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Article: Intracellular sodium in mammalian muscle fibers after eccentric contractions

TitleIntracellular sodium in mammalian muscle fibers after eccentric contractions
Authors
Issue Date2003
Citation
Journal Of Applied Physiology, 2003, v. 94 n. 6, p. 2475-2482 How to Cite?
AbstractThe effect of eccentric contractions on intracellular Na+ concentration ([Na+]i) and its distribution were examined in isolated rat and mouse muscle fiber bundles. [Na+]i was measured with either Na+-binding benzofuran isophthalate or sodium green. Ten isometric contractions had no significant effect on force (measured after 5 min of recovery) and caused no significant change in the resting [Na+]i (7.2 ± 0.5 mM). In contrast 10 eccentric contractions (40% stretch at 4 muscle lengths/s) reduced developed force at 100 Hz to 45 ± 3% of control and increased [Na+]i to 16.3 ± 1.6 mM (n = 6; P < 0.001). The rise of [Na+]i occurred over 1-2 min and showed only minimal recovery after 30 min. Confocal images of the distribution of [Na+]i showed a spatially uniform distribution both at rest and after eccentric contractions. Gd3+ (20 μM) had no effect on resting [Na+]i or control tetanic force but prevented the rise of [Na+]i and reduced the force deficit after eccentric damage. These data suggest that Na+ entry after eccentric contractions may occur principally through stretch-sensitive channels.
Persistent Identifierhttp://hdl.handle.net/10722/171715
ISSN
2015 Impact Factor: 3.004
2015 SCImago Journal Rankings: 1.488
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYeung, EWen_US
dc.contributor.authorBallard, HJen_US
dc.contributor.authorBourreau, JPen_US
dc.contributor.authorAllen, DGen_US
dc.date.accessioned2012-10-30T06:16:34Z-
dc.date.available2012-10-30T06:16:34Z-
dc.date.issued2003en_US
dc.identifier.citationJournal Of Applied Physiology, 2003, v. 94 n. 6, p. 2475-2482en_US
dc.identifier.issn8750-7587en_US
dc.identifier.urihttp://hdl.handle.net/10722/171715-
dc.description.abstractThe effect of eccentric contractions on intracellular Na+ concentration ([Na+]i) and its distribution were examined in isolated rat and mouse muscle fiber bundles. [Na+]i was measured with either Na+-binding benzofuran isophthalate or sodium green. Ten isometric contractions had no significant effect on force (measured after 5 min of recovery) and caused no significant change in the resting [Na+]i (7.2 ± 0.5 mM). In contrast 10 eccentric contractions (40% stretch at 4 muscle lengths/s) reduced developed force at 100 Hz to 45 ± 3% of control and increased [Na+]i to 16.3 ± 1.6 mM (n = 6; P < 0.001). The rise of [Na+]i occurred over 1-2 min and showed only minimal recovery after 30 min. Confocal images of the distribution of [Na+]i showed a spatially uniform distribution both at rest and after eccentric contractions. Gd3+ (20 μM) had no effect on resting [Na+]i or control tetanic force but prevented the rise of [Na+]i and reduced the force deficit after eccentric damage. These data suggest that Na+ entry after eccentric contractions may occur principally through stretch-sensitive channels.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Applied Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBenzofuransen_US
dc.subject.meshElectric Stimulationen_US
dc.subject.meshEnzyme Inhibitors - Pharmacologyen_US
dc.subject.meshEthers, Cyclicen_US
dc.subject.meshFluorescent Dyesen_US
dc.subject.meshGadolinium - Pharmacologyen_US
dc.subject.meshIntracellular Membranes - Metabolismen_US
dc.subject.meshIon Channels - Antagonists & Inhibitors - Metabolismen_US
dc.subject.meshIsometric Contraction - Drug Effects - Physiologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMicroscopy, Confocalen_US
dc.subject.meshMuscle Contraction - Drug Effects - Physiologyen_US
dc.subject.meshMuscle Fibers, Skeletal - Metabolismen_US
dc.subject.meshMuscle, Skeletal - Physiologyen_US
dc.subject.meshOrganic Chemicalsen_US
dc.subject.meshOsmolar Concentrationen_US
dc.subject.meshOuabain - Pharmacologyen_US
dc.subject.meshRatsen_US
dc.subject.meshSodium - Metabolismen_US
dc.subject.meshSodium-Potassium-Exchanging Atpase - Antagonists & Inhibitorsen_US
dc.subject.meshTissue Distributionen_US
dc.titleIntracellular sodium in mammalian muscle fibers after eccentric contractionsen_US
dc.typeArticleen_US
dc.identifier.emailBallard, HJ:ballard@hkucc.hku.hken_US
dc.identifier.emailBourreau, JP:bourreau@hkucc.hku.hken_US
dc.identifier.authorityBallard, HJ=rp00367en_US
dc.identifier.authorityBourreau, JP=rp00389en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1152/japplphysiol.01128.2002-
dc.identifier.pmid12588791-
dc.identifier.scopuseid_2-s2.0-0038516864en_US
dc.identifier.hkuros82589-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038516864&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume94en_US
dc.identifier.issue6en_US
dc.identifier.spage2475en_US
dc.identifier.epage2482en_US
dc.identifier.isiWOS:000182699800048-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYeung, EW=7101971748en_US
dc.identifier.scopusauthoridBallard, HJ=7005286310en_US
dc.identifier.scopusauthoridBourreau, JP=7003927886en_US
dc.identifier.scopusauthoridAllen, DG=35509336700en_US

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