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Article: Analysis of Differentially Expressed Genes in Hepatocellular Carcinoma with Hepatitis C Virus by Suppression Subtractive Hybridization

TitleAnalysis of Differentially Expressed Genes in Hepatocellular Carcinoma with Hepatitis C Virus by Suppression Subtractive Hybridization
Authors
KeywordsHepatitis C Virus
Hepatocellular Carcinoma
Suppression Subtractive Hybridization
Issue Date2002
Citation
Oncology Research, 2002, v. 13 n. 3, p. 161-167 How to Cite?
AbstractHepatitis C virus (HCV) infection is associated with pathogenesis of hepatocellular carcinoma (HCC). We carried out suppression subtractive hybridization to identify variable expression of genes linked to HCC with HCV infection. RNA from both tumorous (tester) and nontumorous (driver) liver tissues was isolated. The cDNA clones were subjected to MegaBACE PCR sequencing to identify those that hybridized to the subtracted library with preference. Nucleic acid sequences generated were searched against the human UniGene database. Among 576 clones screened in the tumorous liver tissue, we identified 30 genes and 28 expressed sequence tags (ESTs). Among 30 genes detected, 23 were with known functions and 7 with unknown functions. The known genes identified had diversified functions and could be divided into 10 functional categories. Twenty percent of these genes were previously known to be tumor related and those most frequently appearing were haptoglobin alpha(2FS)-beta precursor, haptoglobin related protein, and alpha-2-macroglobulin. Four out of 30 known genes (immunoglobulin lambda light chain, kappa immunoglobulin, spliceosomal protein, and X-ray repair cross-complementing protein) were related to chromosome translocation and nucleotide repair. These four genes may contribute to carcinogenesis caused by DNA-damaged agents and to the efficiency of anticancer therapy. The genes with unknown function, which were most frequently detected, were PRO2760 and PRO2955; both encode proteins that express in fetal liver. Twenty-one known and six novel genes were discovered in the nontumorous liver tissue. Apparently, these 27 genes were lost in the tumorous liver tissues. Therefore, using suppression subtractive hybridization, we have identified a number of genes associated with HCC with HCV infection. Most of these genes have not been reported in HCC. Further characterization of these differentially expressed known and unknown genes will provide useful information in understanding the genes responsible for the development of HCC.
Persistent Identifierhttp://hdl.handle.net/10722/171717
ISSN
2015 Impact Factor: 3.957
2015 SCImago Journal Rankings: 0.740
References
Errata

 

DC FieldValueLanguage
dc.contributor.authorKotaka, Men_US
dc.contributor.authorChen, GGen_US
dc.contributor.authorLai, PBSen_US
dc.contributor.authorLau, WYen_US
dc.contributor.authorChan, PKSen_US
dc.contributor.authorLeung, TWTen_US
dc.contributor.authorLi, AKCen_US
dc.date.accessioned2012-10-30T06:16:35Z-
dc.date.available2012-10-30T06:16:35Z-
dc.date.issued2002en_US
dc.identifier.citationOncology Research, 2002, v. 13 n. 3, p. 161-167en_US
dc.identifier.issn0965-0407en_US
dc.identifier.urihttp://hdl.handle.net/10722/171717-
dc.description.abstractHepatitis C virus (HCV) infection is associated with pathogenesis of hepatocellular carcinoma (HCC). We carried out suppression subtractive hybridization to identify variable expression of genes linked to HCC with HCV infection. RNA from both tumorous (tester) and nontumorous (driver) liver tissues was isolated. The cDNA clones were subjected to MegaBACE PCR sequencing to identify those that hybridized to the subtracted library with preference. Nucleic acid sequences generated were searched against the human UniGene database. Among 576 clones screened in the tumorous liver tissue, we identified 30 genes and 28 expressed sequence tags (ESTs). Among 30 genes detected, 23 were with known functions and 7 with unknown functions. The known genes identified had diversified functions and could be divided into 10 functional categories. Twenty percent of these genes were previously known to be tumor related and those most frequently appearing were haptoglobin alpha(2FS)-beta precursor, haptoglobin related protein, and alpha-2-macroglobulin. Four out of 30 known genes (immunoglobulin lambda light chain, kappa immunoglobulin, spliceosomal protein, and X-ray repair cross-complementing protein) were related to chromosome translocation and nucleotide repair. These four genes may contribute to carcinogenesis caused by DNA-damaged agents and to the efficiency of anticancer therapy. The genes with unknown function, which were most frequently detected, were PRO2760 and PRO2955; both encode proteins that express in fetal liver. Twenty-one known and six novel genes were discovered in the nontumorous liver tissue. Apparently, these 27 genes were lost in the tumorous liver tissues. Therefore, using suppression subtractive hybridization, we have identified a number of genes associated with HCC with HCV infection. Most of these genes have not been reported in HCC. Further characterization of these differentially expressed known and unknown genes will provide useful information in understanding the genes responsible for the development of HCC.en_US
dc.languageengen_US
dc.relation.ispartofOncology Researchen_US
dc.subjectHepatitis C Virusen_US
dc.subjectHepatocellular Carcinomaen_US
dc.subjectSuppression Subtractive Hybridizationen_US
dc.titleAnalysis of Differentially Expressed Genes in Hepatocellular Carcinoma with Hepatitis C Virus by Suppression Subtractive Hybridizationen_US
dc.typeArticleen_US
dc.identifier.emailKotaka, M:masayo@hku.hken_US
dc.identifier.authorityKotaka, M=rp00293en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0043231581en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0043231581&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume13en_US
dc.identifier.issue3en_US
dc.identifier.spage161en_US
dc.identifier.epage167en_US
dc.publisher.placeUnited Statesen_US
dc.relation.erratumeid:eid_2-s2.0-0842348954-
dc.identifier.scopusauthoridKotaka, M=6604073578en_US
dc.identifier.scopusauthoridChen, GG=35291566400en_US
dc.identifier.scopusauthoridLai, PBS=7202946421en_US
dc.identifier.scopusauthoridLau, WY=7402933199en_US
dc.identifier.scopusauthoridChan, PKS=7403497792en_US
dc.identifier.scopusauthoridLeung, TWT=16151388900en_US
dc.identifier.scopusauthoridLi, AKC=36985145300en_US

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