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Article: Detection in rabbit sera of blocking antibodies against staphylococcal fibronectin-binding protein by enzyme-linked immunosorbent assay
Title | Detection in rabbit sera of blocking antibodies against staphylococcal fibronectin-binding protein by enzyme-linked immunosorbent assay |
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Authors | |
Keywords | Antibody ELISA Fibronectin binding protein Staphylococcus aureus |
Issue Date | 1989 |
Citation | Fems Microbiology Immunology, 1989, v. 47 n. 8-9, p. 505-510 How to Cite? |
Abstract | Antibody titres against fibronectin-binding protein (FnBP) of Staphylococcus aureus were determined in sera from rabbits immunized with staphylococcal whole cells or purified native fibronectin receptor. An ELISA technique for detection of antibody titres blocking the binding of soluble fibronectin to immobilized FnBP was developed. A recombinant staphylococcal FnBP fused to E. coli β-galactosidase (gal-FnBp) was used as the immobilized antigen in this test. Serum samples from two different rabbits immunized with native fibronectin receptor gave significant blocking titres, whereas the blocking titres of antisera against staphylococcal whole cells were about 4- to 5-fold lower. Using the gal-FnBP fusion protein, the sensitivity for detection of fibronectin by ELISA was also determined. The detection limit is around 5 ng. The findings are discussed with a view to developing an anti-staphylococcal adherence vaccine and quantitating fibronectin in solution. |
Persistent Identifier | http://hdl.handle.net/10722/172613 |
ISSN |
DC Field | Value | Language |
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dc.contributor.author | Luk, JMC | en_US |
dc.contributor.author | Flock, JI | en_US |
dc.contributor.author | Wadstrom, T | en_US |
dc.date.accessioned | 2012-10-30T06:23:44Z | - |
dc.date.available | 2012-10-30T06:23:44Z | - |
dc.date.issued | 1989 | en_US |
dc.identifier.citation | Fems Microbiology Immunology, 1989, v. 47 n. 8-9, p. 505-510 | en_US |
dc.identifier.issn | 0920-8534 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/172613 | - |
dc.description.abstract | Antibody titres against fibronectin-binding protein (FnBP) of Staphylococcus aureus were determined in sera from rabbits immunized with staphylococcal whole cells or purified native fibronectin receptor. An ELISA technique for detection of antibody titres blocking the binding of soluble fibronectin to immobilized FnBP was developed. A recombinant staphylococcal FnBP fused to E. coli β-galactosidase (gal-FnBp) was used as the immobilized antigen in this test. Serum samples from two different rabbits immunized with native fibronectin receptor gave significant blocking titres, whereas the blocking titres of antisera against staphylococcal whole cells were about 4- to 5-fold lower. Using the gal-FnBP fusion protein, the sensitivity for detection of fibronectin by ELISA was also determined. The detection limit is around 5 ng. The findings are discussed with a view to developing an anti-staphylococcal adherence vaccine and quantitating fibronectin in solution. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | FEMS Microbiology Immunology | en_US |
dc.subject | Antibody | - |
dc.subject | ELISA | - |
dc.subject | Fibronectin binding protein | - |
dc.subject | Staphylococcus aureus | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibodies, Bacterial - Analysis | en_US |
dc.subject.mesh | Binding, Competitive | en_US |
dc.subject.mesh | Enzyme-Linked Immunosorbent Assay | en_US |
dc.subject.mesh | Fibronectins - Metabolism | en_US |
dc.subject.mesh | Rabbits | en_US |
dc.subject.mesh | Receptors, Fibronectin | en_US |
dc.subject.mesh | Receptors, Immunologic - Immunology | en_US |
dc.subject.mesh | Recombinant Fusion Proteins - Immunology | en_US |
dc.subject.mesh | Staphylococcus Aureus - Immunology | en_US |
dc.subject.mesh | Beta-Galactosidase - Immunology | en_US |
dc.title | Detection in rabbit sera of blocking antibodies against staphylococcal fibronectin-binding protein by enzyme-linked immunosorbent assay | en_US |
dc.type | Article | en_US |
dc.identifier.email | Luk, JMC: jmluk@hkucc.hku.hk | en_US |
dc.identifier.authority | Luk, JMC=rp00349 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 2534053 | - |
dc.identifier.scopus | eid_2-s2.0-0024796439 | en_US |
dc.identifier.volume | 47 | en_US |
dc.identifier.issue | 8-9 | en_US |
dc.identifier.spage | 505 | en_US |
dc.identifier.epage | 510 | en_US |
dc.identifier.scopusauthorid | Luk, JMC=7006777791 | en_US |
dc.identifier.scopusauthorid | Flock, JI=7005627375 | en_US |
dc.identifier.scopusauthorid | Wadstrom, T=36050694600 | en_US |
dc.identifier.issnl | 0920-8534 | - |