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- Publisher Website: 10.1128/JCM.29.1.120-130.1991
- Scopus: eid_2-s2.0-0026032734
- PMID: 1993746
- WOS: WOS:A1991EP04400025
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Article: Evaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria
Title | Evaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria |
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Authors | |
Issue Date | 1991 |
Citation | Journal Of Clinical Microbiology, 1991, v. 29 n. 1, p. 120-130 How to Cite? |
Abstract | After experimental conditions were established, 366 strains of mycobacteria belonging to 23 different species were studied for fatty acids, secondary alcohols, and mycolic acids cleavage products by capillary gas-liquid chromatography. Additionally, the mycolic acid pattern was studied by thin-layer chromatography. Capillary gas-liquid chromatography allowed direct identification of the following Mycobacterium spp.: M. kansasii, M. marinum, M. szulgai, M. xenopi, M. malmoense, and M. gordonae. The patterns of mycolic acid methyl esters recorded for the test strains of M. chelonae and M. agri may be of value in the identification of these species. Moreover, the combined use of the two chromatographic techniques provided precise identification of the M. tuberculosis complex, M. simiae, M. fallax, M. triviaele, and M. chelonae-like organisms. A minimal set of biochemical tests is usually required to obtain identification to the species level when chromatographic procedures alone are not sufficient. Under the reported experimental conditions, thin-layer chromatography and capillary gas-liquid chromatography are rapid and very useful techniques for the identification of mycobacteria. |
Persistent Identifier | http://hdl.handle.net/10722/172654 |
ISSN | 2023 Impact Factor: 6.1 2023 SCImago Journal Rankings: 1.653 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Luquin, M | en_US |
dc.contributor.author | Ausina, V | en_US |
dc.contributor.author | Lopez Calahorra, F | en_US |
dc.contributor.author | Belda, F | en_US |
dc.contributor.author | Barcelo, MG | en_US |
dc.contributor.author | Celma, C | en_US |
dc.contributor.author | Prats, G | en_US |
dc.date.accessioned | 2012-10-30T06:24:03Z | - |
dc.date.available | 2012-10-30T06:24:03Z | - |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | Journal Of Clinical Microbiology, 1991, v. 29 n. 1, p. 120-130 | en_US |
dc.identifier.issn | 0095-1137 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/172654 | - |
dc.description.abstract | After experimental conditions were established, 366 strains of mycobacteria belonging to 23 different species were studied for fatty acids, secondary alcohols, and mycolic acids cleavage products by capillary gas-liquid chromatography. Additionally, the mycolic acid pattern was studied by thin-layer chromatography. Capillary gas-liquid chromatography allowed direct identification of the following Mycobacterium spp.: M. kansasii, M. marinum, M. szulgai, M. xenopi, M. malmoense, and M. gordonae. The patterns of mycolic acid methyl esters recorded for the test strains of M. chelonae and M. agri may be of value in the identification of these species. Moreover, the combined use of the two chromatographic techniques provided precise identification of the M. tuberculosis complex, M. simiae, M. fallax, M. triviaele, and M. chelonae-like organisms. A minimal set of biochemical tests is usually required to obtain identification to the species level when chromatographic procedures alone are not sufficient. Under the reported experimental conditions, thin-layer chromatography and capillary gas-liquid chromatography are rapid and very useful techniques for the identification of mycobacteria. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Journal of Clinical Microbiology | en_US |
dc.subject.mesh | Alcohols - Analysis | en_US |
dc.subject.mesh | Chromatography, Gas - Methods | en_US |
dc.subject.mesh | Chromatography, Thin Layer - Methods | en_US |
dc.subject.mesh | Evaluation Studies As Topic | en_US |
dc.subject.mesh | Fatty Acids - Analysis | en_US |
dc.subject.mesh | Mycobacterium - Classification - Growth & Development | en_US |
dc.subject.mesh | Mycolic Acids - Analysis | en_US |
dc.subject.mesh | Reproducibility Of Results | en_US |
dc.subject.mesh | Species Specificity | en_US |
dc.title | Evaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria | en_US |
dc.type | Article | en_US |
dc.identifier.email | Barcelo, MG: mmgarcia@hkucc.hku.hk | en_US |
dc.identifier.authority | Barcelo, MG=rp00445 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1128/JCM.29.1.120-130.1991 | - |
dc.identifier.pmid | 1993746 | - |
dc.identifier.scopus | eid_2-s2.0-0026032734 | en_US |
dc.identifier.volume | 29 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 120 | en_US |
dc.identifier.epage | 130 | en_US |
dc.identifier.isi | WOS:A1991EP04400025 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Luquin, M=7006736171 | en_US |
dc.identifier.scopusauthorid | Ausina, V=35394134800 | en_US |
dc.identifier.scopusauthorid | Lopez Calahorra, F=16208118000 | en_US |
dc.identifier.scopusauthorid | Belda, F=7006412311 | en_US |
dc.identifier.scopusauthorid | Barcelo, MG=6701767303 | en_US |
dc.identifier.scopusauthorid | Celma, C=6602838101 | en_US |
dc.identifier.scopusauthorid | Prats, G=35396313500 | en_US |
dc.identifier.issnl | 0095-1137 | - |