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Article: Detection of enterobacterial lipopolysaccharides and experimental endotoxemia by means of an immunolimulus assay using both serotype-specific and cross-reactive antibodies

TitleDetection of enterobacterial lipopolysaccharides and experimental endotoxemia by means of an immunolimulus assay using both serotype-specific and cross-reactive antibodies
Authors
Issue Date1993
PublisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org
Citation
Journal Of Infectious Diseases, 1993, v. 168 n. 2, p. 393-399 How to Cite?
AbstractThe immunolimulus (IML) assay system uses solid-phase endotoxin antibodies to capture lipopolysaccharide (LPS), which is then quantified by a modification of the chromogenic limulus amebocyte lysate (CLAL) method. Monoclonal antibodies (MAbs) reactive with selected O antigen serotypes of Escherichia coli (O18) and Salmonella typhimurium (O-9,12), when used in the IML, were shown to be highly specific in detecting their respective endotoxins in purified form and in plasma samples from experimentally infected animals. A murine MAb that was broadly cross-reactive with E. coli, Salmonella, and Shigella endotoxins also proved to be highly effective in the IML assay for capturing LPS molecules from both E. coli and S. typhimurium strains. These results indicate that IML assays can detect smooth-type enterobacterial endotoxins in plasma and suggest that such assays have potential for use in the rapid diagnosis of sepsis and endotoxemia caused by different enterobacterial species.
Persistent Identifierhttp://hdl.handle.net/10722/172685
ISSN
2015 Impact Factor: 6.344
2015 SCImago Journal Rankings: 4.000
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSaxen, Hen_US
dc.contributor.authorVuopioVarkila, Jen_US
dc.contributor.authorLuk, Jen_US
dc.contributor.authorLindberg, Aen_US
dc.contributor.authorLang, Aen_US
dc.contributor.authorDi Padova, Fen_US
dc.contributor.authorCryz Jr, SJen_US
dc.contributor.authorMertsola, Jen_US
dc.contributor.authorMccracken Jr, GHen_US
dc.contributor.authorHansen, EJen_US
dc.date.accessioned2012-10-30T06:24:16Z-
dc.date.available2012-10-30T06:24:16Z-
dc.date.issued1993en_US
dc.identifier.citationJournal Of Infectious Diseases, 1993, v. 168 n. 2, p. 393-399en_US
dc.identifier.issn0022-1899en_US
dc.identifier.urihttp://hdl.handle.net/10722/172685-
dc.description.abstractThe immunolimulus (IML) assay system uses solid-phase endotoxin antibodies to capture lipopolysaccharide (LPS), which is then quantified by a modification of the chromogenic limulus amebocyte lysate (CLAL) method. Monoclonal antibodies (MAbs) reactive with selected O antigen serotypes of Escherichia coli (O18) and Salmonella typhimurium (O-9,12), when used in the IML, were shown to be highly specific in detecting their respective endotoxins in purified form and in plasma samples from experimentally infected animals. A murine MAb that was broadly cross-reactive with E. coli, Salmonella, and Shigella endotoxins also proved to be highly effective in the IML assay for capturing LPS molecules from both E. coli and S. typhimurium strains. These results indicate that IML assays can detect smooth-type enterobacterial endotoxins in plasma and suggest that such assays have potential for use in the rapid diagnosis of sepsis and endotoxemia caused by different enterobacterial species.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.orgen_US
dc.relation.ispartofJournal of Infectious Diseasesen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshAntibodies, Bacterial - Immunologyen_US
dc.subject.meshAntibodies, Monoclonal - Immunologyen_US
dc.subject.meshCross Reactionsen_US
dc.subject.meshEndotoxins - Blooden_US
dc.subject.meshEnterobacteriaceae - Chemistryen_US
dc.subject.meshEscherichia Coli Infections - Blooden_US
dc.subject.meshHumansen_US
dc.subject.meshInfant, Newbornen_US
dc.subject.meshLimulus Testen_US
dc.subject.meshLipopolysaccharides - Analysisen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshMice, Inbred Cbaen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSerotypingen_US
dc.subject.meshToxemia - Blooden_US
dc.titleDetection of enterobacterial lipopolysaccharides and experimental endotoxemia by means of an immunolimulus assay using both serotype-specific and cross-reactive antibodiesen_US
dc.typeArticleen_US
dc.identifier.emailLuk, J: jmluk@hkucc.hku.hken_US
dc.identifier.authorityLuk, J=rp00349en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/infdis/168.2.393-
dc.identifier.pmid8335976-
dc.identifier.scopuseid_2-s2.0-0027172218en_US
dc.identifier.volume168en_US
dc.identifier.issue2en_US
dc.identifier.spage393en_US
dc.identifier.epage399en_US
dc.identifier.isiWOS:A1993LN81300020-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSaxen, H=7006630985en_US
dc.identifier.scopusauthoridVuopioVarkila, J=7003419423en_US
dc.identifier.scopusauthoridLuk, J=7006777791en_US
dc.identifier.scopusauthoridLindberg, A=7101776377en_US
dc.identifier.scopusauthoridLang, A=25934609800en_US
dc.identifier.scopusauthoridDi Padova, F=7006475044en_US
dc.identifier.scopusauthoridCryz Jr, SJ=7006814360en_US
dc.identifier.scopusauthoridMertsola, J=7004911408en_US
dc.identifier.scopusauthoridMcCracken Jr, GH=35459584100en_US
dc.identifier.scopusauthoridHansen, EJ=7402448403en_US

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