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- Publisher Website: 10.1016/0022-1759(90)90445-2
- Scopus: eid_2-s2.0-0025280849
- PMID: 1693643
- WOS: WOS:A1990DG98200012
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Article: Efficient production of mouse and rat monoclonal antibodies against the O antigens of Salmonella serogroup C1, using LPS-coated bacteria as immunogen
Title | Efficient production of mouse and rat monoclonal antibodies against the O antigens of Salmonella serogroup C1, using LPS-coated bacteria as immunogen |
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Authors | |
Keywords | (Mouse) (Rat) Monoclonal antibody O:6,7 lipopolysaccharide Salmonella serogroup C1 |
Issue Date | 1990 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim |
Citation | Journal Of Immunological Methods, 1990, v. 129 n. 2, p. 243-250 How to Cite? |
Abstract | A variety of different immunogens and immunisation schemes were investigated for the production of monoclonal antibodies directed against the O antigenic polysaccharide of the lipopolysaccharide (LPS) of Salmonella serogroup C1 (O: 6,7). Of 12 fusions performed, higher yields of stable, LPS-reactive hybridomas producing monoclonal antibodies were achieved in both the mouse and rat when using O:6,7 LPS-coated S. thompson bacteria as immunogens than with live and heat-killed bacteria, or O:6,7-BSA glycoconjugate as immunogens. All of the 17 hybrid clones obtained were shown to bind the O antigens of Salmonella serogroup C1 when tested in ELISA against a set of chemically defined LPS from Salmonella smooth and rough strains. The results are discussed with a view to bettering the immunisation strategy for production of monoclonal antibodies against the LPS antigens of bacteria. |
Persistent Identifier | http://hdl.handle.net/10722/172630 |
ISSN | 2023 Impact Factor: 1.6 2023 SCImago Journal Rankings: 0.555 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Luk, JMC | en_US |
dc.contributor.author | Nnalue, NA | en_US |
dc.contributor.author | Lindberg, AA | en_US |
dc.date.accessioned | 2012-10-30T06:23:53Z | - |
dc.date.available | 2012-10-30T06:23:53Z | - |
dc.date.issued | 1990 | en_US |
dc.identifier.citation | Journal Of Immunological Methods, 1990, v. 129 n. 2, p. 243-250 | en_US |
dc.identifier.issn | 0022-1759 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/172630 | - |
dc.description.abstract | A variety of different immunogens and immunisation schemes were investigated for the production of monoclonal antibodies directed against the O antigenic polysaccharide of the lipopolysaccharide (LPS) of Salmonella serogroup C1 (O: 6,7). Of 12 fusions performed, higher yields of stable, LPS-reactive hybridomas producing monoclonal antibodies were achieved in both the mouse and rat when using O:6,7 LPS-coated S. thompson bacteria as immunogens than with live and heat-killed bacteria, or O:6,7-BSA glycoconjugate as immunogens. All of the 17 hybrid clones obtained were shown to bind the O antigens of Salmonella serogroup C1 when tested in ELISA against a set of chemically defined LPS from Salmonella smooth and rough strains. The results are discussed with a view to bettering the immunisation strategy for production of monoclonal antibodies against the LPS antigens of bacteria. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim | en_US |
dc.relation.ispartof | Journal of Immunological Methods | en_US |
dc.subject | (Mouse) | - |
dc.subject | (Rat) | - |
dc.subject | Monoclonal antibody | - |
dc.subject | O:6,7 lipopolysaccharide | - |
dc.subject | Salmonella serogroup C1 | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibodies, Monoclonal - Biosynthesis | en_US |
dc.subject.mesh | Antibody Specificity | en_US |
dc.subject.mesh | Antigens, Bacterial - Immunology | en_US |
dc.subject.mesh | Hybridomas - Metabolism | en_US |
dc.subject.mesh | Immunoglobulin Isotypes | en_US |
dc.subject.mesh | Lipopolysaccharides - Immunology | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Mice, Inbred Balb C | en_US |
dc.subject.mesh | O Antigens | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Salmonella - Immunology | en_US |
dc.subject.mesh | Vaccination - Methods | en_US |
dc.title | Efficient production of mouse and rat monoclonal antibodies against the O antigens of Salmonella serogroup C1, using LPS-coated bacteria as immunogen | en_US |
dc.type | Article | en_US |
dc.identifier.email | Luk, JMC: jmluk@hkucc.hku.hk | en_US |
dc.identifier.authority | Luk, JMC=rp00349 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/0022-1759(90)90445-2 | en_US |
dc.identifier.pmid | 1693643 | en_US |
dc.identifier.scopus | eid_2-s2.0-0025280849 | en_US |
dc.identifier.volume | 129 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 243 | en_US |
dc.identifier.epage | 250 | en_US |
dc.identifier.isi | WOS:A1990DG98200012 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Luk, JMC=7006777791 | en_US |
dc.identifier.scopusauthorid | Nnalue, NA=6603867577 | en_US |
dc.identifier.scopusauthorid | Lindberg, AA=7101776377 | en_US |
dc.identifier.issnl | 0022-1759 | - |