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Article: Evaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria

TitleEvaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria
Authors
Issue Date1991
Citation
Journal Of Clinical Microbiology, 1991, v. 29 n. 1, p. 120-130 How to Cite?
AbstractAfter experimental conditions were established, 366 strains of mycobacteria belonging to 23 different species were studied for fatty acids, secondary alcohols, and mycolic acids cleavage products by capillary gas-liquid chromatography. Additionally, the mycolic acid pattern was studied by thin-layer chromatography. Capillary gas-liquid chromatography allowed direct identification of the following Mycobacterium spp.: M. kansasii, M. marinum, M. szulgai, M. xenopi, M. malmoense, and M. gordonae. The patterns of mycolic acid methyl esters recorded for the test strains of M. chelonae and M. agri may be of value in the identification of these species. Moreover, the combined use of the two chromatographic techniques provided precise identification of the M. tuberculosis complex, M. simiae, M. fallax, M. triviaele, and M. chelonae-like organisms. A minimal set of biochemical tests is usually required to obtain identification to the species level when chromatographic procedures alone are not sufficient. Under the reported experimental conditions, thin-layer chromatography and capillary gas-liquid chromatography are rapid and very useful techniques for the identification of mycobacteria.
Persistent Identifierhttp://hdl.handle.net/10722/172654
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLuquin, Men_US
dc.contributor.authorAusina, Ven_US
dc.contributor.authorLopez Calahorra, Fen_US
dc.contributor.authorBelda, Fen_US
dc.contributor.authorBarcelo, MGen_US
dc.contributor.authorCelma, Cen_US
dc.contributor.authorPrats, Gen_US
dc.date.accessioned2012-10-30T06:24:03Z-
dc.date.available2012-10-30T06:24:03Z-
dc.date.issued1991en_US
dc.identifier.citationJournal Of Clinical Microbiology, 1991, v. 29 n. 1, p. 120-130en_US
dc.identifier.issn0095-1137en_US
dc.identifier.urihttp://hdl.handle.net/10722/172654-
dc.description.abstractAfter experimental conditions were established, 366 strains of mycobacteria belonging to 23 different species were studied for fatty acids, secondary alcohols, and mycolic acids cleavage products by capillary gas-liquid chromatography. Additionally, the mycolic acid pattern was studied by thin-layer chromatography. Capillary gas-liquid chromatography allowed direct identification of the following Mycobacterium spp.: M. kansasii, M. marinum, M. szulgai, M. xenopi, M. malmoense, and M. gordonae. The patterns of mycolic acid methyl esters recorded for the test strains of M. chelonae and M. agri may be of value in the identification of these species. Moreover, the combined use of the two chromatographic techniques provided precise identification of the M. tuberculosis complex, M. simiae, M. fallax, M. triviaele, and M. chelonae-like organisms. A minimal set of biochemical tests is usually required to obtain identification to the species level when chromatographic procedures alone are not sufficient. Under the reported experimental conditions, thin-layer chromatography and capillary gas-liquid chromatography are rapid and very useful techniques for the identification of mycobacteria.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.subject.meshAlcohols - Analysisen_US
dc.subject.meshChromatography, Gas - Methodsen_US
dc.subject.meshChromatography, Thin Layer - Methodsen_US
dc.subject.meshEvaluation Studies As Topicen_US
dc.subject.meshFatty Acids - Analysisen_US
dc.subject.meshMycobacterium - Classification - Growth & Developmenten_US
dc.subject.meshMycolic Acids - Analysisen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshSpecies Specificityen_US
dc.titleEvaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteriaen_US
dc.typeArticleen_US
dc.identifier.emailBarcelo, MG: mmgarcia@hkucc.hku.hken_US
dc.identifier.authorityBarcelo, MG=rp00445en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1993746-
dc.identifier.scopuseid_2-s2.0-0026032734en_US
dc.identifier.volume29en_US
dc.identifier.issue1en_US
dc.identifier.spage120en_US
dc.identifier.epage130en_US
dc.identifier.isiWOS:A1991EP04400025-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLuquin, M=7006736171en_US
dc.identifier.scopusauthoridAusina, V=35394134800en_US
dc.identifier.scopusauthoridLopez Calahorra, F=16208118000en_US
dc.identifier.scopusauthoridBelda, F=7006412311en_US
dc.identifier.scopusauthoridBarcelo, MG=6701767303en_US
dc.identifier.scopusauthoridCelma, C=6602838101en_US
dc.identifier.scopusauthoridPrats, G=35396313500en_US

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