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Article: Impaired paracrine effect of endothelin-1 on vascular smooth muscle in streptozotocin-diabetic: Rats

TitleImpaired paracrine effect of endothelin-1 on vascular smooth muscle in streptozotocin-diabetic: Rats
Authors
Issue Date1998
PublisherOxford University Press. The Journal's web site is located at http://cardiovascres.oxfordjournals.org
Citation
Cardiovascular Research, 1998, v. 39 n. 3, p. 651-656 How to Cite?
AbstractObjective: This study was to examine the effect of streptozotocin- induced diabetes on endothelin-1 and its receptors in the mesenteric artery and in the thoracic aorta. Methods: Diabetes was induced in SD rats by streptozotocin. Insulin was given subcutaneously. Endothelin-1 levels in the plasma, thoracic aorta and mesenteric artery were measured using radioimmunoassay. The B(max) and K(d) values of endothelin-1 receptors in the mesenteric artery and in the thoracic aorta were analyzed using Scatchard plot analysis. Preproendothelin mRNA levels were examined using RT-PCR. Results: Endothelin-1 levels in the mesenteric artery (83.6±6.9 pg/mg protein) and in the thoracic aorta (73.9±8.2 pg/mg protein) increased in 2 week diabetic rats compared with both control (51.8±5.3, 46.3±5.9 pg/mg protein) and insulin treated rats (65.6±8.1, 48.1±4.2 pg/mg protein) but not in 4 week diabetic rats. There was no change in plasma endothelin-1 levels in these diabetic rats. The RT-PCR results indicated that preproendothelin mRNA levels in the mesenteric artery (0.38±0.02 vs 0.52±0.05 units) and in the thoracic aorta (0.45±0.06 vs 0.62±0.03 units) decreased in 4 week diabetic rats but not in 2 week diabetic rats. A significant increase in K(d) and B(max) of endothelin receptors in the mesenteric artery and in the thoracic aorta was observed in both 2 week (about 70%) and 4 week (80-85%) diabetic rats. Insulin replacement reversed the effects of diabetes on endothelin-1 peptide contents, preproendothelin mRNA levels and the binding activity in the blood vessels. Conclusion: Increased endothelin peptide content with no change in mRNA or decreased mRNA levels with no change in peptide content together with increased receptor binding sites and affinities might imply a decrease in endothelin release and therefore an impaired paracrine effect of endothelin on vascular smooth muscles in these STZ-diabetic rats.
Persistent Identifierhttp://hdl.handle.net/10722/171650
ISSN
2015 Impact Factor: 5.465
2015 SCImago Journal Rankings: 2.897
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWu, SQen_US
dc.contributor.authorTang, Fen_US
dc.date.accessioned2012-10-30T06:16:09Z-
dc.date.available2012-10-30T06:16:09Z-
dc.date.issued1998en_US
dc.identifier.citationCardiovascular Research, 1998, v. 39 n. 3, p. 651-656en_US
dc.identifier.issn0008-6363en_US
dc.identifier.urihttp://hdl.handle.net/10722/171650-
dc.description.abstractObjective: This study was to examine the effect of streptozotocin- induced diabetes on endothelin-1 and its receptors in the mesenteric artery and in the thoracic aorta. Methods: Diabetes was induced in SD rats by streptozotocin. Insulin was given subcutaneously. Endothelin-1 levels in the plasma, thoracic aorta and mesenteric artery were measured using radioimmunoassay. The B(max) and K(d) values of endothelin-1 receptors in the mesenteric artery and in the thoracic aorta were analyzed using Scatchard plot analysis. Preproendothelin mRNA levels were examined using RT-PCR. Results: Endothelin-1 levels in the mesenteric artery (83.6±6.9 pg/mg protein) and in the thoracic aorta (73.9±8.2 pg/mg protein) increased in 2 week diabetic rats compared with both control (51.8±5.3, 46.3±5.9 pg/mg protein) and insulin treated rats (65.6±8.1, 48.1±4.2 pg/mg protein) but not in 4 week diabetic rats. There was no change in plasma endothelin-1 levels in these diabetic rats. The RT-PCR results indicated that preproendothelin mRNA levels in the mesenteric artery (0.38±0.02 vs 0.52±0.05 units) and in the thoracic aorta (0.45±0.06 vs 0.62±0.03 units) decreased in 4 week diabetic rats but not in 2 week diabetic rats. A significant increase in K(d) and B(max) of endothelin receptors in the mesenteric artery and in the thoracic aorta was observed in both 2 week (about 70%) and 4 week (80-85%) diabetic rats. Insulin replacement reversed the effects of diabetes on endothelin-1 peptide contents, preproendothelin mRNA levels and the binding activity in the blood vessels. Conclusion: Increased endothelin peptide content with no change in mRNA or decreased mRNA levels with no change in peptide content together with increased receptor binding sites and affinities might imply a decrease in endothelin release and therefore an impaired paracrine effect of endothelin on vascular smooth muscles in these STZ-diabetic rats.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://cardiovascres.oxfordjournals.orgen_US
dc.relation.ispartofCardiovascular Researchen_US
dc.rightsCardiovascular Research. Copyright © Elsevier BV.-
dc.subject.meshAnalysis Of Varianceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAorta, Thoracic - Chemistry - Metabolismen_US
dc.subject.meshBlotting, Southernen_US
dc.subject.meshDiabetes Mellitus, Experimental - Metabolismen_US
dc.subject.meshEndothelin-1 - Blood - Metabolismen_US
dc.subject.meshEndothelins - Geneticsen_US
dc.subject.meshMaleen_US
dc.subject.meshMesenteric Arteries - Chemistry - Metabolismen_US
dc.subject.meshMuscle, Smooth, Vascular - Chemistry - Metabolismen_US
dc.subject.meshParacrine Communicationen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshProtein Precursors - Geneticsen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshRadioimmunoassayen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptors, Endothelin - Metabolismen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.titleImpaired paracrine effect of endothelin-1 on vascular smooth muscle in streptozotocin-diabetic: Ratsen_US
dc.typeArticleen_US
dc.identifier.emailTang, F:ftang@hkucc.hku.hken_US
dc.identifier.authorityTang, F=rp00327en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0008-6363(98)00176-Xen_US
dc.identifier.pmid9861308-
dc.identifier.scopuseid_2-s2.0-0032168269en_US
dc.identifier.hkuros36690-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032168269&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume39en_US
dc.identifier.issue3en_US
dc.identifier.spage651en_US
dc.identifier.epage656en_US
dc.identifier.isiWOS:000075916700017-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridWu, SQ=7407180073en_US
dc.identifier.scopusauthoridTang, F=7201979770en_US

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