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Article: Adrenomedullin and its receptor components in adipose tissues: Differences between white and brown fats and the effects of adrenergic stimulation

TitleAdrenomedullin and its receptor components in adipose tissues: Differences between white and brown fats and the effects of adrenergic stimulation
Authors
Issue Date2007
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/peptides
Citation
Peptides, 2007, v. 28 n. 4, p. 920-927 How to Cite?
AbstractMale Sprague-Dawley rats were subcutaneously injected with 2.5 mg/kg phenylephrine or 2.5 mg/kg isoproterenol or both (2.5 mg/kg for each drug) for 4 days, twice a day. Samples of scapular brown adipose tissue (BAT) and epididymal white adipose tissue (WAT) were collected for the measurement of adrenomedullin (AM) levels and the gene expression of preproAM, calcitonin receptor like receptor (CRLR) and its activity modifying proteins (RAMPs) by radioimmunoassay and RT-PCR. These values were compared with those in the rats that received 0.9% saline. The gene expression of AM and AM receptor components in BAT are much less than that in epididymal WAT. In BAT there were an increase in AM peptide level after a combined treatment of α1 and β adrenoceptor agonists and increases in preproAM mRNA levels for rats treated with α1 and β receptor agonists alone or in combination. Both CRLR and RAMP2 mRNA levels of αβ group were increased significantly. In WAT, AM peptide level, RAMP1 and RAMP2 mRNA expression levels were augmented in the α group while CRLR mRNA level was enhanced in the β group. The levels of AM, its receptor and RAMPs are much less in BAT than in WAT but adrenergic stimulation has a greater effect on the AM and its receptor components in BAT than those in WAT. AM stimulates lipolysis and increases the level of uncoupling protein-1 (UCP-1) in BAT. It may therefore enhance thermogenesis by increasing the availability of free fatty acids substrate as well as the UCP-1 level on the mitochondrial membrane. © 2006 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/171754
ISSN
2015 Impact Factor: 2.535
2015 SCImago Journal Rankings: 1.128
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGo, AGGen_US
dc.contributor.authorChow, KHMen_US
dc.contributor.authorHwang, ISSen_US
dc.contributor.authorTang, Fen_US
dc.date.accessioned2012-10-30T06:16:48Z-
dc.date.available2012-10-30T06:16:48Z-
dc.date.issued2007en_US
dc.identifier.citationPeptides, 2007, v. 28 n. 4, p. 920-927en_US
dc.identifier.issn0196-9781en_US
dc.identifier.urihttp://hdl.handle.net/10722/171754-
dc.description.abstractMale Sprague-Dawley rats were subcutaneously injected with 2.5 mg/kg phenylephrine or 2.5 mg/kg isoproterenol or both (2.5 mg/kg for each drug) for 4 days, twice a day. Samples of scapular brown adipose tissue (BAT) and epididymal white adipose tissue (WAT) were collected for the measurement of adrenomedullin (AM) levels and the gene expression of preproAM, calcitonin receptor like receptor (CRLR) and its activity modifying proteins (RAMPs) by radioimmunoassay and RT-PCR. These values were compared with those in the rats that received 0.9% saline. The gene expression of AM and AM receptor components in BAT are much less than that in epididymal WAT. In BAT there were an increase in AM peptide level after a combined treatment of α1 and β adrenoceptor agonists and increases in preproAM mRNA levels for rats treated with α1 and β receptor agonists alone or in combination. Both CRLR and RAMP2 mRNA levels of αβ group were increased significantly. In WAT, AM peptide level, RAMP1 and RAMP2 mRNA expression levels were augmented in the α group while CRLR mRNA level was enhanced in the β group. The levels of AM, its receptor and RAMPs are much less in BAT than in WAT but adrenergic stimulation has a greater effect on the AM and its receptor components in BAT than those in WAT. AM stimulates lipolysis and increases the level of uncoupling protein-1 (UCP-1) in BAT. It may therefore enhance thermogenesis by increasing the availability of free fatty acids substrate as well as the UCP-1 level on the mitochondrial membrane. © 2006 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/peptidesen_US
dc.relation.ispartofPeptidesen_US
dc.rightsPeptides. Copyright © Elsevier BV.-
dc.subject.meshAdipose Tissue, Brown - Chemistry - Drug Effects - Metabolismen_US
dc.subject.meshAdipose Tissue, White - Chemistry - Drug Effects - Metabolismen_US
dc.subject.meshAdrenergic Agents - Administration & Dosage - Pharmacologyen_US
dc.subject.meshAdrenomedullin - Genetics - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshBody Weight - Drug Effectsen_US
dc.subject.meshCalcitonin Receptor-Like Proteinen_US
dc.subject.meshGene Expression Regulation - Drug Effectsen_US
dc.subject.meshInjections, Subcutaneousen_US
dc.subject.meshIntracellular Signaling Peptides And Proteins - Genetics - Metabolismen_US
dc.subject.meshIon Channels - Metabolismen_US
dc.subject.meshIsoproterenol - Administration & Dosage - Pharmacologyen_US
dc.subject.meshLipolysis - Drug Effectsen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Proteins - Genetics - Metabolismen_US
dc.subject.meshMitochondrial Proteins - Metabolismen_US
dc.subject.meshPhenylephrine - Administration & Dosage - Pharmacologyen_US
dc.subject.meshProtein Precursors - Genetics - Metabolismen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshRadioimmunoassayen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptor Activity-Modifying Proteinsen_US
dc.subject.meshReceptors, Adrenomedullinen_US
dc.subject.meshReceptors, Calcitonin - Genetics - Metabolismen_US
dc.subject.meshReceptors, Peptide - Genetics - Metabolismen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.titleAdrenomedullin and its receptor components in adipose tissues: Differences between white and brown fats and the effects of adrenergic stimulationen_US
dc.typeArticleen_US
dc.identifier.emailTang, F:ftang@hkucc.hku.hken_US
dc.identifier.emailHwang, ISS: isabelbelbel@hotmail.com-
dc.identifier.authorityTang, F=rp00327en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.peptides.2006.12.007en_US
dc.identifier.pmid17250927-
dc.identifier.scopuseid_2-s2.0-33847668023en_US
dc.identifier.hkuros136960-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33847668023&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume28en_US
dc.identifier.issue4en_US
dc.identifier.spage920en_US
dc.identifier.epage927en_US
dc.identifier.isiWOS:000245625900025-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridGo, AGG=12040738300en_US
dc.identifier.scopusauthoridChow, KHM=26430472800en_US
dc.identifier.scopusauthoridHwang, ISS=7201615103en_US
dc.identifier.scopusauthoridTang, F=7201979770en_US

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