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- Publisher Website: 10.1016/j.cub.2004.08.056
- Scopus: eid_2-s2.0-4644345203
- PMID: 15341746
- WOS: WOS:000223813500025
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Article: Mos mediates the mitotic activation of p42 MAPK in Xenopus egg extracts
Title | Mos mediates the mitotic activation of p42 MAPK in Xenopus egg extracts |
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Authors | |
Issue Date | 2004 |
Publisher | Cell Press. The Journal's web site is located at http://www.current-biology.com/ |
Citation | Current Biology, 2004, v. 14 n. 17, p. 1581-1586 How to Cite? |
Abstract | The ERK1/ERK2 MAP kinases (MAPKs) are transiently activated during mitosis, and MAPK activation has been implicated in the spindle assembly checkpoint and in establishing the timing of an unperturbed mitosis [1-4]. The MAPK activator MEK1 is required for mitotic activation of p42 MAPK in Xenopus egg extracts; however, the identity of the kinase that activates MEK1 is unknown. Here we have partially purified a Cdc2-cyclin B-induced MEK-activating protein kinase from mitotic Xenopus egg extracts and identified it as the Mos protooncoprotein, a MAP kinase kinase kinase present at low levels in mitotic egg extracts, early embryos, and somatic cells. Immunodepletion of Mos from interphase egg extracts was found to abolish Δ90 cyclin B-Cdc2-stimulated p42 MAPK activation. In contrast, immunodepletion of Raf-1 and B-Raf, two other MEK-activating kinases present in Xenopus egg extracts, had little effect on cyclin-stimulated p42 MAPK activation. Immunodepletion of Mos also abolished the transient activation of p42 MAPK in cycling egg extracts. Taken together, these data demonstrate that Mos is responsible for the mitotic activation of the p42 MAPK pathway in Xenopus egg extracts. |
Persistent Identifier | http://hdl.handle.net/10722/171767 |
ISSN | 2023 Impact Factor: 8.1 2023 SCImago Journal Rankings: 2.982 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Yue, J | en_US |
dc.contributor.author | Ferrell Jr, JE | en_US |
dc.date.accessioned | 2012-10-30T06:16:55Z | - |
dc.date.available | 2012-10-30T06:16:55Z | - |
dc.date.issued | 2004 | en_US |
dc.identifier.citation | Current Biology, 2004, v. 14 n. 17, p. 1581-1586 | en_US |
dc.identifier.issn | 0960-9822 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171767 | - |
dc.description.abstract | The ERK1/ERK2 MAP kinases (MAPKs) are transiently activated during mitosis, and MAPK activation has been implicated in the spindle assembly checkpoint and in establishing the timing of an unperturbed mitosis [1-4]. The MAPK activator MEK1 is required for mitotic activation of p42 MAPK in Xenopus egg extracts; however, the identity of the kinase that activates MEK1 is unknown. Here we have partially purified a Cdc2-cyclin B-induced MEK-activating protein kinase from mitotic Xenopus egg extracts and identified it as the Mos protooncoprotein, a MAP kinase kinase kinase present at low levels in mitotic egg extracts, early embryos, and somatic cells. Immunodepletion of Mos from interphase egg extracts was found to abolish Δ90 cyclin B-Cdc2-stimulated p42 MAPK activation. In contrast, immunodepletion of Raf-1 and B-Raf, two other MEK-activating kinases present in Xenopus egg extracts, had little effect on cyclin-stimulated p42 MAPK activation. Immunodepletion of Mos also abolished the transient activation of p42 MAPK in cycling egg extracts. Taken together, these data demonstrate that Mos is responsible for the mitotic activation of the p42 MAPK pathway in Xenopus egg extracts. | en_US |
dc.language | eng | en_US |
dc.publisher | Cell Press. The Journal's web site is located at http://www.current-biology.com/ | en_US |
dc.relation.ispartof | Current Biology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Autoradiography | en_US |
dc.subject.mesh | Cell Extracts | en_US |
dc.subject.mesh | Chromatography, Agarose | en_US |
dc.subject.mesh | Chromatography, Gel | en_US |
dc.subject.mesh | Cyclin B - Metabolism | en_US |
dc.subject.mesh | Enzyme Activation - Physiology | en_US |
dc.subject.mesh | Immunoprecipitation | en_US |
dc.subject.mesh | Mitogen-Activated Protein Kinase 1 - Metabolism | en_US |
dc.subject.mesh | Mitogen-Activated Protein Kinase Kinases - Metabolism | en_US |
dc.subject.mesh | Mitosis - Physiology | en_US |
dc.subject.mesh | Ovum - Cytology - Metabolism | en_US |
dc.subject.mesh | Proto-Oncogene Proteins C-Mos - Genetics - Metabolism | en_US |
dc.subject.mesh | Signal Transduction - Physiology | en_US |
dc.subject.mesh | Xenopus - Metabolism | en_US |
dc.subject.mesh | Xenopus Proteins - Genetics - Metabolism | en_US |
dc.title | Mos mediates the mitotic activation of p42 MAPK in Xenopus egg extracts | en_US |
dc.type | Article | en_US |
dc.identifier.email | Yue, J:jyue@hku.hk | en_US |
dc.identifier.authority | Yue, J=rp00286 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.cub.2004.08.056 | en_US |
dc.identifier.pmid | 15341746 | - |
dc.identifier.scopus | eid_2-s2.0-4644345203 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-4644345203&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 14 | en_US |
dc.identifier.issue | 17 | en_US |
dc.identifier.spage | 1581 | en_US |
dc.identifier.epage | 1586 | en_US |
dc.identifier.isi | WOS:000223813500025 | - |
dc.publisher.place | United States | en_US |
dc.identifier.f1000 | 1021158 | - |
dc.identifier.scopusauthorid | Yue, J=7101875828 | en_US |
dc.identifier.scopusauthorid | Ferrell Jr, JE=7005371587 | en_US |
dc.identifier.issnl | 0960-9822 | - |