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Article: A novel fluorescent cell membrane-permeable caged cyclic ADP-ribose analogue

TitleA novel fluorescent cell membrane-permeable caged cyclic ADP-ribose analogue
Authors
Issue Date2012
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal of Biological Chemistry, 2012, v. 287 n. 29, p. 24774-24783 How to Cite?
AbstractCyclic adenosine diphosphate ribose is an endogenous Ca(2+) mobilizer involved in diverse cellular processes. A cell membrane-permeable cyclic adenosine diphosphate ribose analogue, cyclic inosine diphosphoribose ether (cIDPRE), can induce Ca(2+) increase in intact human Jurkat T-lymphocytes. Here we synthesized a coumarin-caged analogue of cIDPRE (Co-i-cIDPRE), aiming to have a precisely temporal and spatial control of bioactive cIDPRE release inside the cell using UV uncaging. We showed that Co-i-cIDPRE accumulated inside Jurkat cells quickly and efficiently. Uncaging of Co-i-cIDPRE evoked Ca(2+) release from endoplasmic reticulum, with concomitant Ca(2+) influx in Jurkat cells. Ca(2+) release evoked by uncaged Co-i-cIDPRE was blocked by knockdown of ryanodine receptors (RyRs) 2 and 3 in Jurkat cells. The associated Ca(2+) influx, on the other hand, was abolished by double knockdown of Stim1 and TRPM2 in Jurkat cells. Furthermore, Ca(2+) release or influx evoked by uncaged Co-i-cIDPRE was recapitulated in HEK293 cells that overexpress RyRs or TRPM2, respectively, but not in wild-type cells lacking these channels. In summary, our results indicate that uncaging of Co-i-cIDPRE incites Ca(2+) release from endoplasmic reticulum via RyRs and triggers Ca(2+) influx via TRPM2.
Persistent Identifierhttp://hdl.handle.net/10722/171794
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYu, PLen_US
dc.contributor.authorZhang, ZHen_US
dc.contributor.authorHao, BXen_US
dc.contributor.authorZhao, YJen_US
dc.contributor.authorZhang, LHen_US
dc.contributor.authorLee, HCen_US
dc.contributor.authorZhang, Len_US
dc.contributor.authorYue, Jen_US
dc.date.accessioned2012-10-30T06:17:09Z-
dc.date.available2012-10-30T06:17:09Z-
dc.date.issued2012en_US
dc.identifier.citationJournal of Biological Chemistry, 2012, v. 287 n. 29, p. 24774-24783en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/171794-
dc.description.abstractCyclic adenosine diphosphate ribose is an endogenous Ca(2+) mobilizer involved in diverse cellular processes. A cell membrane-permeable cyclic adenosine diphosphate ribose analogue, cyclic inosine diphosphoribose ether (cIDPRE), can induce Ca(2+) increase in intact human Jurkat T-lymphocytes. Here we synthesized a coumarin-caged analogue of cIDPRE (Co-i-cIDPRE), aiming to have a precisely temporal and spatial control of bioactive cIDPRE release inside the cell using UV uncaging. We showed that Co-i-cIDPRE accumulated inside Jurkat cells quickly and efficiently. Uncaging of Co-i-cIDPRE evoked Ca(2+) release from endoplasmic reticulum, with concomitant Ca(2+) influx in Jurkat cells. Ca(2+) release evoked by uncaged Co-i-cIDPRE was blocked by knockdown of ryanodine receptors (RyRs) 2 and 3 in Jurkat cells. The associated Ca(2+) influx, on the other hand, was abolished by double knockdown of Stim1 and TRPM2 in Jurkat cells. Furthermore, Ca(2+) release or influx evoked by uncaged Co-i-cIDPRE was recapitulated in HEK293 cells that overexpress RyRs or TRPM2, respectively, but not in wild-type cells lacking these channels. In summary, our results indicate that uncaging of Co-i-cIDPRE incites Ca(2+) release from endoplasmic reticulum via RyRs and triggers Ca(2+) influx via TRPM2.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.-
dc.rightsThis research was originally published in [Journal of Biological Chemistry]. Yu, PL;Zhang, ZH;Hao, BX;Zhao, YJ;Zhang, LH;Lee, HC;Zhang, L;Yue, J. A novel fluorescent cell membrane-permeable caged cyclic ADP-ribose analogue. Journal of Biological Chemistry. 2012. Vol:pp24774-pp.24783 © the American Society for Biochemistry and Molecular Biology-
dc.subject.meshAlkenes - metabolism-
dc.subject.meshCalcium-
dc.subject.meshCell Membrane - metabolism-
dc.subject.meshCoumarins - metabolism-
dc.subject.meshCyclic ADP-Ribose - analogs and derivatives - metabolism-
dc.titleA novel fluorescent cell membrane-permeable caged cyclic ADP-ribose analogueen_US
dc.typeArticleen_US
dc.identifier.emailYu, PL: ypljj@hku.hken_US
dc.identifier.emailZhao, YJ: yongjuan@hkucc.hku.hken_US
dc.identifier.emailLee, HC: leehc@hku.hk-
dc.identifier.emailZhang, L: liangren@bjmu.edu.cn-
dc.identifier.emailYue, J: jyue@hku.hk-
dc.identifier.authorityLee, HC=rp00545en_US
dc.identifier.authorityYue, J=rp00286en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1074/jbc.M111.329854en_US
dc.identifier.pmid22661714-
dc.identifier.pmcidPMC3397904-
dc.identifier.scopuseid_2-s2.0-84863798406en_US
dc.identifier.hkuros202948-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84863798406&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume287en_US
dc.identifier.issue29en_US
dc.identifier.spage24774en_US
dc.identifier.epage24783en_US
dc.identifier.isiWOS:000306651100070-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYue, J=7101875828en_US
dc.identifier.scopusauthoridZhang, L=8833065300en_US
dc.identifier.scopusauthoridLee, HC=26642959100en_US
dc.identifier.scopusauthoridZhang, LH=10340097000en_US
dc.identifier.scopusauthoridZhao, YJ=35219477000en_US
dc.identifier.scopusauthoridHao, BX=55276400800en_US
dc.identifier.scopusauthoridZhang, ZH=55312678000en_US
dc.identifier.scopusauthoridYu, PL=55034912300en_US

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