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- Scopus: eid_2-s2.0-0028941491
- PMID: 7900778
- WOS: WOS:A1995QM03200027
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Article: Metabolism of cyclic ADP-ribose in opossum kidney renal epithelial cells
Title | Metabolism of cyclic ADP-ribose in opossum kidney renal epithelial cells |
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Authors | |
Keywords | β-nicotinamide adenine dinucleotide adenosine 5'-diphosphate-ribose cyclic adenosine 5'-diphosphate-ribose intracellular calcium LLC-PK1 cells renal epithelia sodium-inorganic phosphate symport |
Issue Date | 1995 |
Publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/ |
Citation | American Journal Of Physiology - Cell Physiology, 1995, v. 268 n. 3 37-3, p. C741-C746 How to Cite? |
Abstract | We have previously shown that NAD+ inhibits renal Na+-P(i) symport; however, the biochemical mechanism of NAD+ in this action is not clarified. We now propose that NAD+ acts indirectly by first being converted to cyclic ADP-ribose (cADPR), a potent stimulator of intracellular Ca2+ mobilization. In permeabilized opossum kidney (OK) cells, a cell line often employed as a model for study of proximal tubular epithelial transport, cADPR is synthesized from β-NAD+ in a substrate concentration (0.01-1 mM) and time- dependent manner. That cADPR was generated from β-NAD+ by OK cells was verified by coelution with authentic cADPR on anion exchange high-performance liquid chromatography and by homologous desensitization of the Ca2+ release bioassay to authentic cADPR. cADPR synthesized by permeabilized OK cells was not influenced by the addition of parathyroid hormone. The OK cell also contains the enzyme activity necessary to catalyze catabolism of cADPR. Identification of these two key enzyme activities of cADPR metabolism in OK cells is consistent with a possible role of cADPR in regulation of the Na+- P(i) symporter by NAD+ in response to metabolic stimuli. |
Persistent Identifier | http://hdl.handle.net/10722/171616 |
ISSN | 2023 Impact Factor: 5.0 2023 SCImago Journal Rankings: 1.711 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Beers, KW | en_US |
dc.contributor.author | Chini, EN | en_US |
dc.contributor.author | Hon Cheung Lee | en_US |
dc.contributor.author | Dousa, TP | en_US |
dc.date.accessioned | 2012-10-30T06:15:59Z | - |
dc.date.available | 2012-10-30T06:15:59Z | - |
dc.date.issued | 1995 | en_US |
dc.identifier.citation | American Journal Of Physiology - Cell Physiology, 1995, v. 268 n. 3 37-3, p. C741-C746 | en_US |
dc.identifier.issn | 0363-6143 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171616 | - |
dc.description.abstract | We have previously shown that NAD+ inhibits renal Na+-P(i) symport; however, the biochemical mechanism of NAD+ in this action is not clarified. We now propose that NAD+ acts indirectly by first being converted to cyclic ADP-ribose (cADPR), a potent stimulator of intracellular Ca2+ mobilization. In permeabilized opossum kidney (OK) cells, a cell line often employed as a model for study of proximal tubular epithelial transport, cADPR is synthesized from β-NAD+ in a substrate concentration (0.01-1 mM) and time- dependent manner. That cADPR was generated from β-NAD+ by OK cells was verified by coelution with authentic cADPR on anion exchange high-performance liquid chromatography and by homologous desensitization of the Ca2+ release bioassay to authentic cADPR. cADPR synthesized by permeabilized OK cells was not influenced by the addition of parathyroid hormone. The OK cell also contains the enzyme activity necessary to catalyze catabolism of cADPR. Identification of these two key enzyme activities of cADPR metabolism in OK cells is consistent with a possible role of cADPR in regulation of the Na+- P(i) symporter by NAD+ in response to metabolic stimuli. | en_US |
dc.language | eng | en_US |
dc.publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/ | en_US |
dc.relation.ispartof | American Journal of Physiology - Cell Physiology | en_US |
dc.subject | β-nicotinamide adenine dinucleotide | - |
dc.subject | adenosine 5'-diphosphate-ribose | - |
dc.subject | cyclic adenosine 5'-diphosphate-ribose | - |
dc.subject | intracellular calcium | - |
dc.subject | LLC-PK1 cells | - |
dc.subject | renal epithelia | - |
dc.subject | sodium-inorganic phosphate symport | - |
dc.subject.mesh | Adp-Ribosyl Cyclase | en_US |
dc.subject.mesh | Adenosine Diphosphate Ribose - Analogs & Derivatives - Metabolism - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antigens, Cd | en_US |
dc.subject.mesh | Antigens, Cd38 | en_US |
dc.subject.mesh | Antigens, Differentiation - Metabolism - Pharmacology | en_US |
dc.subject.mesh | Biological Assay | en_US |
dc.subject.mesh | Calcium - Metabolism | en_US |
dc.subject.mesh | Carrier Proteins - Metabolism | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Cell Membrane Permeability | en_US |
dc.subject.mesh | Cyclic Adp-Ribose | en_US |
dc.subject.mesh | Epithelium - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Kidney - Drug Effects - Metabolism | en_US |
dc.subject.mesh | N-Glycosyl Hydrolases - Metabolism - Pharmacology | en_US |
dc.subject.mesh | Nad - Metabolism - Pharmacology | en_US |
dc.subject.mesh | Niacinamide - Pharmacology | en_US |
dc.subject.mesh | Opossums | en_US |
dc.subject.mesh | Ovum - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Parathyroid Hormone - Pharmacology | en_US |
dc.subject.mesh | Sea Urchins | en_US |
dc.subject.mesh | Sodium-Phosphate Cotransporter Proteins | en_US |
dc.subject.mesh | Symporters | en_US |
dc.title | Metabolism of cyclic ADP-ribose in opossum kidney renal epithelial cells | en_US |
dc.type | Article | en_US |
dc.identifier.email | Hon Cheung Lee:leehc@hku.hk | en_US |
dc.identifier.authority | Hon Cheung Lee=rp00545 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 7900778 | - |
dc.identifier.scopus | eid_2-s2.0-0028941491 | en_US |
dc.identifier.volume | 268 | en_US |
dc.identifier.issue | 3 37-3 | en_US |
dc.identifier.spage | C741 | en_US |
dc.identifier.epage | C746 | en_US |
dc.identifier.isi | WOS:A1995QM03200027 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Beers, KW=8844391500 | en_US |
dc.identifier.scopusauthorid | Chini, EN=7003775869 | en_US |
dc.identifier.scopusauthorid | Hon Cheung Lee=26642959100 | en_US |
dc.identifier.scopusauthorid | Dousa, TP=35510540700 | en_US |
dc.identifier.issnl | 0363-6143 | - |