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Article: Effect of eccentric contraction-induced injury on force and intracellular pH in rat skeletal muscles

TitleEffect of eccentric contraction-induced injury on force and intracellular pH in rat skeletal muscles
Authors
Issue Date2002
Citation
Journal Of Applied Physiology, 2002, v. 92 n. 1, p. 93-99 How to Cite?
AbstractThe effect of eccentric contraction on force generation and intracellular pH (pHi) regulation was investigated in rat soleus muscle. Eccentric muscle damage was induced by stretching muscle bundles by 30% of the optimal length for a series of 10 tetani. After eccentric contractions, there was reduction in force at all stimulation frequencies and a greater reduction in relative force at low-stimulus frequencies. There was also a shift of optimal length to longer lengths. pHi was measured with a pH-sensitive probe, 2′,7′-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein AM. pHi regulation was studied by inducing an acute acid load with the removal of 20-40 mM ammonium chloride, and the rate of pHi recovery was monitored. The acid extrusion rate was obtained by multiplying the rate of pHi recovery by the buffering power. The resting pHi after eccentric contractions was more acidic, and the rate of recovery from acid load post-eccentric contractions was slower than that from postisometric controls. This is further supported by the slower acid extrusion rate. Amiloride slowed the recovery from an acid load in control experiments. Because the Na+/H+ exchanger is the dominant mechanism for the recovery of pHi, this suggests that the impairment in the ability of the muscle to regulate pHi after eccentric contractions is caused by decreased activity of the Na+/H+ exchanger.
Persistent Identifierhttp://hdl.handle.net/10722/171703
ISSN
2015 Impact Factor: 3.004
2015 SCImago Journal Rankings: 1.488
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYeung, EWen_US
dc.contributor.authorBourreau, JPen_US
dc.contributor.authorAllenand, DGen_US
dc.contributor.authorBallard, HJen_US
dc.date.accessioned2012-10-30T06:16:30Z-
dc.date.available2012-10-30T06:16:30Z-
dc.date.issued2002en_US
dc.identifier.citationJournal Of Applied Physiology, 2002, v. 92 n. 1, p. 93-99en_US
dc.identifier.issn8750-7587en_US
dc.identifier.urihttp://hdl.handle.net/10722/171703-
dc.description.abstractThe effect of eccentric contraction on force generation and intracellular pH (pHi) regulation was investigated in rat soleus muscle. Eccentric muscle damage was induced by stretching muscle bundles by 30% of the optimal length for a series of 10 tetani. After eccentric contractions, there was reduction in force at all stimulation frequencies and a greater reduction in relative force at low-stimulus frequencies. There was also a shift of optimal length to longer lengths. pHi was measured with a pH-sensitive probe, 2′,7′-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein AM. pHi regulation was studied by inducing an acute acid load with the removal of 20-40 mM ammonium chloride, and the rate of pHi recovery was monitored. The acid extrusion rate was obtained by multiplying the rate of pHi recovery by the buffering power. The resting pHi after eccentric contractions was more acidic, and the rate of recovery from acid load post-eccentric contractions was slower than that from postisometric controls. This is further supported by the slower acid extrusion rate. Amiloride slowed the recovery from an acid load in control experiments. Because the Na+/H+ exchanger is the dominant mechanism for the recovery of pHi, this suggests that the impairment in the ability of the muscle to regulate pHi after eccentric contractions is caused by decreased activity of the Na+/H+ exchanger.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Applied Physiologyen_US
dc.subject.meshAlgorithmsen_US
dc.subject.meshAmiloride - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDiuretics - Pharmacologyen_US
dc.subject.meshElectric Stimulationen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscle Contraction - Physiologyen_US
dc.subject.meshMuscle Fibers, Skeletal - Physiologyen_US
dc.subject.meshMuscle, Skeletal - Injuries - Physiopathologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.titleEffect of eccentric contraction-induced injury on force and intracellular pH in rat skeletal musclesen_US
dc.typeArticleen_US
dc.identifier.emailBourreau, JP:bourreau@hkucc.hku.hken_US
dc.identifier.emailBallard, HJ:ballard@hkucc.hku.hken_US
dc.identifier.authorityBourreau, JP=rp00389en_US
dc.identifier.authorityBallard, HJ=rp00367en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid11744647-
dc.identifier.scopuseid_2-s2.0-0036092944en_US
dc.identifier.hkuros72946-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036092944&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume92en_US
dc.identifier.issue1en_US
dc.identifier.spage93en_US
dc.identifier.epage99en_US
dc.identifier.isiWOS:000173205400013-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYeung, EW=7101971748en_US
dc.identifier.scopusauthoridBourreau, JP=7003927886en_US
dc.identifier.scopusauthoridAllenand, DG=19033546400en_US
dc.identifier.scopusauthoridBallard, HJ=7005286310en_US

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