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Article: Detection in rabbit sera of blocking antibodies against staphylococcal fibronectin-binding protein by enzyme-linked immunosorbent assay

TitleDetection in rabbit sera of blocking antibodies against staphylococcal fibronectin-binding protein by enzyme-linked immunosorbent assay
Authors
Issue Date1989
Citation
Fems Microbiology Immunology, 1989, v. 47 n. 8-9, p. 505-510 How to Cite?
AbstractAntibody titres against fibronectin-binding protein (FnBP) of Staphylococcus aureus were determined in sera from rabbits immunized with staphylococcal whole cells or purified native fibronectin receptor. An ELISA technique for detection of antibody titres blocking the binding of soluble fibronectin to immobilized FnBP was developed. A recombinant staphylococcal FnBP fused to E. coli β-galactosidase (gal-FnBp) was used as the immobilized antigen in this test. Serum samples from two different rabbits immunized with native fibronectin receptor gave significant blocking titres, whereas the blocking titres of antisera against staphylococcal whole cells were about 4- to 5-fold lower. Using the gal-FnBP fusion protein, the sensitivity for detection of fibronectin by ELISA was also determined. The detection limit is around 5 ng. The findings are discussed with a view to developing an anti-staphylococcal adherence vaccine and quantitating fibronectin in solution.
Persistent Identifierhttp://hdl.handle.net/10722/172613
ISSN

 

DC FieldValueLanguage
dc.contributor.authorLuk, JMCen_US
dc.contributor.authorFlock, JIen_US
dc.contributor.authorWadstrom, Ten_US
dc.date.accessioned2012-10-30T06:23:44Z-
dc.date.available2012-10-30T06:23:44Z-
dc.date.issued1989en_US
dc.identifier.citationFems Microbiology Immunology, 1989, v. 47 n. 8-9, p. 505-510en_US
dc.identifier.issn0920-8534en_US
dc.identifier.urihttp://hdl.handle.net/10722/172613-
dc.description.abstractAntibody titres against fibronectin-binding protein (FnBP) of Staphylococcus aureus were determined in sera from rabbits immunized with staphylococcal whole cells or purified native fibronectin receptor. An ELISA technique for detection of antibody titres blocking the binding of soluble fibronectin to immobilized FnBP was developed. A recombinant staphylococcal FnBP fused to E. coli β-galactosidase (gal-FnBp) was used as the immobilized antigen in this test. Serum samples from two different rabbits immunized with native fibronectin receptor gave significant blocking titres, whereas the blocking titres of antisera against staphylococcal whole cells were about 4- to 5-fold lower. Using the gal-FnBP fusion protein, the sensitivity for detection of fibronectin by ELISA was also determined. The detection limit is around 5 ng. The findings are discussed with a view to developing an anti-staphylococcal adherence vaccine and quantitating fibronectin in solution.en_US
dc.languageengen_US
dc.relation.ispartofFEMS Microbiology Immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Bacterial - Analysisen_US
dc.subject.meshBinding, Competitiveen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFibronectins - Metabolismen_US
dc.subject.meshRabbitsen_US
dc.subject.meshReceptors, Fibronectinen_US
dc.subject.meshReceptors, Immunologic - Immunologyen_US
dc.subject.meshRecombinant Fusion Proteins - Immunologyen_US
dc.subject.meshStaphylococcus Aureus - Immunologyen_US
dc.subject.meshBeta-Galactosidase - Immunologyen_US
dc.titleDetection in rabbit sera of blocking antibodies against staphylococcal fibronectin-binding protein by enzyme-linked immunosorbent assayen_US
dc.typeArticleen_US
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_US
dc.identifier.authorityLuk, JMC=rp00349en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2534053-
dc.identifier.scopuseid_2-s2.0-0024796439en_US
dc.identifier.volume47en_US
dc.identifier.issue8-9en_US
dc.identifier.spage505en_US
dc.identifier.epage510en_US
dc.identifier.scopusauthoridLuk, JMC=7006777791en_US
dc.identifier.scopusauthoridFlock, JI=7005627375en_US
dc.identifier.scopusauthoridWadstrom, T=36050694600en_US

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