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Article: Characterisation and application of a murine monoclonal antibody specific for the serogroup C2 Salmonella

TitleCharacterisation and application of a murine monoclonal antibody specific for the serogroup C2 Salmonella
Authors
Issue Date1988
PublisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
Citation
Journal Of Medical Microbiology, 1988, v. 26 n. 2, p. 115-119 How to Cite?
AbstractAn IgG3 murine monoclonal antibody (designated MO8) specific for the serogroup C2 Salmonella lipopolysaccharide (LPS) was generated by fusing mouse myeloma cells NS1 with spleen cells of BALB/c mice immunised with heat-killed S. manhattan. MO8 reacted with purified LPS prepared from serogroup C2 Salmonella but did not react with that prepared from other O serogroups, and its reactivity was also specifically absorbed by serogroup C2 Salmonella only. Polyacrylamide gel electrophoresis of the serogroup C2 LPS and subsequent immunoblotting with MO8 yielded multiple reactive bands giving a characteristic ladder pattern. The specificity of MO8 was further demonstrated in the slide agglutination test with 223 bacteria, of which only 25 belonging to serogroup C2 Salmonella reacted with the MO8 ascitic fluid. The specificity of MO8 makes it useful not only for the serological identification of Salmonella but also for the epitope analysis of the serogroup C2 LPS.
Persistent Identifierhttp://hdl.handle.net/10722/172585
ISSN
2015 Impact Factor: 2.269
2015 SCImago Journal Rankings: 1.060
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLuk, JMCen_US
dc.contributor.authorChan, KHen_US
dc.contributor.authorTsang, RSWen_US
dc.contributor.authorNg, MHen_US
dc.date.accessioned2012-10-30T06:23:33Z-
dc.date.available2012-10-30T06:23:33Z-
dc.date.issued1988en_US
dc.identifier.citationJournal Of Medical Microbiology, 1988, v. 26 n. 2, p. 115-119en_US
dc.identifier.issn0022-2615en_US
dc.identifier.urihttp://hdl.handle.net/10722/172585-
dc.description.abstractAn IgG3 murine monoclonal antibody (designated MO8) specific for the serogroup C2 Salmonella lipopolysaccharide (LPS) was generated by fusing mouse myeloma cells NS1 with spleen cells of BALB/c mice immunised with heat-killed S. manhattan. MO8 reacted with purified LPS prepared from serogroup C2 Salmonella but did not react with that prepared from other O serogroups, and its reactivity was also specifically absorbed by serogroup C2 Salmonella only. Polyacrylamide gel electrophoresis of the serogroup C2 LPS and subsequent immunoblotting with MO8 yielded multiple reactive bands giving a characteristic ladder pattern. The specificity of MO8 was further demonstrated in the slide agglutination test with 223 bacteria, of which only 25 belonging to serogroup C2 Salmonella reacted with the MO8 ascitic fluid. The specificity of MO8 makes it useful not only for the serological identification of Salmonella but also for the epitope analysis of the serogroup C2 LPS.en_US
dc.languageengen_US
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.orgen_US
dc.relation.ispartofJournal of Medical Microbiologyen_US
dc.subject.meshAgglutination Testsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal - Biosynthesis - Immunologyen_US
dc.subject.meshAntibody Specificityen_US
dc.subject.meshAntigens, Surface - Immunologyen_US
dc.subject.meshBinding, Competitiveen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshEpitopes - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshHybridomasen_US
dc.subject.meshImmunoassayen_US
dc.subject.meshImmunoglobulin G - Immunologyen_US
dc.subject.meshLipopolysaccharides - Immunologyen_US
dc.subject.meshMiceen_US
dc.subject.meshSalmonella - Immunologyen_US
dc.titleCharacterisation and application of a murine monoclonal antibody specific for the serogroup C2 Salmonellaen_US
dc.typeArticleen_US
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_US
dc.identifier.authorityLuk, JMC=rp00349en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1099/00222615-26-2-115-
dc.identifier.pmid2455055-
dc.identifier.scopuseid_2-s2.0-0023909159en_US
dc.identifier.volume26en_US
dc.identifier.issue2en_US
dc.identifier.spage115en_US
dc.identifier.epage119en_US
dc.identifier.isiWOS:A1988N885700006-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLuk, JMC=7006777791en_US
dc.identifier.scopusauthoridChan, KH=35338760600en_US
dc.identifier.scopusauthoridTsang, RSW=7102940066en_US
dc.identifier.scopusauthoridNg, MH=7202076421en_US

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