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Article: The Inhibitory Effect of Adrenomedullin in the Rat Ileum: Cross-Talk with β3-Adrenoceptor in the Serotonin-Induced Muscle Contraction

TitleThe Inhibitory Effect of Adrenomedullin in the Rat Ileum: Cross-Talk with β3-Adrenoceptor in the Serotonin-Induced Muscle Contraction
Authors
Issue Date2004
PublisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.org
Citation
Journal Of Pharmacology And Experimental Therapeutics, 2004, v. 308 n. 1, p. 241-248 How to Cite?
AbstractIn contrast to vascular muscles, the contribution of a hypotensive peptide adrenomedullin (AM) to the regulation of visceral smooth muscles is obscure. The content, synthesis, and effects of AM on the muscular tone in rat ileum were explored. It was found that there was immunoreactive AM (301 pg/mg of protein) and AM mRNA expression (162 fg/pg actin mRNA) in the ileum and that AM evoked relaxation in ileal strips (Ki = 0.85 nM) precontracted with serotonin. Antagonists of both AM (AM22-52) and calcitonin gene-related peptide (CGRP8-37) receptors did not affect this AM-induced relaxation, whereas it was suppressed by a selective blocker of β3-adrenoreceptor (SR 59230A). The AM-induced relaxation was accompanied by a production of cAMP. Antagonists of protein kinases A (KT 5720 and H-7) and an inhibitor of the ATP-dependent K+-channels (glibenclamide) attenuated the effect of AM. We suggest that AM is a local regulator of the ileal tone, with an inhibitory action on muscle contraction. AM may activate the β3-adrenoceptors, resulting in protein kinase A activation, which in turn opens the ATP-dependent K +-channels.
Persistent Identifierhttp://hdl.handle.net/10722/171724
ISSN
2015 Impact Factor: 3.76
2015 SCImago Journal Rankings: 1.847
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKravtsov, GMen_US
dc.contributor.authorHwang, ISSen_US
dc.contributor.authorTang, Fen_US
dc.date.accessioned2012-10-30T06:16:37Z-
dc.date.available2012-10-30T06:16:37Z-
dc.date.issued2004en_US
dc.identifier.citationJournal Of Pharmacology And Experimental Therapeutics, 2004, v. 308 n. 1, p. 241-248en_US
dc.identifier.issn0022-3565en_US
dc.identifier.urihttp://hdl.handle.net/10722/171724-
dc.description.abstractIn contrast to vascular muscles, the contribution of a hypotensive peptide adrenomedullin (AM) to the regulation of visceral smooth muscles is obscure. The content, synthesis, and effects of AM on the muscular tone in rat ileum were explored. It was found that there was immunoreactive AM (301 pg/mg of protein) and AM mRNA expression (162 fg/pg actin mRNA) in the ileum and that AM evoked relaxation in ileal strips (Ki = 0.85 nM) precontracted with serotonin. Antagonists of both AM (AM22-52) and calcitonin gene-related peptide (CGRP8-37) receptors did not affect this AM-induced relaxation, whereas it was suppressed by a selective blocker of β3-adrenoreceptor (SR 59230A). The AM-induced relaxation was accompanied by a production of cAMP. Antagonists of protein kinases A (KT 5720 and H-7) and an inhibitor of the ATP-dependent K+-channels (glibenclamide) attenuated the effect of AM. We suggest that AM is a local regulator of the ileal tone, with an inhibitory action on muscle contraction. AM may activate the β3-adrenoceptors, resulting in protein kinase A activation, which in turn opens the ATP-dependent K +-channels.en_US
dc.languageengen_US
dc.publisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.orgen_US
dc.relation.ispartofJournal of Pharmacology and Experimental Therapeuticsen_US
dc.subject.meshAdrenomedullinen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCyclic Amp - Metabolismen_US
dc.subject.meshCyclic Amp-Dependent Protein Kinases - Metabolismen_US
dc.subject.meshEthanolamines - Pharmacologyen_US
dc.subject.meshIleum - Metabolismen_US
dc.subject.meshMuscle Contraction - Drug Effectsen_US
dc.subject.meshMuscle, Smooth - Drug Effects - Physiologyen_US
dc.subject.meshNitric Oxide - Metabolismen_US
dc.subject.meshPeptides - Genetics - Metabolism - Pharmacologyen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshReceptor Cross-Talk - Physiologyen_US
dc.subject.meshReceptors, Adrenergic, Beta-3 - Metabolismen_US
dc.subject.meshSerotonin - Pharmacologyen_US
dc.titleThe Inhibitory Effect of Adrenomedullin in the Rat Ileum: Cross-Talk with β3-Adrenoceptor in the Serotonin-Induced Muscle Contractionen_US
dc.typeArticleen_US
dc.identifier.emailTang, F:ftang@hkucc.hku.hken_US
dc.identifier.authorityTang, F=rp00327en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1124/jpet.103.057612en_US
dc.identifier.pmid14569072-
dc.identifier.scopuseid_2-s2.0-0345866804en_US
dc.identifier.hkuros88489-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0345866804&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume308en_US
dc.identifier.issue1en_US
dc.identifier.spage241en_US
dc.identifier.epage248en_US
dc.identifier.isiWOS:000187888500032-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKravtsov, GM=7003811092en_US
dc.identifier.scopusauthoridHwang, ISS=7201615103en_US
dc.identifier.scopusauthoridTang, F=7201979770en_US

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