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- Publisher Website: 10.1152/japplphysiol.01128.2002
- Scopus: eid_2-s2.0-0038516864
- PMID: 12588791
- WOS: WOS:000182699800048
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Article: Intracellular sodium in mammalian muscle fibers after eccentric contractions
Title | Intracellular sodium in mammalian muscle fibers after eccentric contractions |
---|---|
Authors | |
Keywords | Eccentric damage Gadolinium Intracellular sodium Muscle Stretch-sensitive channels |
Issue Date | 2003 |
Citation | Journal Of Applied Physiology, 2003, v. 94 n. 6, p. 2475-2482 How to Cite? |
Abstract | The effect of eccentric contractions on intracellular Na+ concentration ([Na+]i) and its distribution were examined in isolated rat and mouse muscle fiber bundles. [Na+]i was measured with either Na+-binding benzofuran isophthalate or sodium green. Ten isometric contractions had no significant effect on force (measured after 5 min of recovery) and caused no significant change in the resting [Na+]i (7.2 ± 0.5 mM). In contrast 10 eccentric contractions (40% stretch at 4 muscle lengths/s) reduced developed force at 100 Hz to 45 ± 3% of control and increased [Na+]i to 16.3 ± 1.6 mM (n = 6; P < 0.001). The rise of [Na+]i occurred over 1-2 min and showed only minimal recovery after 30 min. Confocal images of the distribution of [Na+]i showed a spatially uniform distribution both at rest and after eccentric contractions. Gd3+ (20 μM) had no effect on resting [Na+]i or control tetanic force but prevented the rise of [Na+]i and reduced the force deficit after eccentric damage. These data suggest that Na+ entry after eccentric contractions may occur principally through stretch-sensitive channels. |
Persistent Identifier | http://hdl.handle.net/10722/171715 |
ISSN | 2023 Impact Factor: 3.3 2023 SCImago Journal Rankings: 1.042 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yeung, EW | en_US |
dc.contributor.author | Ballard, HJ | en_US |
dc.contributor.author | Bourreau, JP | en_US |
dc.contributor.author | Allen, DG | en_US |
dc.date.accessioned | 2012-10-30T06:16:34Z | - |
dc.date.available | 2012-10-30T06:16:34Z | - |
dc.date.issued | 2003 | en_US |
dc.identifier.citation | Journal Of Applied Physiology, 2003, v. 94 n. 6, p. 2475-2482 | en_US |
dc.identifier.issn | 8750-7587 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171715 | - |
dc.description.abstract | The effect of eccentric contractions on intracellular Na+ concentration ([Na+]i) and its distribution were examined in isolated rat and mouse muscle fiber bundles. [Na+]i was measured with either Na+-binding benzofuran isophthalate or sodium green. Ten isometric contractions had no significant effect on force (measured after 5 min of recovery) and caused no significant change in the resting [Na+]i (7.2 ± 0.5 mM). In contrast 10 eccentric contractions (40% stretch at 4 muscle lengths/s) reduced developed force at 100 Hz to 45 ± 3% of control and increased [Na+]i to 16.3 ± 1.6 mM (n = 6; P < 0.001). The rise of [Na+]i occurred over 1-2 min and showed only minimal recovery after 30 min. Confocal images of the distribution of [Na+]i showed a spatially uniform distribution both at rest and after eccentric contractions. Gd3+ (20 μM) had no effect on resting [Na+]i or control tetanic force but prevented the rise of [Na+]i and reduced the force deficit after eccentric damage. These data suggest that Na+ entry after eccentric contractions may occur principally through stretch-sensitive channels. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Journal of Applied Physiology | en_US |
dc.subject | Eccentric damage | - |
dc.subject | Gadolinium | - |
dc.subject | Intracellular sodium | - |
dc.subject | Muscle | - |
dc.subject | Stretch-sensitive channels | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Benzofurans | en_US |
dc.subject.mesh | Electric Stimulation | en_US |
dc.subject.mesh | Enzyme Inhibitors - Pharmacology | en_US |
dc.subject.mesh | Ethers, Cyclic | en_US |
dc.subject.mesh | Fluorescent Dyes | en_US |
dc.subject.mesh | Gadolinium - Pharmacology | en_US |
dc.subject.mesh | Intracellular Membranes - Metabolism | en_US |
dc.subject.mesh | Ion Channels - Antagonists & Inhibitors - Metabolism | en_US |
dc.subject.mesh | Isometric Contraction - Drug Effects - Physiology | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Microscopy, Confocal | en_US |
dc.subject.mesh | Muscle Contraction - Drug Effects - Physiology | en_US |
dc.subject.mesh | Muscle Fibers, Skeletal - Metabolism | en_US |
dc.subject.mesh | Muscle, Skeletal - Physiology | en_US |
dc.subject.mesh | Organic Chemicals | en_US |
dc.subject.mesh | Osmolar Concentration | en_US |
dc.subject.mesh | Ouabain - Pharmacology | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Sodium - Metabolism | en_US |
dc.subject.mesh | Sodium-Potassium-Exchanging Atpase - Antagonists & Inhibitors | en_US |
dc.subject.mesh | Tissue Distribution | en_US |
dc.title | Intracellular sodium in mammalian muscle fibers after eccentric contractions | en_US |
dc.type | Article | en_US |
dc.identifier.email | Ballard, HJ:ballard@hkucc.hku.hk | en_US |
dc.identifier.email | Bourreau, JP:bourreau@hkucc.hku.hk | en_US |
dc.identifier.authority | Ballard, HJ=rp00367 | en_US |
dc.identifier.authority | Bourreau, JP=rp00389 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1152/japplphysiol.01128.2002 | - |
dc.identifier.pmid | 12588791 | - |
dc.identifier.scopus | eid_2-s2.0-0038516864 | en_US |
dc.identifier.hkuros | 82589 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0038516864&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 94 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | 2475 | en_US |
dc.identifier.epage | 2482 | en_US |
dc.identifier.isi | WOS:000182699800048 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Yeung, EW=7101971748 | en_US |
dc.identifier.scopusauthorid | Ballard, HJ=7005286310 | en_US |
dc.identifier.scopusauthorid | Bourreau, JP=7003927886 | en_US |
dc.identifier.scopusauthorid | Allen, DG=35509336700 | en_US |
dc.identifier.issnl | 1522-1601 | - |