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Article: Epitope mapping of four monoclonal antibodies recognizing the hexose core domain of Salmonella lipopolysaccharide

TitleEpitope mapping of four monoclonal antibodies recognizing the hexose core domain of Salmonella lipopolysaccharide
Authors
Issue Date1991
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1991, v. 266 n. 34, p. 23215-23225 How to Cite?
AbstractFour murine monoclonal antibodies reactive with distinctive regions of the hexose core domain of Salmonella lipopolysaccharide (LPS) were generated and their epitope specificities were delineated. MAST 56 (IgG1) and MAST 50 (IgG3) antibodies elicited by immunizations with Salmonella typhimurium Rb1 and Rb2 mutants, reacted selectively in enzyme immunoassay with the LPS from rough mutants. In contrast, MATy 1 (IgM) and MATy 2 (IgG2b) antibodies raised by an attenuated Salmonella typhi 620 Ty strain were reactive with LPS from both smooth and rough Salmonellae. Immunoblotting analysis showed that MATy 1 distinguished only the bottom bands (naked LPS core) among the heterogeneous LPS populations, whereas MATy 2 gave a ladder pattern (reactive with both naked and O-chain-substituted LPS cores). Differential binding specificaties of MATy 1 and MATy2 antibodies to the naked and capped LPS cores were further analyzed utilizing S. typhimurium polysaccharide fractions with different O-chain:core ratios which were obtained after separation by Sephacryl S-200 chromatography. Steric effects on the antibody reactivity by the bulky O-polysaccharide chain were detected. The use of chemically defined native and synthetic saccharides as inhibitors, in combination with the conformation of the Salmonella core oligosaccharide, permitted the definition of antigenic determinants carried in the core domain recognized by each antibody: (i) the branches I and VIII are essential for MATy 1 recognition, (ii) the backbone III-IV-V for MATy 2, (iii) the backbone II-III-IV-V for MAST 56, and (iv) the backbone plus the branch III-IV-V-VIII for MAST 50.
Persistent Identifierhttp://hdl.handle.net/10722/172659
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLuk, JMCen_US
dc.contributor.authorLind, SMen_US
dc.contributor.authorTsang, RSWen_US
dc.contributor.authorLindberg, AAen_US
dc.date.accessioned2012-10-30T06:24:05Z-
dc.date.available2012-10-30T06:24:05Z-
dc.date.issued1991en_US
dc.identifier.citationJournal Of Biological Chemistry, 1991, v. 266 n. 34, p. 23215-23225en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/172659-
dc.description.abstractFour murine monoclonal antibodies reactive with distinctive regions of the hexose core domain of Salmonella lipopolysaccharide (LPS) were generated and their epitope specificities were delineated. MAST 56 (IgG1) and MAST 50 (IgG3) antibodies elicited by immunizations with Salmonella typhimurium Rb1 and Rb2 mutants, reacted selectively in enzyme immunoassay with the LPS from rough mutants. In contrast, MATy 1 (IgM) and MATy 2 (IgG2b) antibodies raised by an attenuated Salmonella typhi 620 Ty strain were reactive with LPS from both smooth and rough Salmonellae. Immunoblotting analysis showed that MATy 1 distinguished only the bottom bands (naked LPS core) among the heterogeneous LPS populations, whereas MATy 2 gave a ladder pattern (reactive with both naked and O-chain-substituted LPS cores). Differential binding specificaties of MATy 1 and MATy2 antibodies to the naked and capped LPS cores were further analyzed utilizing S. typhimurium polysaccharide fractions with different O-chain:core ratios which were obtained after separation by Sephacryl S-200 chromatography. Steric effects on the antibody reactivity by the bulky O-polysaccharide chain were detected. The use of chemically defined native and synthetic saccharides as inhibitors, in combination with the conformation of the Salmonella core oligosaccharide, permitted the definition of antigenic determinants carried in the core domain recognized by each antibody: (i) the branches I and VIII are essential for MATy 1 recognition, (ii) the backbone III-IV-V for MATy 2, (iii) the backbone II-III-IV-V for MAST 56, and (iv) the backbone plus the branch III-IV-V-VIII for MAST 50.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshAntibodies, Bacterial - Immunologyen_US
dc.subject.meshAntibodies, Monoclonal - Immunologyen_US
dc.subject.meshAntibody Specificityen_US
dc.subject.meshCarbohydrate Sequenceen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshEpitopesen_US
dc.subject.meshHexoses - Immunologyen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshImmunoglobulin G - Immunologyen_US
dc.subject.meshLipopolysaccharides - Chemistry - Immunologyen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMolecular Structureen_US
dc.subject.meshSalmonella Typhimurium - Immunologyen_US
dc.titleEpitope mapping of four monoclonal antibodies recognizing the hexose core domain of Salmonella lipopolysaccharideen_US
dc.typeArticleen_US
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_US
dc.identifier.authorityLuk, JMC=rp00349en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1720777-
dc.identifier.scopuseid_2-s2.0-0026334366en_US
dc.identifier.volume266en_US
dc.identifier.issue34en_US
dc.identifier.spage23215en_US
dc.identifier.epage23225en_US
dc.identifier.isiWOS:A1991GT48300068-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLuk, JMC=7006777791en_US
dc.identifier.scopusauthoridLind, SM=7005241716en_US
dc.identifier.scopusauthoridTsang, RSW=7102940066en_US
dc.identifier.scopusauthoridLindberg, AA=7101776377en_US

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