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Article: Functional expression of secreted mouse BST-1 in yeast

TitleFunctional expression of secreted mouse BST-1 in yeast
Authors
Issue Date1998
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yprep
Citation
Protein Expression And Purification, 1998, v. 12 n. 1, p. 133-137 How to Cite?
AbstractBST-1, a bone marrow stromal cell surface antigen, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity. The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores. The expression and characterization of human BST-1 in certain mammalian cell lines have been reported. We have expressed the murine BST-1 in yeast as a 6 x His-tagged secreted protein. The recombinant protein has been purified and subjected to structural and functional characterization. It has an apparent molecular mass of 38.5 kDa on SDS-PAGE gel stained with Coomassie blue and is recognized on Western blots by a rabbit polyclonal antibody against BST-1. Deglycosylation of the protein with N-glycanase produces a ladder of bands with molecular sizes ranging from 32 to 39 kDa. The protein possesses the ADP-ribosyl cyclase activity as measured using nicotinamide guanine dinucleotide as substrate.
Persistent Identifierhttp://hdl.handle.net/10722/171723
ISSN
2015 Impact Factor: 1.407
2015 SCImago Journal Rankings: 0.767
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHussain, AMMen_US
dc.contributor.authorLee, HCen_US
dc.contributor.authorChang, CFen_US
dc.date.accessioned2012-10-30T06:16:37Z-
dc.date.available2012-10-30T06:16:37Z-
dc.date.issued1998en_US
dc.identifier.citationProtein Expression And Purification, 1998, v. 12 n. 1, p. 133-137en_US
dc.identifier.issn1046-5928en_US
dc.identifier.urihttp://hdl.handle.net/10722/171723-
dc.description.abstractBST-1, a bone marrow stromal cell surface antigen, is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity. The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores. The expression and characterization of human BST-1 in certain mammalian cell lines have been reported. We have expressed the murine BST-1 in yeast as a 6 x His-tagged secreted protein. The recombinant protein has been purified and subjected to structural and functional characterization. It has an apparent molecular mass of 38.5 kDa on SDS-PAGE gel stained with Coomassie blue and is recognized on Western blots by a rabbit polyclonal antibody against BST-1. Deglycosylation of the protein with N-glycanase produces a ladder of bands with molecular sizes ranging from 32 to 39 kDa. The protein possesses the ADP-ribosyl cyclase activity as measured using nicotinamide guanine dinucleotide as substrate.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yprepen_US
dc.relation.ispartofProtein Expression and Purificationen_US
dc.subject.meshAdp-Ribosyl Cyclaseen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Cden_US
dc.subject.meshAntigens, Surface - Chemistry - Genetics - Isolation & Purificationen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshGpi-Linked Proteinsen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshHistidine - Chemistryen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunochemistryen_US
dc.subject.meshMembrane Glycoproteins - Genetics - Isolation & Purification - Secretionen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshRabbitsen_US
dc.subject.meshRecombinant Proteins - Chemistry - Genetics - Isolation & Purificationen_US
dc.subject.meshSaccharomyces Cerevisiae - Geneticsen_US
dc.titleFunctional expression of secreted mouse BST-1 in yeasten_US
dc.typeArticleen_US
dc.identifier.emailLee, HC:leehc@hku.hken_US
dc.identifier.authorityLee, HC=rp00545en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/prep.1997.0811en_US
dc.identifier.pmid9473467-
dc.identifier.scopuseid_2-s2.0-0345698969en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0345698969&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume12en_US
dc.identifier.issue1en_US
dc.identifier.spage133en_US
dc.identifier.epage137en_US
dc.identifier.isiWOS:000072119000018-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHussain, AMM=9733025800en_US
dc.identifier.scopusauthoridLee, HC=26642959100en_US
dc.identifier.scopusauthoridChang, CF=7407035747en_US

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