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Article: Attenuated "cross talk" between κ-opioid receptors and β-adrenoceptors in the heart of chronically hypoxic rats
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TitleAttenuated "cross talk" between κ-opioid receptors and β-adrenoceptors in the heart of chronically hypoxic rats
 
AuthorsShan, J1
Yu, XC1
Fung, ML1
Wong, TM1
 
Issue Date2002
 
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00424/index.htm
 
CitationPflugers Archiv European Journal Of Physiology, 2002, v. 444 n. 1-2, p. 126-132 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00424-002-0814-0
 
AbstractAt 0.1-1 μM, U50488H, a κ-opioid receptor agonist, inhibited the stimulatory effects of 1 μM isoprenaline, a β-adrenoceptor agonist, on the electrically induced intracellular Ca2+ ([Ca2+]i) transient and cAMP accumulation ("cross talk" between κ-opioid receptors and β-adrenoceptors) in the presence of 1 μM ICI118,551, a β2-adrenoceptor antagonist in ventricular myocytes from both normoxic and chronically hypoxic rats. Pretreatment with 20 μg/ml cholera toxin increased the ADP-ribosylation of Gsα subunits and the electrically induced [Ca2+]i transient in both normoxic and chronically hypoxic rats. The effects of cholera toxin were inhibited by 1 μM U50488H and the inhibitory effect of U50488H was attenuated in chronically hypoxic rats. Similarly, 1 μM forskolin also increased the electrically induced [Ca2+]i transient and cAMP accumulation, which were both inhibited by U50488H, in both normoxic and chronically hypoxic rats. The inhibitory effects of 1 μM U50488H were significantly attenuated in chronically hypoxic rats. The results are novel findings, in that the "cross talk" between κ-opioid receptors and β-adrenoceptors is attenuated following chronic hypoxia. The attenuation may be due to decreased responses of Gs-protein and adenylyl cyclase to κ-opioid receptor activation in addition to desensitization of the receptor itself.
 
ISSN0031-6768
2013 Impact Factor: 3.073
 
DOIhttp://dx.doi.org/10.1007/s00424-002-0814-0
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorShan, J
 
dc.contributor.authorYu, XC
 
dc.contributor.authorFung, ML
 
dc.contributor.authorWong, TM
 
dc.date.accessioned2012-10-30T06:16:30Z
 
dc.date.available2012-10-30T06:16:30Z
 
dc.date.issued2002
 
dc.description.abstractAt 0.1-1 μM, U50488H, a κ-opioid receptor agonist, inhibited the stimulatory effects of 1 μM isoprenaline, a β-adrenoceptor agonist, on the electrically induced intracellular Ca2+ ([Ca2+]i) transient and cAMP accumulation ("cross talk" between κ-opioid receptors and β-adrenoceptors) in the presence of 1 μM ICI118,551, a β2-adrenoceptor antagonist in ventricular myocytes from both normoxic and chronically hypoxic rats. Pretreatment with 20 μg/ml cholera toxin increased the ADP-ribosylation of Gsα subunits and the electrically induced [Ca2+]i transient in both normoxic and chronically hypoxic rats. The effects of cholera toxin were inhibited by 1 μM U50488H and the inhibitory effect of U50488H was attenuated in chronically hypoxic rats. Similarly, 1 μM forskolin also increased the electrically induced [Ca2+]i transient and cAMP accumulation, which were both inhibited by U50488H, in both normoxic and chronically hypoxic rats. The inhibitory effects of 1 μM U50488H were significantly attenuated in chronically hypoxic rats. The results are novel findings, in that the "cross talk" between κ-opioid receptors and β-adrenoceptors is attenuated following chronic hypoxia. The attenuation may be due to decreased responses of Gs-protein and adenylyl cyclase to κ-opioid receptor activation in addition to desensitization of the receptor itself.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationPflugers Archiv European Journal Of Physiology, 2002, v. 444 n. 1-2, p. 126-132 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00424-002-0814-0
 
dc.identifier.doihttp://dx.doi.org/10.1007/s00424-002-0814-0
 
dc.identifier.epage132
 
dc.identifier.hkuros71971
 
dc.identifier.issn0031-6768
2013 Impact Factor: 3.073
 
dc.identifier.issue1-2
 
dc.identifier.pmid11976924
 
dc.identifier.scopuseid_2-s2.0-0036261285
 
dc.identifier.spage126
 
dc.identifier.urihttp://hdl.handle.net/10722/171705
 
dc.identifier.volume444
 
dc.languageeng
 
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00424/index.htm
 
dc.publisher.placeGermany
 
dc.relation.ispartofPflugers Archiv European Journal of Physiology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdenosine Diphosphate Ribose - Metabolism
 
dc.subject.meshAdenylate Cyclase - Metabolism
 
dc.subject.meshAdrenergic Beta-Agonists - Pharmacology
 
dc.subject.meshAnimals
 
dc.subject.meshAnoxia - Physiopathology
 
dc.subject.meshCalcium Signaling - Drug Effects
 
dc.subject.meshCardiomegaly - Physiopathology
 
dc.subject.meshCholera Toxin - Pharmacology
 
dc.subject.meshChronic Disease
 
dc.subject.meshCyclic Amp - Metabolism
 
dc.subject.meshDose-Response Relationship, Drug
 
dc.subject.meshFluorescent Dyes
 
dc.subject.meshForskolin - Pharmacology
 
dc.subject.meshFura-2
 
dc.subject.meshGtp-Binding Protein Alpha Subunits, Gs - Metabolism
 
dc.subject.meshHeart - Physiopathology
 
dc.subject.meshIsoproterenol - Pharmacology
 
dc.subject.meshMale
 
dc.subject.meshRats
 
dc.subject.meshRats, Sprague-Dawley
 
dc.subject.meshReceptor Cross-Talk - Physiology
 
dc.subject.meshReceptors, Adrenergic, Beta - Physiology
 
dc.subject.meshReceptors, Opioid, Kappa - Physiology
 
dc.titleAttenuated "cross talk" between κ-opioid receptors and β-adrenoceptors in the heart of chronically hypoxic rats
 
dc.typeArticle
 
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<subject.mesh>Adenosine Diphosphate Ribose - Metabolism</subject.mesh>
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Author Affiliations
  1. The University of Hong Kong