microbiology, 1993, v. 31 n. 8, p. 2118-2123 How to Cite?
AbstractMany parts of the Salmonella rfb gene clusters which are responsible for biosynthesis of the oligosaccharide-repeating units of the O-antigenic lipopolysaccharide have recently been cloned and sequenced. On the basis of this knowledge, three sets of nucleotide primers were selected to target defined regions of the abequose and paratose synthase genes: rfbJ of Salmonella serogroup B, rfbJ of Salmonella serogroup C2, and rfbS of Salmonella serogroup D (also present in serogroup A). For good differentiation among these major serogroups, the primers were designed not only to give precise specificity in priming but also to give DNA products with different sizes in polymerase chain reactions (product sizes, ~720 bp for both serogroups A and D, ~820 bp for serogroup C2, and ~882 bp for serogroup B). In a polymerase chain reaction assay utilizing these rfb- specific primers, all of the 40 salmonellae belonging to serogroups B, C2, and D plus A were accurately identified among a total of 123 clinical isolates tested (including 55 salmonellae from 36 different serotypes and 68 strains from 10 other members of the family Enterobacteriaceae). No false- positive reactions were detected. The selected rfb gene sequences were proved for the first time to be useful DNA-based markers for identification of and differentiation among Salmonella serogroups A, B, C2, and D.
Persistent Identifierhttp://hdl.handle.net/10722/172687
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLuk, JMCen_US
dc.contributor.authorKongmuang, Uen_US
dc.contributor.authorReeves, PRen_US
dc.contributor.authorLindberg, AAen_US
dc.date.accessioned2012-10-30T06:24:17Z-
dc.date.available2012-10-30T06:24:17Z-
dc.date.issued1993en_US
dc.identifier.citationJournal Of Clinical Microbiology, 1993, v. 31 n. 8, p. 2118-2123en_US
dc.identifier.issn0095-1137en_US
dc.identifier.urihttp://hdl.handle.net/10722/172687-
dc.description.abstractMany parts of the Salmonella rfb gene clusters which are responsible for biosynthesis of the oligosaccharide-repeating units of the O-antigenic lipopolysaccharide have recently been cloned and sequenced. On the basis of this knowledge, three sets of nucleotide primers were selected to target defined regions of the abequose and paratose synthase genes: rfbJ of Salmonella serogroup B, rfbJ of Salmonella serogroup C2, and rfbS of Salmonella serogroup D (also present in serogroup A). For good differentiation among these major serogroups, the primers were designed not only to give precise specificity in priming but also to give DNA products with different sizes in polymerase chain reactions (product sizes, ~720 bp for both serogroups A and D, ~820 bp for serogroup C2, and ~882 bp for serogroup B). In a polymerase chain reaction assay utilizing these rfb- specific primers, all of the 40 salmonellae belonging to serogroups B, C2, and D plus A were accurately identified among a total of 123 clinical isolates tested (including 55 salmonellae from 36 different serotypes and 68 strains from 10 other members of the family Enterobacteriaceae). No false- positive reactions were detected. The selected rfb gene sequences were proved for the first time to be useful DNA-based markers for identification of and differentiation among Salmonella serogroups A, B, C2, and D.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCarbohydrate Epimerases - Geneticsen_US
dc.subject.meshDna, Bacterial - Chemistryen_US
dc.subject.meshGenes, Bacterialen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMultigene Familyen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshSalmonella - Classification - Enzymology - Genetics - Isolation & Purificationen_US
dc.subject.meshSerotypingen_US
dc.titleSelective amplification of abequose and paratose synthase genes (rfb) by polymerase chain reaction for identification of Salmonella major serogroups (A, B, C2, and D)en_US
dc.typeArticleen_US
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_US
dc.identifier.authorityLuk, JMC=rp00349en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8370740-
dc.identifier.scopuseid_2-s2.0-0027202076en_US
dc.identifier.volume31en_US
dc.identifier.issue8en_US
dc.identifier.spage2118en_US
dc.identifier.epage2123en_US
dc.identifier.isiWOS:A1993LM79500030-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLuk, JMC=7006777791en_US
dc.identifier.scopusauthoridKongmuang, U=6602328523en_US
dc.identifier.scopusauthoridReeves, PR=7102295636en_US
dc.identifier.scopusauthoridLindberg, AA=7101776377en_US

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