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Article: Anti-Salmonella lipopolysaccharide monoclonal antibodies: Characterization of Salmonella BO-, CO-, DO-, and EO-specific clones and their diagnostic usefulness

TitleAnti-Salmonella lipopolysaccharide monoclonal antibodies: Characterization of Salmonella BO-, CO-, DO-, and EO-specific clones and their diagnostic usefulness
Authors
Issue Date1991
Citation
Journal Of Clinical Microbiology, 1991, v. 29 n. 11, p. 2424-2433 How to Cite?
AbstractTo facilitate the identification and serotyping of Salmonella species, we established a wide variety of murine monoclonal antibodies (MAbs) that were reactive with the lipopolysaccharides (LPSs) of Salmonella serogroups B to E. An effective approach for generating LPS-reactive hybridomas was used; this required immunization of mice with LPS-coated bacteria. To screen for diagnostically useful MAbs, the MAbs were tested by enzyme-linked immunosorbent assay against a set of purified LPSs from smooth and rough Salmonella strains. At least four major groups of antibody specificities were identified: Salmonella (i) BO specific, (ii) CO specific, (iii) DO specific, and (iv) EO specific. For a more detailed epitope analysis, a panel of eight different serogroup-specific MAbs which were shown to bind the O-antigenic polysaccharide chains, yielding characteristic ladder patterns in Western blots (immunoblots) against the LPS of Salmonella serogroups B to E, were selected. The availability of various chemically defined LPS structures and Salmonella O-antigen glycoconjugates permitted the definition of O-antigenic polysaccharide epitopes recognized by each MAb that serologically corresponded to factors O3, O4, O5, O6, O7, O8, O9, and O10 on the basis of the Kauffmann-White scheme for Salmonella classification. The diagnostic accuracy of these immunochemically defined O-specific MAbs for Salmonella serotyping was demonstrated by correct identification of all 167 salmonellae (including 72 serotypes from serogroups B to E) among the 294 bacterial strains in a slide agglutination test. No false-positive reactions were detected.
Persistent Identifierhttp://hdl.handle.net/10722/172652
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLuk, JMCen_US
dc.contributor.authorLindberg, AAen_US
dc.date.accessioned2012-10-30T06:24:02Z-
dc.date.available2012-10-30T06:24:02Z-
dc.date.issued1991en_US
dc.identifier.citationJournal Of Clinical Microbiology, 1991, v. 29 n. 11, p. 2424-2433en_US
dc.identifier.issn0095-1137en_US
dc.identifier.urihttp://hdl.handle.net/10722/172652-
dc.description.abstractTo facilitate the identification and serotyping of Salmonella species, we established a wide variety of murine monoclonal antibodies (MAbs) that were reactive with the lipopolysaccharides (LPSs) of Salmonella serogroups B to E. An effective approach for generating LPS-reactive hybridomas was used; this required immunization of mice with LPS-coated bacteria. To screen for diagnostically useful MAbs, the MAbs were tested by enzyme-linked immunosorbent assay against a set of purified LPSs from smooth and rough Salmonella strains. At least four major groups of antibody specificities were identified: Salmonella (i) BO specific, (ii) CO specific, (iii) DO specific, and (iv) EO specific. For a more detailed epitope analysis, a panel of eight different serogroup-specific MAbs which were shown to bind the O-antigenic polysaccharide chains, yielding characteristic ladder patterns in Western blots (immunoblots) against the LPS of Salmonella serogroups B to E, were selected. The availability of various chemically defined LPS structures and Salmonella O-antigen glycoconjugates permitted the definition of O-antigenic polysaccharide epitopes recognized by each MAb that serologically corresponded to factors O3, O4, O5, O6, O7, O8, O9, and O10 on the basis of the Kauffmann-White scheme for Salmonella classification. The diagnostic accuracy of these immunochemically defined O-specific MAbs for Salmonella serotyping was demonstrated by correct identification of all 167 salmonellae (including 72 serotypes from serogroups B to E) among the 294 bacterial strains in a slide agglutination test. No false-positive reactions were detected.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Bacterialen_US
dc.subject.meshAntibodies, Monoclonal - Diagnostic Useen_US
dc.subject.meshAntigens, Bacterial - Chemistryen_US
dc.subject.meshCarbohydrate Sequenceen_US
dc.subject.meshEpitopes - Chemistryen_US
dc.subject.meshHumansen_US
dc.subject.meshLipopolysaccharides - Chemistry - Immunologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshSalmonella - Classification - Immunologyen_US
dc.subject.meshSalmonella Infections - Diagnosisen_US
dc.subject.meshSerotypingen_US
dc.titleAnti-Salmonella lipopolysaccharide monoclonal antibodies: Characterization of Salmonella BO-, CO-, DO-, and EO-specific clones and their diagnostic usefulnessen_US
dc.typeArticleen_US
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_US
dc.identifier.authorityLuk, JMC=rp00349en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1723070-
dc.identifier.scopuseid_2-s2.0-0026000613en_US
dc.identifier.volume29en_US
dc.identifier.issue11en_US
dc.identifier.spage2424en_US
dc.identifier.epage2433en_US
dc.identifier.isiWOS:A1991GK77700012-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLuk, JMC=7006777791en_US
dc.identifier.scopusauthoridLindberg, AA=7101776377en_US

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