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Article: Functional visualization of the separate but interacting calcium stores sensitive to NAADP and cyclic ADP-ribose

TitleFunctional visualization of the separate but interacting calcium stores sensitive to NAADP and cyclic ADP-ribose
Authors
Issue Date2000
Citation
Journal Of Cell Science, 2000, v. 113 n. 24, p. 4413-4420 How to Cite?
AbstractCells possess multiple Ca2+ stores and their selective mobilization provides the spatial-temporal Ca2+ signals crucial in regulating diverse cellular functions. Except for the inositol trisphosphate (IP3)-sensitive Ca2+ stores, the identities and the mechanisms of how these internal stores are mobilized are largely unknown. In this study, we describe two Ca2+ stores, one of which is regulated by cyclic ADP-ribose (cADPR) and the other by nicotinic acid adenine dinucleotide phosphate (NAADP). We took advantage of the large size of the sea urchin egg and stratified its organelles by centrifugation. Using photolysis to produce either uniform or localized increases of cADPR and NAADP from their respective caged analogs, the two separate stores could be visually identified by Ca2+ imaging and shown to be segregated to the opposite poles of the eggs. The cADPR-pole also contained the IP3-sensitive Ca2+ stores, the egg nucleus and the endoplasmic reticulum (ER); the latter was visualized using Bodipy-thapsigargin. On the other hand, the mitochondria, as visualized by rhodamine 123, were segregated to the opposite pole together with the NAADP-sensitive calcium stores. Fertilization of the stratified eggs elicited a Ca2+ wave starting at the cADPR-pole and propagating toward the NAADP-pole. These results provide the first direct and visual evidence that the NAADP-sensitive Ca2+ stores are novel and distinct from the ER. During fertilization, communicating signals appear to be transmitted from the ER to NAADP-sensitive Ca2+ stores, leading to their activation.
Persistent Identifierhttp://hdl.handle.net/10722/171681
ISSN
2015 Impact Factor: 4.706
2015 SCImago Journal Rankings: 3.501
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, HCen_US
dc.contributor.authorAarhus, Ren_US
dc.date.accessioned2012-10-30T06:16:19Z-
dc.date.available2012-10-30T06:16:19Z-
dc.date.issued2000en_US
dc.identifier.citationJournal Of Cell Science, 2000, v. 113 n. 24, p. 4413-4420en_US
dc.identifier.issn0021-9533en_US
dc.identifier.urihttp://hdl.handle.net/10722/171681-
dc.description.abstractCells possess multiple Ca2+ stores and their selective mobilization provides the spatial-temporal Ca2+ signals crucial in regulating diverse cellular functions. Except for the inositol trisphosphate (IP3)-sensitive Ca2+ stores, the identities and the mechanisms of how these internal stores are mobilized are largely unknown. In this study, we describe two Ca2+ stores, one of which is regulated by cyclic ADP-ribose (cADPR) and the other by nicotinic acid adenine dinucleotide phosphate (NAADP). We took advantage of the large size of the sea urchin egg and stratified its organelles by centrifugation. Using photolysis to produce either uniform or localized increases of cADPR and NAADP from their respective caged analogs, the two separate stores could be visually identified by Ca2+ imaging and shown to be segregated to the opposite poles of the eggs. The cADPR-pole also contained the IP3-sensitive Ca2+ stores, the egg nucleus and the endoplasmic reticulum (ER); the latter was visualized using Bodipy-thapsigargin. On the other hand, the mitochondria, as visualized by rhodamine 123, were segregated to the opposite pole together with the NAADP-sensitive calcium stores. Fertilization of the stratified eggs elicited a Ca2+ wave starting at the cADPR-pole and propagating toward the NAADP-pole. These results provide the first direct and visual evidence that the NAADP-sensitive Ca2+ stores are novel and distinct from the ER. During fertilization, communicating signals appear to be transmitted from the ER to NAADP-sensitive Ca2+ stores, leading to their activation.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Cell Scienceen_US
dc.subject.meshAdenosine Diphosphate Ribose - Analogs & Derivatives - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCalcium - Metabolismen_US
dc.subject.meshCalcium Signaling - Physiologyen_US
dc.subject.meshCalcium-Transporting Atpases - Metabolismen_US
dc.subject.meshCyclic Adp-Riboseen_US
dc.subject.meshNadp - Analogs & Derivatives - Metabolismen_US
dc.subject.meshSea Urchinsen_US
dc.titleFunctional visualization of the separate but interacting calcium stores sensitive to NAADP and cyclic ADP-riboseen_US
dc.typeArticleen_US
dc.identifier.emailLee, HC:leehc@hku.hken_US
dc.identifier.authorityLee, HC=rp00545en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid11082034-
dc.identifier.scopuseid_2-s2.0-0034496224en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034496224&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume113en_US
dc.identifier.issue24en_US
dc.identifier.spage4413en_US
dc.identifier.epage4420en_US
dc.identifier.isiWOS:000166325600005-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLee, HC=26642959100en_US
dc.identifier.scopusauthoridAarhus, R=6701339421en_US

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