File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Efficient production of mouse and rat monoclonal antibodies against the O antigens of Salmonella serogroup C1, using LPS-coated bacteria as immunogen

TitleEfficient production of mouse and rat monoclonal antibodies against the O antigens of Salmonella serogroup C1, using LPS-coated bacteria as immunogen
Authors
Issue Date1990
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim
Citation
Journal Of Immunological Methods, 1990, v. 129 n. 2, p. 243-250 How to Cite?
AbstractA variety of different immunogens and immunisation schemes were investigated for the production of monoclonal antibodies directed against the O antigenic polysaccharide of the lipopolysaccharide (LPS) of Salmonella serogroup C1 (O: 6,7). Of 12 fusions performed, higher yields of stable, LPS-reactive hybridomas producing monoclonal antibodies were achieved in both the mouse and rat when using O:6,7 LPS-coated S. thompson bacteria as immunogens than with live and heat-killed bacteria, or O:6,7-BSA glycoconjugate as immunogens. All of the 17 hybrid clones obtained were shown to bind the O antigens of Salmonella serogroup C1 when tested in ELISA against a set of chemically defined LPS from Salmonella smooth and rough strains. The results are discussed with a view to bettering the immunisation strategy for production of monoclonal antibodies against the LPS antigens of bacteria.
Persistent Identifierhttp://hdl.handle.net/10722/172630
ISSN
2015 Impact Factor: 1.858
2015 SCImago Journal Rankings: 1.053
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLuk, JMCen_US
dc.contributor.authorNnalue, NAen_US
dc.contributor.authorLindberg, AAen_US
dc.date.accessioned2012-10-30T06:23:53Z-
dc.date.available2012-10-30T06:23:53Z-
dc.date.issued1990en_US
dc.identifier.citationJournal Of Immunological Methods, 1990, v. 129 n. 2, p. 243-250en_US
dc.identifier.issn0022-1759en_US
dc.identifier.urihttp://hdl.handle.net/10722/172630-
dc.description.abstractA variety of different immunogens and immunisation schemes were investigated for the production of monoclonal antibodies directed against the O antigenic polysaccharide of the lipopolysaccharide (LPS) of Salmonella serogroup C1 (O: 6,7). Of 12 fusions performed, higher yields of stable, LPS-reactive hybridomas producing monoclonal antibodies were achieved in both the mouse and rat when using O:6,7 LPS-coated S. thompson bacteria as immunogens than with live and heat-killed bacteria, or O:6,7-BSA glycoconjugate as immunogens. All of the 17 hybrid clones obtained were shown to bind the O antigens of Salmonella serogroup C1 when tested in ELISA against a set of chemically defined LPS from Salmonella smooth and rough strains. The results are discussed with a view to bettering the immunisation strategy for production of monoclonal antibodies against the LPS antigens of bacteria.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jimen_US
dc.relation.ispartofJournal of Immunological Methodsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal - Biosynthesisen_US
dc.subject.meshAntibody Specificityen_US
dc.subject.meshAntigens, Bacterial - Immunologyen_US
dc.subject.meshHybridomas - Metabolismen_US
dc.subject.meshImmunoglobulin Isotypesen_US
dc.subject.meshLipopolysaccharides - Immunologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshO Antigensen_US
dc.subject.meshRatsen_US
dc.subject.meshSalmonella - Immunologyen_US
dc.subject.meshVaccination - Methodsen_US
dc.titleEfficient production of mouse and rat monoclonal antibodies against the O antigens of Salmonella serogroup C1, using LPS-coated bacteria as immunogenen_US
dc.typeArticleen_US
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_US
dc.identifier.authorityLuk, JMC=rp00349en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0022-1759(90)90445-2en_US
dc.identifier.pmid1693643en_US
dc.identifier.scopuseid_2-s2.0-0025280849en_US
dc.identifier.volume129en_US
dc.identifier.issue2en_US
dc.identifier.spage243en_US
dc.identifier.epage250en_US
dc.identifier.isiWOS:A1990DG98200012-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLuk, JMC=7006777791en_US
dc.identifier.scopusauthoridNnalue, NA=6603867577en_US
dc.identifier.scopusauthoridLindberg, AA=7101776377en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats