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Article: Analysis of submicromolar concentrations of adenosine in plasma using reversed phase high-performance liquid chromatography

TitleAnalysis of submicromolar concentrations of adenosine in plasma using reversed phase high-performance liquid chromatography
Authors
KeywordsAdenosine
Reversed-Phase High-Performance Liquid Chromatography.
Vasodilators
Issue Date1986
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jpba
Citation
Journal Of Pharmaceutical And Biomedical Analysis, 1986, v. 4 n. 2, p. 207-219 How to Cite?
AbstractA method is described for the determination of adenosine in small samples of plasma (< 1 ml) using reversed-phase high-performance liquid chromatography (HPLC) in either a simple isocratic or a gradient elution system which gives a clear separation of adenosine from other plasma constituents. Acetone is used to deproteinize plasma and chloroform to remove unwanted lipid soluble material prior to HPLC. 6-Methyladenosine is used as an internal standard for making corrections for changes in concentration during sample processing. Adenosine in plasma could be reliably detected at concentrations lower than its minimum effector concentration as a vasodilator (4 × 10-8 Mol l-1 using the isocratic system and 1.9 × 10-8 Mol l-1 with gradient elution). The recoveries of adenosine added to blood at concentrations ranging from 2 × 10-8 Mol l-1 to 1.4 × 10-6 Mol l-1 were from 101.4 ± 16.9% (n = 4) to 100.0 ± 3.6% (n = 5). The present method provides a simple, sensitive and selective assay for submicromolar concentrations of adenosine in plasma with good recovery. © 1986.
Persistent Identifierhttp://hdl.handle.net/10722/171761
ISSN
2015 Impact Factor: 3.169
2015 SCImago Journal Rankings: 1.049

 

DC FieldValueLanguage
dc.contributor.authorBallard, HJen_US
dc.contributor.authorCotterrell, Den_US
dc.contributor.authorKarim, Fen_US
dc.date.accessioned2012-10-30T06:16:50Z-
dc.date.available2012-10-30T06:16:50Z-
dc.date.issued1986en_US
dc.identifier.citationJournal Of Pharmaceutical And Biomedical Analysis, 1986, v. 4 n. 2, p. 207-219en_US
dc.identifier.issn0731-7085en_US
dc.identifier.urihttp://hdl.handle.net/10722/171761-
dc.description.abstractA method is described for the determination of adenosine in small samples of plasma (< 1 ml) using reversed-phase high-performance liquid chromatography (HPLC) in either a simple isocratic or a gradient elution system which gives a clear separation of adenosine from other plasma constituents. Acetone is used to deproteinize plasma and chloroform to remove unwanted lipid soluble material prior to HPLC. 6-Methyladenosine is used as an internal standard for making corrections for changes in concentration during sample processing. Adenosine in plasma could be reliably detected at concentrations lower than its minimum effector concentration as a vasodilator (4 × 10-8 Mol l-1 using the isocratic system and 1.9 × 10-8 Mol l-1 with gradient elution). The recoveries of adenosine added to blood at concentrations ranging from 2 × 10-8 Mol l-1 to 1.4 × 10-6 Mol l-1 were from 101.4 ± 16.9% (n = 4) to 100.0 ± 3.6% (n = 5). The present method provides a simple, sensitive and selective assay for submicromolar concentrations of adenosine in plasma with good recovery. © 1986.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jpbaen_US
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysisen_US
dc.subjectAdenosineen_US
dc.subjectReversed-Phase High-Performance Liquid Chromatography.en_US
dc.subjectVasodilatorsen_US
dc.titleAnalysis of submicromolar concentrations of adenosine in plasma using reversed phase high-performance liquid chromatographyen_US
dc.typeArticleen_US
dc.identifier.emailBallard, HJ:ballard@hkucc.hku.hken_US
dc.identifier.authorityBallard, HJ=rp00367en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-38249043986en_US
dc.identifier.volume4en_US
dc.identifier.issue2en_US
dc.identifier.spage207en_US
dc.identifier.epage219en_US
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridBallard, HJ=7005286310en_US
dc.identifier.scopusauthoridCotterrell, D=6602879005en_US
dc.identifier.scopusauthoridKarim, F=7005896790en_US

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