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Article: Modulation of CD157 expression in multi-lineage myeloid differentiation of promyelocytic cell lines

TitleModulation of CD157 expression in multi-lineage myeloid differentiation of promyelocytic cell lines
Authors
Issue Date2000
PublisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/ejcb
Citation
European Journal Of Cell Biology, 2000, v. 79 n. 10, p. 697-706 How to Cite?
AbstractCD157/BST-1 is expressed on mature myeloid cells but not on their precursors in vivo. Also CD38, a homologous gene to CD157, is upregulated in promyelocytic HL-60 cells by the monocyte and granulocyte differentiation-inducing 1α,25-dihydroxyvitamin D3 (VD3) and all-trans retinoic acid (ATRA), respectively. We have examined whether CD157 expression is upregulated when the promyeloid HL-60 and/or U937 cells are induced to differentiate into mature phenotypes in vitro. VD3 treatment irreversibly upregulated the expression of CD157 in HL-60 cells but not in U937 cells in a time- and concentration-dependent manner when analyzed by flow cytometry, immunoblotting and/or RT-PCR. Different monocyte and granulocyte lineage inducers induced CD157 expression to varying extents while the macrophage differentiation-inducing phorbol 12-myristate 13-acetate (PMA) induced its down-regulation. Time-kinetics of VD3 treatment of HL-60 cells showed that the appearance of CD157 and CD11b (a differentiation marker) antigens were not substantial up to 24 hours but increased subsequently although the appearance of CD38 became significant within 6 hours. Two-color staining of VD3-treated HL-60 cells displayed an apparently linear correlation between CD157 and CD11b expression. Dibutyryl cAMP (cAMP agonist) and forskolin (cAMP-increasing agent) augmented the VD3-dependent induction of CD157 and CD11b expression while PGE1 (cAMP-decreasing agent) inhibited it, suggesting the involvement of a cAMP-dependent mechanism in VD3-induced CD157 upregulation. Co-treatment of HL-60 cells with VD3 plus TNF-α or ara-C produced an additive effect on CD157 upregulation. The upregulated CD157 in the VD3-differentiated HL-60 cells was able to activate CD157-dependent tyrosine kinase signal when cross-linked with anti-CD157 antibody.
Persistent Identifierhttp://hdl.handle.net/10722/171676
ISSN
2015 Impact Factor: 4.011
2015 SCImago Journal Rankings: 2.075
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHussain, AMMen_US
dc.contributor.authorLee, HCen_US
dc.contributor.authorChang, CFen_US
dc.date.accessioned2012-10-30T06:16:18Z-
dc.date.available2012-10-30T06:16:18Z-
dc.date.issued2000en_US
dc.identifier.citationEuropean Journal Of Cell Biology, 2000, v. 79 n. 10, p. 697-706en_US
dc.identifier.issn0171-9335en_US
dc.identifier.urihttp://hdl.handle.net/10722/171676-
dc.description.abstractCD157/BST-1 is expressed on mature myeloid cells but not on their precursors in vivo. Also CD38, a homologous gene to CD157, is upregulated in promyelocytic HL-60 cells by the monocyte and granulocyte differentiation-inducing 1α,25-dihydroxyvitamin D3 (VD3) and all-trans retinoic acid (ATRA), respectively. We have examined whether CD157 expression is upregulated when the promyeloid HL-60 and/or U937 cells are induced to differentiate into mature phenotypes in vitro. VD3 treatment irreversibly upregulated the expression of CD157 in HL-60 cells but not in U937 cells in a time- and concentration-dependent manner when analyzed by flow cytometry, immunoblotting and/or RT-PCR. Different monocyte and granulocyte lineage inducers induced CD157 expression to varying extents while the macrophage differentiation-inducing phorbol 12-myristate 13-acetate (PMA) induced its down-regulation. Time-kinetics of VD3 treatment of HL-60 cells showed that the appearance of CD157 and CD11b (a differentiation marker) antigens were not substantial up to 24 hours but increased subsequently although the appearance of CD38 became significant within 6 hours. Two-color staining of VD3-treated HL-60 cells displayed an apparently linear correlation between CD157 and CD11b expression. Dibutyryl cAMP (cAMP agonist) and forskolin (cAMP-increasing agent) augmented the VD3-dependent induction of CD157 and CD11b expression while PGE1 (cAMP-decreasing agent) inhibited it, suggesting the involvement of a cAMP-dependent mechanism in VD3-induced CD157 upregulation. Co-treatment of HL-60 cells with VD3 plus TNF-α or ara-C produced an additive effect on CD157 upregulation. The upregulated CD157 in the VD3-differentiated HL-60 cells was able to activate CD157-dependent tyrosine kinase signal when cross-linked with anti-CD157 antibody.en_US
dc.languageengen_US
dc.publisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/ejcben_US
dc.relation.ispartofEuropean Journal of Cell Biologyen_US
dc.subject.meshAdp-Ribosyl Cyclaseen_US
dc.subject.meshAntigens, Cden_US
dc.subject.meshBucladesine - Metabolismen_US
dc.subject.meshCalcitriol - Metabolismen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Lineageen_US
dc.subject.meshCell Separationen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDetergents - Pharmacologyen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshDown-Regulationen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshEnzyme Activationen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshForskolin - Metabolismen_US
dc.subject.meshGpi-Linked Proteinsen_US
dc.subject.meshGranulocytes - Cytology - Metabolismen_US
dc.subject.meshHl-60 Cellsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshKineticsen_US
dc.subject.meshMacrophage-1 Antigen - Biosynthesisen_US
dc.subject.meshMembrane Glycoproteins - Biosynthesisen_US
dc.subject.meshMonocytes - Metabolismen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshPhosphorylationen_US
dc.subject.meshPolyethylene Glycols - Pharmacologyen_US
dc.subject.meshPrecipitin Testsen_US
dc.subject.meshProtein-Tyrosine Kinases - Metabolismen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshTetradecanoylphorbol Acetate - Metabolismen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTyrosine - Metabolismen_US
dc.subject.meshU937 Cellsen_US
dc.subject.meshUp-Regulationen_US
dc.titleModulation of CD157 expression in multi-lineage myeloid differentiation of promyelocytic cell linesen_US
dc.typeArticleen_US
dc.identifier.emailLee, HC:leehc@hku.hken_US
dc.identifier.authorityLee, HC=rp00545en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1078/0171-9335-00099-
dc.identifier.pmid11089918-
dc.identifier.scopuseid_2-s2.0-0033753933en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033753933&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume79en_US
dc.identifier.issue10en_US
dc.identifier.spage697en_US
dc.identifier.epage706en_US
dc.identifier.isiWOS:000165291700005-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridHussain, AMM=9733025800en_US
dc.identifier.scopusauthoridLee, HC=26642959100en_US
dc.identifier.scopusauthoridChang, CF=7407035747en_US

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