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Article: Variations of mt1 melatonin receptor density in the rat uterus during decidualization, the estrous cycle and in response to exogenous steroid treatment

TitleVariations of mt1 melatonin receptor density in the rat uterus during decidualization, the estrous cycle and in response to exogenous steroid treatment
Authors
Issue Date2002
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPI
Citation
Journal Of Pineal Research, 2002, v. 33 n. 3, p. 140-145 How to Cite?
AbstractThe expression of mt1 receptor protein in the rat uterus was investigated using an anti-mt1 polyclonal antibody against the rat mt1 receptor. A melatonin receptor protein of 37 kDa was detectable by Western blotting in the rat uterine membrane preparations. Autoradiography with the melatonin ligand, 2-[125I]iodomelatonin, was used to localize melatonin receptors in the uterus of the estrous rats and to study the changes of melatonin receptors in pregnancy. Melatonin receptors were found to be localized in the estrous rat uterine antimesometrial stroma. As decidualization of the uterine stroma progressed during pregnancy, the melatonin binding sites were progressively reduced and became confined to the antimesometrial non-decidualized outer stroma. 2-[125I]Iodomelatonin binding sites were not seen in the mesometrial stromal cells during pregnancy. The role of ovarian hormones in the regulation of uterine melatonin receptors was examined by studying the binding at various phases of the estrous cycle, after ovariectomy with and without follow-on treatment of estradiol (E2), progesterone (P4) or both. 2-[125I]Iodomelatonin binding in the rat uterus fluctuated during the estrous cycle, being lowest during metestrus. Ovariectomy caused an almost 70% reduction of 2-[125I]Iodomelatonin binding compared with the control. Injections of ovariectomized (OVX) rats with E2 or P4 alone or in combination for 11 days induced a partial restoration of 2-[125I]iodomelatonin binding in the OVX rats. The results show that mt1 melatonin receptors in the rat antimesometrial stroma are regulated by ovarian hormones.
Persistent Identifierhttp://hdl.handle.net/10722/171706
ISSN
2015 Impact Factor: 9.314
2015 SCImago Journal Rankings: 2.655
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhao, Hen_US
dc.contributor.authorPang, SFen_US
dc.contributor.authorPoon, AMSen_US
dc.date.accessioned2012-10-30T06:16:31Z-
dc.date.available2012-10-30T06:16:31Z-
dc.date.issued2002en_US
dc.identifier.citationJournal Of Pineal Research, 2002, v. 33 n. 3, p. 140-145en_US
dc.identifier.issn0742-3098en_US
dc.identifier.urihttp://hdl.handle.net/10722/171706-
dc.description.abstractThe expression of mt1 receptor protein in the rat uterus was investigated using an anti-mt1 polyclonal antibody against the rat mt1 receptor. A melatonin receptor protein of 37 kDa was detectable by Western blotting in the rat uterine membrane preparations. Autoradiography with the melatonin ligand, 2-[125I]iodomelatonin, was used to localize melatonin receptors in the uterus of the estrous rats and to study the changes of melatonin receptors in pregnancy. Melatonin receptors were found to be localized in the estrous rat uterine antimesometrial stroma. As decidualization of the uterine stroma progressed during pregnancy, the melatonin binding sites were progressively reduced and became confined to the antimesometrial non-decidualized outer stroma. 2-[125I]Iodomelatonin binding sites were not seen in the mesometrial stromal cells during pregnancy. The role of ovarian hormones in the regulation of uterine melatonin receptors was examined by studying the binding at various phases of the estrous cycle, after ovariectomy with and without follow-on treatment of estradiol (E2), progesterone (P4) or both. 2-[125I]Iodomelatonin binding in the rat uterus fluctuated during the estrous cycle, being lowest during metestrus. Ovariectomy caused an almost 70% reduction of 2-[125I]Iodomelatonin binding compared with the control. Injections of ovariectomized (OVX) rats with E2 or P4 alone or in combination for 11 days induced a partial restoration of 2-[125I]iodomelatonin binding in the OVX rats. The results show that mt1 melatonin receptors in the rat antimesometrial stroma are regulated by ovarian hormones.en_US
dc.languageengen_US
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPIen_US
dc.relation.ispartofJournal of Pineal Researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDecidua - Drug Effects - Metabolismen_US
dc.subject.meshEstradiol - Pharmacologyen_US
dc.subject.meshEstrus - Drug Effects - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshMelatonin - Analogs & Derivatives - Metabolismen_US
dc.subject.meshOvariectomyen_US
dc.subject.meshPregnancyen_US
dc.subject.meshProgesterone - Pharmacologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptors, Cell Surface - Drug Effects - Metabolismen_US
dc.subject.meshReceptors, Cytoplasmic And Nuclear - Drug Effects - Metabolismen_US
dc.subject.meshReceptors, Melatoninen_US
dc.subject.meshUterus - Drug Effects - Metabolismen_US
dc.titleVariations of mt1 melatonin receptor density in the rat uterus during decidualization, the estrous cycle and in response to exogenous steroid treatmenten_US
dc.typeArticleen_US
dc.identifier.emailPoon, AMS:amspoon@hkucc.hku.hken_US
dc.identifier.authorityPoon, AMS=rp00354en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1034/j.1600-079X.2002.02898.xen_US
dc.identifier.pmid12220327-
dc.identifier.scopuseid_2-s2.0-0036771820en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036771820&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume33en_US
dc.identifier.issue3en_US
dc.identifier.spage140en_US
dc.identifier.epage145en_US
dc.identifier.isiWOS:000177852400003-
dc.publisher.placeDenmarken_US
dc.identifier.scopusauthoridZhao, H=14821083400en_US
dc.identifier.scopusauthoridPang, SF=7402528719en_US
dc.identifier.scopusauthoridPoon, AMS=7103068868en_US

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