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Article: Relationship between muscarinic receptor reserve and mode of excitation-contraction coupling in airway smooth muscle

TitleRelationship between muscarinic receptor reserve and mode of excitation-contraction coupling in airway smooth muscle
Authors
Issue Date1997
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
Citation
Faseb Journal, 1997, v. 11 n. 3, p. A59 How to Cite?
AbstractAcetytcholine-induced contractile response in airway smooth muscle is mediated predominantly via the stimulation of M3 muscarinic receptor subtype The mode of coupling between this stimulation and muscle contraction is pharmacomechanical, i.e. independent of membrane potential by opposition to an electromechanical mode which is membrane potential dependent. We have postulated that muscarinic receptor reserve for contraction induced by agonist controls the mode of excitation-contraction coupling in airway smooth muscle. A fraction of the muscarinic receptor was inactivated with phenoxybenzamine (PBZ), and the relaxing effect of cromakalim (a K+ channel opener) on ACh-induced tonic contraction was determined in those conditions as an index for electromechanical coupling. Quantification of the decrease in receptor density following alkylation of muscarinic receptors by PBZ was confirmed using radioligand binding experiment. The results showed that maximal responses to ACh could still be elicited when functional receptors were reduced to 50 % following the treatment of PBZ (3 μM), indicating the presence of a large receptor reserve for ACh However, the effect of cromakalim was significantly increased following the diminution of muscarinic receptor as compare to the control (p < 0 01, n = 6) Therefore, reduction of muscarinic receptor reserve can switch the excitation-contraction coupling mode from pharmacomechanical to electromechanical coupling.
Persistent Identifierhttp://hdl.handle.net/10722/171749
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorShen, Sen_US
dc.contributor.authorBourreau, JPen_US
dc.date.accessioned2012-10-30T06:16:47Z-
dc.date.available2012-10-30T06:16:47Z-
dc.date.issued1997en_US
dc.identifier.citationFaseb Journal, 1997, v. 11 n. 3, p. A59en_US
dc.identifier.issn0892-6638en_US
dc.identifier.urihttp://hdl.handle.net/10722/171749-
dc.description.abstractAcetytcholine-induced contractile response in airway smooth muscle is mediated predominantly via the stimulation of M3 muscarinic receptor subtype The mode of coupling between this stimulation and muscle contraction is pharmacomechanical, i.e. independent of membrane potential by opposition to an electromechanical mode which is membrane potential dependent. We have postulated that muscarinic receptor reserve for contraction induced by agonist controls the mode of excitation-contraction coupling in airway smooth muscle. A fraction of the muscarinic receptor was inactivated with phenoxybenzamine (PBZ), and the relaxing effect of cromakalim (a K+ channel opener) on ACh-induced tonic contraction was determined in those conditions as an index for electromechanical coupling. Quantification of the decrease in receptor density following alkylation of muscarinic receptors by PBZ was confirmed using radioligand binding experiment. The results showed that maximal responses to ACh could still be elicited when functional receptors were reduced to 50 % following the treatment of PBZ (3 μM), indicating the presence of a large receptor reserve for ACh However, the effect of cromakalim was significantly increased following the diminution of muscarinic receptor as compare to the control (p < 0 01, n = 6) Therefore, reduction of muscarinic receptor reserve can switch the excitation-contraction coupling mode from pharmacomechanical to electromechanical coupling.en_US
dc.languageengen_US
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/en_US
dc.relation.ispartofFASEB Journalen_US
dc.titleRelationship between muscarinic receptor reserve and mode of excitation-contraction coupling in airway smooth muscleen_US
dc.typeArticleen_US
dc.identifier.emailBourreau, JP:bourreau@hkucc.hku.hken_US
dc.identifier.authorityBourreau, JP=rp00389en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-33750142763en_US
dc.identifier.hkuros25754-
dc.identifier.volume11en_US
dc.identifier.issue3en_US
dc.identifier.spageA59en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridShen, S=7403431355en_US
dc.identifier.scopusauthoridBourreau, JP=7003927886en_US

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