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- Publisher Website: 10.1016/0022-1759(91)90387-U
- Scopus: eid_2-s2.0-0026034328
- PMID: 1707080
- WOS: WOS:A1991FB24000001
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Article: Rapid and sensitive detection of Salmonella (O:6,7) by immunomagnetic monoclonal antibody-based assays
Title | Rapid and sensitive detection of Salmonella (O:6,7) by immunomagnetic monoclonal antibody-based assays |
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Authors | |
Keywords | Enzyme immunoassay Magnetic bead Monoclonal antibody Salmonella O:6,7 lipopolysaccharide |
Issue Date | 1991 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim |
Citation | Journal Of Immunological Methods, 1991, v. 137 n. 1, p. 1-8 How to Cite? |
Abstract | An immunomagnetic technique to detect and identify Salmonella serogroup C1 has been developed. Monoclonal antibodies (MAbs) specific for the O antigen 6,7 of Salmonella lipopolysaccharide (LPS) coupled to magnetic beads were used to isolate the salmonellae. Captured bacteria were easily identified by acridine orange staining and measured by enzyme immunoassays with a conjugate anti-LPS MAb as the detector probe. The whole detection process required 2-3 h and the sensitivity was 103-104 bacteria/ml. The presence of blood (10%, v/v) or stool (1%, w/v) components did not interfere with the immunomagnetic assay performance. |
Persistent Identifier | http://hdl.handle.net/10722/172655 |
ISSN | 2023 Impact Factor: 1.6 2023 SCImago Journal Rankings: 0.555 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Luk, JMC | en_US |
dc.contributor.author | Lindberg, AA | en_US |
dc.date.accessioned | 2012-10-30T06:24:03Z | - |
dc.date.available | 2012-10-30T06:24:03Z | - |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | Journal Of Immunological Methods, 1991, v. 137 n. 1, p. 1-8 | en_US |
dc.identifier.issn | 0022-1759 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/172655 | - |
dc.description.abstract | An immunomagnetic technique to detect and identify Salmonella serogroup C1 has been developed. Monoclonal antibodies (MAbs) specific for the O antigen 6,7 of Salmonella lipopolysaccharide (LPS) coupled to magnetic beads were used to isolate the salmonellae. Captured bacteria were easily identified by acridine orange staining and measured by enzyme immunoassays with a conjugate anti-LPS MAb as the detector probe. The whole detection process required 2-3 h and the sensitivity was 103-104 bacteria/ml. The presence of blood (10%, v/v) or stool (1%, w/v) components did not interfere with the immunomagnetic assay performance. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim | en_US |
dc.relation.ispartof | Journal of Immunological Methods | en_US |
dc.subject | Enzyme immunoassay | - |
dc.subject | Magnetic bead | - |
dc.subject | Monoclonal antibody | - |
dc.subject | Salmonella O:6,7 lipopolysaccharide | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibodies, Monoclonal - Diagnostic Use | en_US |
dc.subject.mesh | Antigens, Bacterial - Analysis - Immunology | en_US |
dc.subject.mesh | Feces - Microbiology | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Immunoenzyme Techniques | en_US |
dc.subject.mesh | Latex Fixation Tests | en_US |
dc.subject.mesh | Magnetics | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | O Antigens | en_US |
dc.subject.mesh | Salmonella - Immunology - Isolation & Purification | en_US |
dc.title | Rapid and sensitive detection of Salmonella (O:6,7) by immunomagnetic monoclonal antibody-based assays | en_US |
dc.type | Article | en_US |
dc.identifier.email | Luk, JMC: jmluk@hkucc.hku.hk | en_US |
dc.identifier.authority | Luk, JMC=rp00349 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/0022-1759(91)90387-U | - |
dc.identifier.pmid | 1707080 | - |
dc.identifier.scopus | eid_2-s2.0-0026034328 | en_US |
dc.identifier.volume | 137 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 1 | en_US |
dc.identifier.epage | 8 | en_US |
dc.identifier.isi | WOS:A1991FB24000001 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Luk, JMC=7006777791 | en_US |
dc.identifier.scopusauthorid | Lindberg, AA=7101776377 | en_US |
dc.identifier.issnl | 0022-1759 | - |