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Article: Mos mediates the mitotic activation of p42 MAPK in Xenopus egg extracts

TitleMos mediates the mitotic activation of p42 MAPK in Xenopus egg extracts
Authors
Issue Date2004
PublisherCell Press. The Journal's web site is located at http://www.current-biology.com/
Citation
Current Biology, 2004, v. 14 n. 17, p. 1581-1586 How to Cite?
AbstractThe ERK1/ERK2 MAP kinases (MAPKs) are transiently activated during mitosis, and MAPK activation has been implicated in the spindle assembly checkpoint and in establishing the timing of an unperturbed mitosis [1-4]. The MAPK activator MEK1 is required for mitotic activation of p42 MAPK in Xenopus egg extracts; however, the identity of the kinase that activates MEK1 is unknown. Here we have partially purified a Cdc2-cyclin B-induced MEK-activating protein kinase from mitotic Xenopus egg extracts and identified it as the Mos protooncoprotein, a MAP kinase kinase kinase present at low levels in mitotic egg extracts, early embryos, and somatic cells. Immunodepletion of Mos from interphase egg extracts was found to abolish Δ90 cyclin B-Cdc2-stimulated p42 MAPK activation. In contrast, immunodepletion of Raf-1 and B-Raf, two other MEK-activating kinases present in Xenopus egg extracts, had little effect on cyclin-stimulated p42 MAPK activation. Immunodepletion of Mos also abolished the transient activation of p42 MAPK in cycling egg extracts. Taken together, these data demonstrate that Mos is responsible for the mitotic activation of the p42 MAPK pathway in Xenopus egg extracts.
Persistent Identifierhttp://hdl.handle.net/10722/171767
ISSN
2015 Impact Factor: 8.983
2015 SCImago Journal Rankings: 4.729
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYue, Jen_US
dc.contributor.authorFerrell Jr, JEen_US
dc.date.accessioned2012-10-30T06:16:55Z-
dc.date.available2012-10-30T06:16:55Z-
dc.date.issued2004en_US
dc.identifier.citationCurrent Biology, 2004, v. 14 n. 17, p. 1581-1586en_US
dc.identifier.issn0960-9822en_US
dc.identifier.urihttp://hdl.handle.net/10722/171767-
dc.description.abstractThe ERK1/ERK2 MAP kinases (MAPKs) are transiently activated during mitosis, and MAPK activation has been implicated in the spindle assembly checkpoint and in establishing the timing of an unperturbed mitosis [1-4]. The MAPK activator MEK1 is required for mitotic activation of p42 MAPK in Xenopus egg extracts; however, the identity of the kinase that activates MEK1 is unknown. Here we have partially purified a Cdc2-cyclin B-induced MEK-activating protein kinase from mitotic Xenopus egg extracts and identified it as the Mos protooncoprotein, a MAP kinase kinase kinase present at low levels in mitotic egg extracts, early embryos, and somatic cells. Immunodepletion of Mos from interphase egg extracts was found to abolish Δ90 cyclin B-Cdc2-stimulated p42 MAPK activation. In contrast, immunodepletion of Raf-1 and B-Raf, two other MEK-activating kinases present in Xenopus egg extracts, had little effect on cyclin-stimulated p42 MAPK activation. Immunodepletion of Mos also abolished the transient activation of p42 MAPK in cycling egg extracts. Taken together, these data demonstrate that Mos is responsible for the mitotic activation of the p42 MAPK pathway in Xenopus egg extracts.en_US
dc.languageengen_US
dc.publisherCell Press. The Journal's web site is located at http://www.current-biology.com/en_US
dc.relation.ispartofCurrent Biologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAutoradiographyen_US
dc.subject.meshCell Extractsen_US
dc.subject.meshChromatography, Agaroseen_US
dc.subject.meshChromatography, Gelen_US
dc.subject.meshCyclin B - Metabolismen_US
dc.subject.meshEnzyme Activation - Physiologyen_US
dc.subject.meshImmunoprecipitationen_US
dc.subject.meshMitogen-Activated Protein Kinase 1 - Metabolismen_US
dc.subject.meshMitogen-Activated Protein Kinase Kinases - Metabolismen_US
dc.subject.meshMitosis - Physiologyen_US
dc.subject.meshOvum - Cytology - Metabolismen_US
dc.subject.meshProto-Oncogene Proteins C-Mos - Genetics - Metabolismen_US
dc.subject.meshSignal Transduction - Physiologyen_US
dc.subject.meshXenopus - Metabolismen_US
dc.subject.meshXenopus Proteins - Genetics - Metabolismen_US
dc.titleMos mediates the mitotic activation of p42 MAPK in Xenopus egg extractsen_US
dc.typeArticleen_US
dc.identifier.emailYue, J:jyue@hku.hken_US
dc.identifier.authorityYue, J=rp00286en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.cub.2004.08.056en_US
dc.identifier.pmid15341746-
dc.identifier.scopuseid_2-s2.0-4644345203en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-4644345203&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume14en_US
dc.identifier.issue17en_US
dc.identifier.spage1581en_US
dc.identifier.epage1586en_US
dc.identifier.isiWOS:000223813500025-
dc.publisher.placeUnited Statesen_US
dc.identifier.f10001021158-
dc.identifier.scopusauthoridYue, J=7101875828en_US
dc.identifier.scopusauthoridFerrell Jr, JE=7005371587en_US

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