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Article: Identification of brain-derived neurotrophic factor in nestin-expressing astroglial cells in the neostriatum of 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine-treated mice

TitleIdentification of brain-derived neurotrophic factor in nestin-expressing astroglial cells in the neostriatum of 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine-treated mice
Authors
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
Citation
Neuroscience, 2004, v. 126 n. 4, p. 941-953 How to Cite?
AbstractUp-regulation of nestin expression was significantly induced in the caudate-putamen of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice in our previous observation [Brain Res 925 (2002) 9]. We hypothesized that the nestin-expressing cells might play an important role in the pathogenesis of parkinsonian model, and characterization of these nestin-expressing cells was studied by RT-PCR, immunohistochemistry and semi-quantitative analysis for various markers of glial fibrillary acid protein (GFAP), S-100, neuronal nuclear specific protein (NeuN), β-tubulin, Ki-67 and brain-derived neurotrophic factor (BDNF) expression in MPTP-treated C57/BL mice. Firstly, significant increasing in both nestin protein and mRNA was found in MPTP-treated mice. Up-regulation of nestin expression started at day 1, peaked at day 3, and gradually went down at days 7-21 in the neostriatum after MPTP treatment. Secondly, double immunofluorescence indicated that almost all of nestin-positive cells exhibited GFAP (98%) or S-100 (96%)-immunoreactivity, whereas NeuN or β-tubulin was hardly detected in these nestin-positive cells. Thirdly, a minor population (7.0%) of nestin-positive cells showed Ki-67 (cell proliferation marker)-immunoreactivity, showing some of them went into cell mitotic state. Finally but more interestingly, a major population (86%) of nestin-expressing cells also exhibited immunoreactivity for BDNF, one neurotrophic factor. These results present time-dependent up-regulation of nestin expression in neostriatum, the proliferative and neurotrophic properties of nestin-expressing astroglial cells in MPTP-treated C57/BL mice. Taken together with previous observations, this study suggests that nestin-expressing activated astroglial cells, possibly partially through synthesizing and releasing neurotrophic factors such as BDNF in the basal ganglia, may play important roles in protection of nigrostriatal dopamine neurons and in the pathogenesis of Parkinson's disease in mammals. © 2004 IBRO. Published by Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/171741
ISSN
2015 Impact Factor: 3.231
2015 SCImago Journal Rankings: 1.768
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, LWen_US
dc.contributor.authorHu, HJen_US
dc.contributor.authorLiu, HLen_US
dc.contributor.authorYung, KKLen_US
dc.contributor.authorChan, YSen_US
dc.date.accessioned2012-10-30T06:16:43Z-
dc.date.available2012-10-30T06:16:43Z-
dc.date.issued2004en_US
dc.identifier.citationNeuroscience, 2004, v. 126 n. 4, p. 941-953en_US
dc.identifier.issn0306-4522en_US
dc.identifier.urihttp://hdl.handle.net/10722/171741-
dc.description.abstractUp-regulation of nestin expression was significantly induced in the caudate-putamen of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice in our previous observation [Brain Res 925 (2002) 9]. We hypothesized that the nestin-expressing cells might play an important role in the pathogenesis of parkinsonian model, and characterization of these nestin-expressing cells was studied by RT-PCR, immunohistochemistry and semi-quantitative analysis for various markers of glial fibrillary acid protein (GFAP), S-100, neuronal nuclear specific protein (NeuN), β-tubulin, Ki-67 and brain-derived neurotrophic factor (BDNF) expression in MPTP-treated C57/BL mice. Firstly, significant increasing in both nestin protein and mRNA was found in MPTP-treated mice. Up-regulation of nestin expression started at day 1, peaked at day 3, and gradually went down at days 7-21 in the neostriatum after MPTP treatment. Secondly, double immunofluorescence indicated that almost all of nestin-positive cells exhibited GFAP (98%) or S-100 (96%)-immunoreactivity, whereas NeuN or β-tubulin was hardly detected in these nestin-positive cells. Thirdly, a minor population (7.0%) of nestin-positive cells showed Ki-67 (cell proliferation marker)-immunoreactivity, showing some of them went into cell mitotic state. Finally but more interestingly, a major population (86%) of nestin-expressing cells also exhibited immunoreactivity for BDNF, one neurotrophic factor. These results present time-dependent up-regulation of nestin expression in neostriatum, the proliferative and neurotrophic properties of nestin-expressing astroglial cells in MPTP-treated C57/BL mice. Taken together with previous observations, this study suggests that nestin-expressing activated astroglial cells, possibly partially through synthesizing and releasing neurotrophic factors such as BDNF in the basal ganglia, may play important roles in protection of nigrostriatal dopamine neurons and in the pathogenesis of Parkinson's disease in mammals. © 2004 IBRO. Published by Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscienceen_US
dc.relation.ispartofNeuroscienceen_US
dc.subject.mesh1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAstrocytes - Drug Effects - Metabolismen_US
dc.subject.meshBlotting, Northern - Methodsen_US
dc.subject.meshBrain-Derived Neurotrophic Factor - Isolation & Purificationen_US
dc.subject.meshCell Count - Methodsen_US
dc.subject.meshDopamine Agents - Pharmacologyen_US
dc.subject.meshGene Expression Regulation - Drug Effectsen_US
dc.subject.meshGlial Fibrillary Acidic Protein - Metabolismen_US
dc.subject.meshImmunohistochemistry - Methodsen_US
dc.subject.meshIntermediate Filament Proteins - Genetics - Metabolismen_US
dc.subject.meshKi-67 Antigen - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshNeostriatum - Cytology - Drug Effectsen_US
dc.subject.meshNerve Tissue Proteins - Genetics - Metabolismen_US
dc.subject.meshS100 Proteins - Metabolismen_US
dc.subject.meshTime Factorsen_US
dc.titleIdentification of brain-derived neurotrophic factor in nestin-expressing astroglial cells in the neostriatum of 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine-treated miceen_US
dc.typeArticleen_US
dc.identifier.emailChan, YS:yschan@hkucc.hku.hken_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.neuroscience.2004.04.020en_US
dc.identifier.pmid15207328-
dc.identifier.scopuseid_2-s2.0-2942642117en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2942642117&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume126en_US
dc.identifier.issue4en_US
dc.identifier.spage941en_US
dc.identifier.epage953en_US
dc.identifier.isiWOS:000222552900013-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChen, LW=7409444941en_US
dc.identifier.scopusauthoridHu, HJ=8683277000en_US
dc.identifier.scopusauthoridLiu, HL=36066506000en_US
dc.identifier.scopusauthoridYung, KKL=13605496000en_US
dc.identifier.scopusauthoridChan, YS=7403676627en_US

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