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Article: B-Raf and C-Raf are required for Ras-stimulated p42 MAP kinase activation in Xenopus egg extracts

TitleB-Raf and C-Raf are required for Ras-stimulated p42 MAP kinase activation in Xenopus egg extracts
Authors
Issue Date2006
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/onc
Citation
Oncogene, 2006, v. 25 n. 23, p. 3307-3315 How to Cite?
AbstractDuring mitosis, a select pool of MEK1 and p42/p44 MAPK becomes activated at the kinetochores and spindle poles, without substantial activation of the bulk of the cytoplasmic p42/p44 MAPK. Recently, we set out to identify the MAP kinase kinase kinase (MAPKKK) responsible for this mitotic activation, using cyclin-treated Xenopus egg extracts as a model system, and presented evidence that Mos was the relevant MAPKKK . However, a second MAPKKK distinct from Mos was readily detectable as well. Here, we partially purify this second MAPKKK and identify it as B-Raf. No changes in the activity of B-Raf were detectable during progesterone-induced oocyte maturation, after egg fertilization, or during the early embryonic cell cycle, arguing against a role for B-Raf in the mitotic activation of MEK1 and p42 MAPK. Ras proteins can bring about activation of MEK1 and p42 MAPK in extracts, and Ras may contribute to signaling from the classical progesterone receptor during oocyte maturation and from receptor tyrosine kinases during early embryogenesis. We found that both B-Raf and C-Raf, but not Mos, are required for Ras-induced MEK1 and p42 MAPK activation. These data indicate that two upstream stimuli, active Ras and active Cdc2, utilize different MAPKKKs to activate MEK1 and p42 MAPK. © 2006 Nature Publishing Group All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/171743
ISSN
2015 Impact Factor: 7.932
2015 SCImago Journal Rankings: 4.047
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYue, Jen_US
dc.contributor.authorXiong, Wen_US
dc.contributor.authorFerrell Jr, JEen_US
dc.date.accessioned2012-10-30T06:16:44Z-
dc.date.available2012-10-30T06:16:44Z-
dc.date.issued2006en_US
dc.identifier.citationOncogene, 2006, v. 25 n. 23, p. 3307-3315en_US
dc.identifier.issn0950-9232en_US
dc.identifier.urihttp://hdl.handle.net/10722/171743-
dc.description.abstractDuring mitosis, a select pool of MEK1 and p42/p44 MAPK becomes activated at the kinetochores and spindle poles, without substantial activation of the bulk of the cytoplasmic p42/p44 MAPK. Recently, we set out to identify the MAP kinase kinase kinase (MAPKKK) responsible for this mitotic activation, using cyclin-treated Xenopus egg extracts as a model system, and presented evidence that Mos was the relevant MAPKKK . However, a second MAPKKK distinct from Mos was readily detectable as well. Here, we partially purify this second MAPKKK and identify it as B-Raf. No changes in the activity of B-Raf were detectable during progesterone-induced oocyte maturation, after egg fertilization, or during the early embryonic cell cycle, arguing against a role for B-Raf in the mitotic activation of MEK1 and p42 MAPK. Ras proteins can bring about activation of MEK1 and p42 MAPK in extracts, and Ras may contribute to signaling from the classical progesterone receptor during oocyte maturation and from receptor tyrosine kinases during early embryogenesis. We found that both B-Raf and C-Raf, but not Mos, are required for Ras-induced MEK1 and p42 MAPK activation. These data indicate that two upstream stimuli, active Ras and active Cdc2, utilize different MAPKKKs to activate MEK1 and p42 MAPK. © 2006 Nature Publishing Group All rights reserved.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/oncen_US
dc.relation.ispartofOncogeneen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Extractsen_US
dc.subject.meshEnzyme Activation - Physiologyen_US
dc.subject.meshMap Kinase Kinase 1 - Metabolismen_US
dc.subject.meshMitogen-Activated Protein Kinase 1 - Metabolismen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOvum - Enzymologyen_US
dc.subject.meshProto-Oncogene Proteins B-Raf - Physiologyen_US
dc.subject.meshProto-Oncogene Proteins C-Raf - Physiologyen_US
dc.subject.meshXenopus Proteins - Metabolismen_US
dc.subject.meshXenopus Laevisen_US
dc.subject.meshRas Proteins - Physiologyen_US
dc.titleB-Raf and C-Raf are required for Ras-stimulated p42 MAP kinase activation in Xenopus egg extractsen_US
dc.typeArticleen_US
dc.identifier.emailYue, J:jyue@hku.hken_US
dc.identifier.authorityYue, J=rp00286en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/sj.onc.1209354en_US
dc.identifier.pmid16434971-
dc.identifier.scopuseid_2-s2.0-33744934562en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33744934562&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume25en_US
dc.identifier.issue23en_US
dc.identifier.spage3307en_US
dc.identifier.epage3315en_US
dc.identifier.isiWOS:000237951200009-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridYue, J=7101875828en_US
dc.identifier.scopusauthoridXiong, W=34874035600en_US
dc.identifier.scopusauthoridFerrell Jr, JE=7005371587en_US
dc.identifier.citeulike480154-

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