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- Publisher Website: 10.1016/S0008-6363(98)00176-X
- Scopus: eid_2-s2.0-0032168269
- PMID: 9861308
- WOS: WOS:000075916700017
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Article: Impaired paracrine effect of endothelin-1 on vascular smooth muscle in streptozotocin-diabetic: Rats
Title | Impaired paracrine effect of endothelin-1 on vascular smooth muscle in streptozotocin-diabetic: Rats |
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Authors | |
Keywords | Diabetes Endothelin Paracrine Rat Receptors Vascular smooth muscle |
Issue Date | 1998 |
Publisher | Oxford University Press. The Journal's web site is located at http://cardiovascres.oxfordjournals.org |
Citation | Cardiovascular Research, 1998, v. 39 n. 3, p. 651-656 How to Cite? |
Abstract | Objective: This study was to examine the effect of streptozotocin- induced diabetes on endothelin-1 and its receptors in the mesenteric artery and in the thoracic aorta. Methods: Diabetes was induced in SD rats by streptozotocin. Insulin was given subcutaneously. Endothelin-1 levels in the plasma, thoracic aorta and mesenteric artery were measured using radioimmunoassay. The B(max) and K(d) values of endothelin-1 receptors in the mesenteric artery and in the thoracic aorta were analyzed using Scatchard plot analysis. Preproendothelin mRNA levels were examined using RT-PCR. Results: Endothelin-1 levels in the mesenteric artery (83.6±6.9 pg/mg protein) and in the thoracic aorta (73.9±8.2 pg/mg protein) increased in 2 week diabetic rats compared with both control (51.8±5.3, 46.3±5.9 pg/mg protein) and insulin treated rats (65.6±8.1, 48.1±4.2 pg/mg protein) but not in 4 week diabetic rats. There was no change in plasma endothelin-1 levels in these diabetic rats. The RT-PCR results indicated that preproendothelin mRNA levels in the mesenteric artery (0.38±0.02 vs 0.52±0.05 units) and in the thoracic aorta (0.45±0.06 vs 0.62±0.03 units) decreased in 4 week diabetic rats but not in 2 week diabetic rats. A significant increase in K(d) and B(max) of endothelin receptors in the mesenteric artery and in the thoracic aorta was observed in both 2 week (about 70%) and 4 week (80-85%) diabetic rats. Insulin replacement reversed the effects of diabetes on endothelin-1 peptide contents, preproendothelin mRNA levels and the binding activity in the blood vessels. Conclusion: Increased endothelin peptide content with no change in mRNA or decreased mRNA levels with no change in peptide content together with increased receptor binding sites and affinities might imply a decrease in endothelin release and therefore an impaired paracrine effect of endothelin on vascular smooth muscles in these STZ-diabetic rats. |
Persistent Identifier | http://hdl.handle.net/10722/171650 |
ISSN | 2023 Impact Factor: 10.2 2023 SCImago Journal Rankings: 2.809 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Wu, SQ | en_US |
dc.contributor.author | Tang, F | en_US |
dc.date.accessioned | 2012-10-30T06:16:09Z | - |
dc.date.available | 2012-10-30T06:16:09Z | - |
dc.date.issued | 1998 | en_US |
dc.identifier.citation | Cardiovascular Research, 1998, v. 39 n. 3, p. 651-656 | en_US |
dc.identifier.issn | 0008-6363 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171650 | - |
dc.description.abstract | Objective: This study was to examine the effect of streptozotocin- induced diabetes on endothelin-1 and its receptors in the mesenteric artery and in the thoracic aorta. Methods: Diabetes was induced in SD rats by streptozotocin. Insulin was given subcutaneously. Endothelin-1 levels in the plasma, thoracic aorta and mesenteric artery were measured using radioimmunoassay. The B(max) and K(d) values of endothelin-1 receptors in the mesenteric artery and in the thoracic aorta were analyzed using Scatchard plot analysis. Preproendothelin mRNA levels were examined using RT-PCR. Results: Endothelin-1 levels in the mesenteric artery (83.6±6.9 pg/mg protein) and in the thoracic aorta (73.9±8.2 pg/mg protein) increased in 2 week diabetic rats compared with both control (51.8±5.3, 46.3±5.9 pg/mg protein) and insulin treated rats (65.6±8.1, 48.1±4.2 pg/mg protein) but not in 4 week diabetic rats. There was no change in plasma endothelin-1 levels in these diabetic rats. The RT-PCR results indicated that preproendothelin mRNA levels in the mesenteric artery (0.38±0.02 vs 0.52±0.05 units) and in the thoracic aorta (0.45±0.06 vs 0.62±0.03 units) decreased in 4 week diabetic rats but not in 2 week diabetic rats. A significant increase in K(d) and B(max) of endothelin receptors in the mesenteric artery and in the thoracic aorta was observed in both 2 week (about 70%) and 4 week (80-85%) diabetic rats. Insulin replacement reversed the effects of diabetes on endothelin-1 peptide contents, preproendothelin mRNA levels and the binding activity in the blood vessels. Conclusion: Increased endothelin peptide content with no change in mRNA or decreased mRNA levels with no change in peptide content together with increased receptor binding sites and affinities might imply a decrease in endothelin release and therefore an impaired paracrine effect of endothelin on vascular smooth muscles in these STZ-diabetic rats. | en_US |
dc.language | eng | en_US |
dc.publisher | Oxford University Press. The Journal's web site is located at http://cardiovascres.oxfordjournals.org | en_US |
dc.relation.ispartof | Cardiovascular Research | en_US |
dc.rights | Cardiovascular Research. Copyright © Elsevier BV. | - |
dc.subject | Diabetes | - |
dc.subject | Endothelin | - |
dc.subject | Paracrine | - |
dc.subject | Rat | - |
dc.subject | Receptors | - |
dc.subject | Vascular smooth muscle | - |
dc.subject.mesh | Analysis Of Variance | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Aorta, Thoracic - Chemistry - Metabolism | en_US |
dc.subject.mesh | Blotting, Southern | en_US |
dc.subject.mesh | Diabetes Mellitus, Experimental - Metabolism | en_US |
dc.subject.mesh | Endothelin-1 - Blood - Metabolism | en_US |
dc.subject.mesh | Endothelins - Genetics | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Mesenteric Arteries - Chemistry - Metabolism | en_US |
dc.subject.mesh | Muscle, Smooth, Vascular - Chemistry - Metabolism | en_US |
dc.subject.mesh | Paracrine Communication | en_US |
dc.subject.mesh | Protein Binding | en_US |
dc.subject.mesh | Protein Precursors - Genetics | en_US |
dc.subject.mesh | Rna, Messenger - Analysis | en_US |
dc.subject.mesh | Radioimmunoassay | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.subject.mesh | Receptors, Endothelin - Metabolism | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.title | Impaired paracrine effect of endothelin-1 on vascular smooth muscle in streptozotocin-diabetic: Rats | en_US |
dc.type | Article | en_US |
dc.identifier.email | Tang, F:ftang@hkucc.hku.hk | en_US |
dc.identifier.authority | Tang, F=rp00327 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/S0008-6363(98)00176-X | en_US |
dc.identifier.pmid | 9861308 | - |
dc.identifier.scopus | eid_2-s2.0-0032168269 | en_US |
dc.identifier.hkuros | 36690 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0032168269&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 39 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 651 | en_US |
dc.identifier.epage | 656 | en_US |
dc.identifier.isi | WOS:000075916700017 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Wu, SQ=7407180073 | en_US |
dc.identifier.scopusauthorid | Tang, F=7201979770 | en_US |
dc.identifier.issnl | 0008-6363 | - |