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- Publisher Website: 10.1006/prep.1997.0811
- Scopus: eid_2-s2.0-0345698969
- PMID: 9473467
- WOS: WOS:000072119000018
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Article: Functional expression of secreted mouse BST-1 in yeast
Title | Functional expression of secreted mouse BST-1 in yeast |
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Authors | |
Issue Date | 1998 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yprep |
Citation | Protein Expression And Purification, 1998, v. 12 n. 1, p. 133-137 How to Cite? |
Abstract | BST-1, a bone marrow stromal cell surface antigen, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity. The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores. The expression and characterization of human BST-1 in certain mammalian cell lines have been reported. We have expressed the murine BST-1 in yeast as a 6 x His-tagged secreted protein. The recombinant protein has been purified and subjected to structural and functional characterization. It has an apparent molecular mass of 38.5 kDa on SDS-PAGE gel stained with Coomassie blue and is recognized on Western blots by a rabbit polyclonal antibody against BST-1. Deglycosylation of the protein with N-glycanase produces a ladder of bands with molecular sizes ranging from 32 to 39 kDa. The protein possesses the ADP-ribosyl cyclase activity as measured using nicotinamide guanine dinucleotide as substrate. |
Persistent Identifier | http://hdl.handle.net/10722/171723 |
ISSN | 2023 Impact Factor: 1.4 2023 SCImago Journal Rankings: 0.383 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hussain, AMM | en_US |
dc.contributor.author | Lee, HC | en_US |
dc.contributor.author | Chang, CF | en_US |
dc.date.accessioned | 2012-10-30T06:16:37Z | - |
dc.date.available | 2012-10-30T06:16:37Z | - |
dc.date.issued | 1998 | en_US |
dc.identifier.citation | Protein Expression And Purification, 1998, v. 12 n. 1, p. 133-137 | en_US |
dc.identifier.issn | 1046-5928 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171723 | - |
dc.description.abstract | BST-1, a bone marrow stromal cell surface antigen, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity. The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores. The expression and characterization of human BST-1 in certain mammalian cell lines have been reported. We have expressed the murine BST-1 in yeast as a 6 x His-tagged secreted protein. The recombinant protein has been purified and subjected to structural and functional characterization. It has an apparent molecular mass of 38.5 kDa on SDS-PAGE gel stained with Coomassie blue and is recognized on Western blots by a rabbit polyclonal antibody against BST-1. Deglycosylation of the protein with N-glycanase produces a ladder of bands with molecular sizes ranging from 32 to 39 kDa. The protein possesses the ADP-ribosyl cyclase activity as measured using nicotinamide guanine dinucleotide as substrate. | en_US |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yprep | en_US |
dc.relation.ispartof | Protein Expression and Purification | en_US |
dc.subject.mesh | Adp-Ribosyl Cyclase | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antigens, Cd | en_US |
dc.subject.mesh | Antigens, Surface - Chemistry - Genetics - Isolation & Purification | en_US |
dc.subject.mesh | Cloning, Molecular | en_US |
dc.subject.mesh | Gpi-Linked Proteins | en_US |
dc.subject.mesh | Gene Expression | en_US |
dc.subject.mesh | Histidine - Chemistry | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Immunochemistry | en_US |
dc.subject.mesh | Membrane Glycoproteins - Genetics - Isolation & Purification - Secretion | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Molecular Weight | en_US |
dc.subject.mesh | Rabbits | en_US |
dc.subject.mesh | Recombinant Proteins - Chemistry - Genetics - Isolation & Purification | en_US |
dc.subject.mesh | Saccharomyces Cerevisiae - Genetics | en_US |
dc.title | Functional expression of secreted mouse BST-1 in yeast | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lee, HC:leehc@hku.hk | en_US |
dc.identifier.authority | Lee, HC=rp00545 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1006/prep.1997.0811 | en_US |
dc.identifier.pmid | 9473467 | - |
dc.identifier.scopus | eid_2-s2.0-0345698969 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0345698969&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 12 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 133 | en_US |
dc.identifier.epage | 137 | en_US |
dc.identifier.isi | WOS:000072119000018 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Hussain, AMM=9733025800 | en_US |
dc.identifier.scopusauthorid | Lee, HC=26642959100 | en_US |
dc.identifier.scopusauthorid | Chang, CF=7407035747 | en_US |
dc.identifier.issnl | 1046-5928 | - |