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Article: Co-expression of adrenomedullin and adrenomedullin receptors in rat epididymis: Distinct physiological actions on anion transport

TitleCo-expression of adrenomedullin and adrenomedullin receptors in rat epididymis: Distinct physiological actions on anion transport
Authors
Issue Date2003
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
Biology Of Reproduction, 2003, v. 68 n. 6, p. 2005-2012 How to Cite?
AbstractAdrenomedullin (AM) has been found in the brain as well as in various peripheral tissues, including reproductive organs such as the testis and the prostate. Here, we report the expression of AM in the rat epididymis and its role in anion secretion. Whole-epididymal extracts had 35.3 ± 1.4 fmol of immunoreactive AM per mg of protein, and immunocytochemical studies showed positive AM immunostaining in the epithelial cells. By solution-hybridization-RNase protection assay, preproAM mRNA was detected at high levels in the epididymis. Gel filtration chromatography of AM showed two peaks, with the predominant one eluting at the position of authentic rat AM (1-50). Specific binding of AM to the epididymis, which could be displaced by calcitonin gene-related peptide, was observed. The epididymis also bound to calcitonin gene-related peptide, and this was displaceable by AM. Furthermore, the epididymis was shown to co-express mRNA encoding the calcitonin receptor-like receptor and receptor activity-modifying proteins, RAMP1/RAMP2. The corpus region had the highest AM level and gene expression and the lowest active peptide:precursor ratio. However, mRNA levels of the receptor and the receptor activity-modifying proteins were similar in all regions. In monolayer cultures derived from the rat epididymal cells, AM stimulated short-circuit current on the luminal side in a dose-dependent manner. Our results demonstrate the presence of AM, preproAM mRNA, AM receptors, and specific-binding sites in the rat epididymis as well as the possible role of AM in the regulation of electrolyte and fluid secretion in the epididymis.
Persistent Identifierhttp://hdl.handle.net/10722/171713
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHwang, ISSen_US
dc.contributor.authorAutelitano, DJen_US
dc.contributor.authorWong, PYDen_US
dc.contributor.authorLeung, GPHen_US
dc.contributor.authorTang, Fen_US
dc.date.accessioned2012-10-30T06:16:33Z-
dc.date.available2012-10-30T06:16:33Z-
dc.date.issued2003en_US
dc.identifier.citationBiology Of Reproduction, 2003, v. 68 n. 6, p. 2005-2012en_US
dc.identifier.issn0006-3363en_US
dc.identifier.urihttp://hdl.handle.net/10722/171713-
dc.description.abstractAdrenomedullin (AM) has been found in the brain as well as in various peripheral tissues, including reproductive organs such as the testis and the prostate. Here, we report the expression of AM in the rat epididymis and its role in anion secretion. Whole-epididymal extracts had 35.3 ± 1.4 fmol of immunoreactive AM per mg of protein, and immunocytochemical studies showed positive AM immunostaining in the epithelial cells. By solution-hybridization-RNase protection assay, preproAM mRNA was detected at high levels in the epididymis. Gel filtration chromatography of AM showed two peaks, with the predominant one eluting at the position of authentic rat AM (1-50). Specific binding of AM to the epididymis, which could be displaced by calcitonin gene-related peptide, was observed. The epididymis also bound to calcitonin gene-related peptide, and this was displaceable by AM. Furthermore, the epididymis was shown to co-express mRNA encoding the calcitonin receptor-like receptor and receptor activity-modifying proteins, RAMP1/RAMP2. The corpus region had the highest AM level and gene expression and the lowest active peptide:precursor ratio. However, mRNA levels of the receptor and the receptor activity-modifying proteins were similar in all regions. In monolayer cultures derived from the rat epididymal cells, AM stimulated short-circuit current on the luminal side in a dose-dependent manner. Our results demonstrate the presence of AM, preproAM mRNA, AM receptors, and specific-binding sites in the rat epididymis as well as the possible role of AM in the regulation of electrolyte and fluid secretion in the epididymis.en_US
dc.languageengen_US
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_US
dc.relation.ispartofBiology of Reproductionen_US
dc.subject.meshAdrenomedullinen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnion Transport Proteins - Physiologyen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshCalcitonin Gene-Related Peptide - Pharmacologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshChromatography, Gelen_US
dc.subject.meshEpididymis - Drug Effects - Metabolismen_US
dc.subject.meshIntracellular Signaling Peptides And Proteinsen_US
dc.subject.meshIodine Radioisotopes - Diagnostic Useen_US
dc.subject.meshKineticsen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Proteins - Biosynthesisen_US
dc.subject.meshNuclease Protection Assaysen_US
dc.subject.meshPeptides - Metabolismen_US
dc.subject.meshProtein Precursors - Biosynthesisen_US
dc.subject.meshRadioimmunoassayen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptor Activity-Modifying Protein 1en_US
dc.subject.meshReceptor Activity-Modifying Protein 2en_US
dc.subject.meshReceptor Activity-Modifying Proteinsen_US
dc.subject.meshReceptors, Adrenomedullinen_US
dc.subject.meshReceptors, Calcitonin Gene-Related Peptide - Metabolismen_US
dc.subject.meshReceptors, Peptide - Biosynthesisen_US
dc.titleCo-expression of adrenomedullin and adrenomedullin receptors in rat epididymis: Distinct physiological actions on anion transporten_US
dc.typeArticleen_US
dc.identifier.emailLeung, GPH:gphleung@hkucc.hku.hken_US
dc.identifier.emailTang, F:ftang@hkucc.hku.hken_US
dc.identifier.emailHwang, ISS: isabelbelbel@hotmail.com-
dc.identifier.authorityLeung, GPH=rp00234en_US
dc.identifier.authorityTang, F=rp00327en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1095/biolreprod.102.011015en_US
dc.identifier.pmid12606469-
dc.identifier.scopuseid_2-s2.0-0037837100en_US
dc.identifier.hkuros81393-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037837100&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume68en_US
dc.identifier.issue6en_US
dc.identifier.spage2005en_US
dc.identifier.epage2012en_US
dc.identifier.isiWOS:000183130000009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHwang, ISS=7201615103en_US
dc.identifier.scopusauthoridAutelitano, DJ=7003803451en_US
dc.identifier.scopusauthoridWong, PYD=7403980262en_US
dc.identifier.scopusauthoridLeung, GPH=35963668200en_US
dc.identifier.scopusauthoridTang, F=7201979770en_US

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