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Article: Co-expression of prepro-adrenomedullin with a putative adrenomedullin receptor gene in vascular smooth muscle

TitleCo-expression of prepro-adrenomedullin with a putative adrenomedullin receptor gene in vascular smooth muscle
Authors
Issue Date1999
PublisherPortland Press Ltd. The Journal's web site is located at http://www.clinsci.org/
Citation
Clinical Science, 1999, v. 96 n. 5, p. 493-498 How to Cite?
AbstractThe prepro-adrenomedullin (prepro-AM) gene encodes several biologically active peptides, including the potent vasodilator AM. At least part of the vasodilator action of AM appears to be mediated via interactions with receptors on vascular smooth muscle cells (VSMC); however, the specific receptors involved are not known. The aim of the present study was to identify putative AM receptor genes that are co-expressed with AM in cultured rat aortic VSMC that may mediate the actions of AM. AM mRNA was shown to be expressed in rat aortic VSMC cultures. In acute (4 h) secretion studies, only 20% of the total immunoreactive AM was intracellular, with the majority (80%) found in the medium, indicating active release of AM peptide from VSMC. Using highly specific ribonuclease protection analysis, mRNAs encoding three putative AM receptors [LI, calcitonin-receptor-like receptor (CRLR) and RDC1] were shown to be present at high concentrations in RNA extracts from lung. In cultured VSMC, however, whereas RDC1 mRNA was expressed at relatively high concentrations, transcripts encoding CRLR and LI were not detected. The co-expression of prepro-AM mRNA with the RDC1 receptor implies that AM may act in a localized manner via this receptor to modulate VSMC function.
Persistent Identifierhttp://hdl.handle.net/10722/171668
ISSN
2015 Impact Factor: 4.996
2015 SCImago Journal Rankings: 2.427
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorAutelitano, DJen_US
dc.contributor.authorTang, Fen_US
dc.date.accessioned2012-10-30T06:16:15Z-
dc.date.available2012-10-30T06:16:15Z-
dc.date.issued1999en_US
dc.identifier.citationClinical Science, 1999, v. 96 n. 5, p. 493-498en_US
dc.identifier.issn0143-5221en_US
dc.identifier.urihttp://hdl.handle.net/10722/171668-
dc.description.abstractThe prepro-adrenomedullin (prepro-AM) gene encodes several biologically active peptides, including the potent vasodilator AM. At least part of the vasodilator action of AM appears to be mediated via interactions with receptors on vascular smooth muscle cells (VSMC); however, the specific receptors involved are not known. The aim of the present study was to identify putative AM receptor genes that are co-expressed with AM in cultured rat aortic VSMC that may mediate the actions of AM. AM mRNA was shown to be expressed in rat aortic VSMC cultures. In acute (4 h) secretion studies, only 20% of the total immunoreactive AM was intracellular, with the majority (80%) found in the medium, indicating active release of AM peptide from VSMC. Using highly specific ribonuclease protection analysis, mRNAs encoding three putative AM receptors [LI, calcitonin-receptor-like receptor (CRLR) and RDC1] were shown to be present at high concentrations in RNA extracts from lung. In cultured VSMC, however, whereas RDC1 mRNA was expressed at relatively high concentrations, transcripts encoding CRLR and LI were not detected. The co-expression of prepro-AM mRNA with the RDC1 receptor implies that AM may act in a localized manner via this receptor to modulate VSMC function.en_US
dc.languageengen_US
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.clinsci.org/en_US
dc.relation.ispartofClinical Scienceen_US
dc.subject.meshAdrenomedullinen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAortaen_US
dc.subject.meshCalcitonin Receptor-Like Proteinen_US
dc.subject.meshCulture Techniquesen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Proteins - Geneticsen_US
dc.subject.meshMuscle, Smooth, Vascular - Chemistryen_US
dc.subject.meshPeptides - Analysisen_US
dc.subject.meshProtein Precursors - Genetics - Metabolismen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Wkyen_US
dc.subject.meshReceptors, Adrenomedullinen_US
dc.subject.meshReceptors, Calcitonin - Geneticsen_US
dc.subject.meshReceptors, Calcitonin Gene-Related Peptide - Geneticsen_US
dc.subject.meshReceptors, Peptideen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.titleCo-expression of prepro-adrenomedullin with a putative adrenomedullin receptor gene in vascular smooth muscleen_US
dc.typeArticleen_US
dc.identifier.emailTang, F:ftang@hkucc.hku.hken_US
dc.identifier.authorityTang, F=rp00327en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1042/CS19980311en_US
dc.identifier.pmid10209081-
dc.identifier.scopuseid_2-s2.0-0032914069en_US
dc.identifier.hkuros53225-
dc.identifier.hkuros44386-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032914069&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume96en_US
dc.identifier.issue5en_US
dc.identifier.spage493en_US
dc.identifier.epage498en_US
dc.identifier.isiWOS:000080341200008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridAutelitano, DJ=7003803451en_US
dc.identifier.scopusauthoridTang, F=7201979770en_US

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