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Article: Differential Expression of α-Amino-3-Hydroxy-5-Methyl-4-Isoxazole- Propionate Glutamate Receptors in the Rat Striatum during Postnatal Development

TitleDifferential Expression of α-Amino-3-Hydroxy-5-Methyl-4-Isoxazole- Propionate Glutamate Receptors in the Rat Striatum during Postnatal Development
Authors
Issue Date2003
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
Neurosignals, 2003, v. 12 n. 6, p. 302-309 How to Cite?
AbstractAlpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA)-type glutamate receptors (GluR1-4) are one of the most important ionotropic glutamate receptors in the striatum, a key region of the basal ganglia. The present study investigated the trend of developmental expression of AMPA receptor subunits in the striatum of rats in different developmental stages, i.e., at postnatal day 7 (PND7), PND21 and adult. Perfuse-fixed striatal sections were used. The expression of AMPA subunits was studied by immunofluorescence and reverse transcriptase-polymerase chain reaction (RT-PCR). RT-PCR revealed that the levels of expression of the GluR1 and GluR3 mRNAs were the same among the age groups. The level of GluR2 mRNA was highest in PND21 rats and lowest in adult. The highest level of GluR4 mRNA was detected in rats at PND7. Similar trends of GluR1, GluR2 and GluR2/3 immunoreactivity expression were observed using commercially available specific antibodies. In addition, a large proportion of parvalbumin-immunoreactive GABAergic interneurons in the striatum were found to display GluR1 immunoreactivity in PND21 and adult rats. In contrast, most of the choline acetyltransferase- immunoreactive cholinergic interneurons were found to display GluR2 immunoreactivity but less GluR1 and no GluR2/3 immunoreactivity in PND21 and adult rats. The present study suggests that there is a distinct pattern of expression of AMPA-type receptor mRNAs and proteins in the rat striatum at different stages of development. Copyright © 2003 S. Karger AG, Basel.
Persistent Identifierhttp://hdl.handle.net/10722/171725
ISSN
2015 Impact Factor: 1.593
2015 SCImago Journal Rankings: 0.763
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, WSen_US
dc.contributor.authorYeung, CWen_US
dc.contributor.authorChung, EKYen_US
dc.contributor.authorLau, WKen_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorYung, KKLen_US
dc.date.accessioned2012-10-30T06:16:37Z-
dc.date.available2012-10-30T06:16:37Z-
dc.date.issued2003en_US
dc.identifier.citationNeurosignals, 2003, v. 12 n. 6, p. 302-309en_US
dc.identifier.issn1424-862Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/171725-
dc.description.abstractAlpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA)-type glutamate receptors (GluR1-4) are one of the most important ionotropic glutamate receptors in the striatum, a key region of the basal ganglia. The present study investigated the trend of developmental expression of AMPA receptor subunits in the striatum of rats in different developmental stages, i.e., at postnatal day 7 (PND7), PND21 and adult. Perfuse-fixed striatal sections were used. The expression of AMPA subunits was studied by immunofluorescence and reverse transcriptase-polymerase chain reaction (RT-PCR). RT-PCR revealed that the levels of expression of the GluR1 and GluR3 mRNAs were the same among the age groups. The level of GluR2 mRNA was highest in PND21 rats and lowest in adult. The highest level of GluR4 mRNA was detected in rats at PND7. Similar trends of GluR1, GluR2 and GluR2/3 immunoreactivity expression were observed using commercially available specific antibodies. In addition, a large proportion of parvalbumin-immunoreactive GABAergic interneurons in the striatum were found to display GluR1 immunoreactivity in PND21 and adult rats. In contrast, most of the choline acetyltransferase- immunoreactive cholinergic interneurons were found to display GluR2 immunoreactivity but less GluR1 and no GluR2/3 immunoreactivity in PND21 and adult rats. The present study suggests that there is a distinct pattern of expression of AMPA-type receptor mRNAs and proteins in the rat striatum at different stages of development. Copyright © 2003 S. Karger AG, Basel.en_US
dc.languageengen_US
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSGen_US
dc.relation.ispartofNeuroSignalsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshCholine O-Acetyltransferase - Metabolismen_US
dc.subject.meshFluorescent Antibody Technique - Methodsen_US
dc.subject.meshGene Expression Regulation, Developmental - Physiologyen_US
dc.subject.meshNeostriatum - Growth & Development - Metabolismen_US
dc.subject.meshParvalbumins - Metabolismen_US
dc.subject.meshRna, Messenger - Biosynthesisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptors, Ampa - Genetics - Metabolismen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - Methodsen_US
dc.titleDifferential Expression of α-Amino-3-Hydroxy-5-Methyl-4-Isoxazole- Propionate Glutamate Receptors in the Rat Striatum during Postnatal Developmenten_US
dc.typeArticleen_US
dc.identifier.emailChan, YS:yschan@hkucc.hku.hken_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1159/000075312en_US
dc.identifier.pmid14739560-
dc.identifier.scopuseid_2-s2.0-0442324648en_US
dc.identifier.hkuros92120-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0442324648&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume12en_US
dc.identifier.issue6en_US
dc.identifier.spage302en_US
dc.identifier.epage309en_US
dc.identifier.isiWOS:000188326400004-
dc.publisher.placeSwitzerlanden_US
dc.identifier.scopusauthoridChan, WS=7403918312en_US
dc.identifier.scopusauthoridYeung, CW=36848789000en_US
dc.identifier.scopusauthoridChung, EKY=16315234600en_US
dc.identifier.scopusauthoridLau, WK=7402933189en_US
dc.identifier.scopusauthoridChan, YS=7403676627en_US
dc.identifier.scopusauthoridYung, KKL=13605496000en_US

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