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Article: Metabolism of cyclic ADP-ribose in opossum kidney renal epithelial cells

TitleMetabolism of cyclic ADP-ribose in opossum kidney renal epithelial cells
Authors
Issue Date1995
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/
Citation
American Journal Of Physiology - Cell Physiology, 1995, v. 268 n. 3 37-3, p. C741-C746 How to Cite?
AbstractWe have previously shown that NAD+ inhibits renal Na+-P(i) symport; however, the biochemical mechanism of NAD+ in this action is not clarified. We now propose that NAD+ acts indirectly by first being converted to cyclic ADP-ribose (cADPR), a potent stimulator of intracellular Ca2+ mobilization. In permeabilized opossum kidney (OK) cells, a cell line often employed as a model for study of proximal tubular epithelial transport, cADPR is synthesized from β-NAD+ in a substrate concentration (0.01-1 mM) and time- dependent manner. That cADPR was generated from β-NAD+ by OK cells was verified by coelution with authentic cADPR on anion exchange high-performance liquid chromatography and by homologous desensitization of the Ca2+ release bioassay to authentic cADPR. cADPR synthesized by permeabilized OK cells was not influenced by the addition of parathyroid hormone. The OK cell also contains the enzyme activity necessary to catalyze catabolism of cADPR. Identification of these two key enzyme activities of cADPR metabolism in OK cells is consistent with a possible role of cADPR in regulation of the Na+- P(i) symporter by NAD+ in response to metabolic stimuli.
Persistent Identifierhttp://hdl.handle.net/10722/171616
ISSN
2015 Impact Factor: 3.395
2015 SCImago Journal Rankings: 1.893
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBeers, KWen_US
dc.contributor.authorChini, ENen_US
dc.contributor.authorHon Cheung Leeen_US
dc.contributor.authorDousa, TPen_US
dc.date.accessioned2012-10-30T06:15:59Z-
dc.date.available2012-10-30T06:15:59Z-
dc.date.issued1995en_US
dc.identifier.citationAmerican Journal Of Physiology - Cell Physiology, 1995, v. 268 n. 3 37-3, p. C741-C746en_US
dc.identifier.issn0363-6143en_US
dc.identifier.urihttp://hdl.handle.net/10722/171616-
dc.description.abstractWe have previously shown that NAD+ inhibits renal Na+-P(i) symport; however, the biochemical mechanism of NAD+ in this action is not clarified. We now propose that NAD+ acts indirectly by first being converted to cyclic ADP-ribose (cADPR), a potent stimulator of intracellular Ca2+ mobilization. In permeabilized opossum kidney (OK) cells, a cell line often employed as a model for study of proximal tubular epithelial transport, cADPR is synthesized from β-NAD+ in a substrate concentration (0.01-1 mM) and time- dependent manner. That cADPR was generated from β-NAD+ by OK cells was verified by coelution with authentic cADPR on anion exchange high-performance liquid chromatography and by homologous desensitization of the Ca2+ release bioassay to authentic cADPR. cADPR synthesized by permeabilized OK cells was not influenced by the addition of parathyroid hormone. The OK cell also contains the enzyme activity necessary to catalyze catabolism of cADPR. Identification of these two key enzyme activities of cADPR metabolism in OK cells is consistent with a possible role of cADPR in regulation of the Na+- P(i) symporter by NAD+ in response to metabolic stimuli.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Cell Physiologyen_US
dc.subject.meshAdp-Ribosyl Cyclaseen_US
dc.subject.meshAdenosine Diphosphate Ribose - Analogs & Derivatives - Metabolism - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Cden_US
dc.subject.meshAntigens, Cd38en_US
dc.subject.meshAntigens, Differentiation - Metabolism - Pharmacologyen_US
dc.subject.meshBiological Assayen_US
dc.subject.meshCalcium - Metabolismen_US
dc.subject.meshCarrier Proteins - Metabolismen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Membrane Permeabilityen_US
dc.subject.meshCyclic Adp-Riboseen_US
dc.subject.meshEpithelium - Drug Effects - Metabolismen_US
dc.subject.meshKidney - Drug Effects - Metabolismen_US
dc.subject.meshN-Glycosyl Hydrolases - Metabolism - Pharmacologyen_US
dc.subject.meshNad - Metabolism - Pharmacologyen_US
dc.subject.meshNiacinamide - Pharmacologyen_US
dc.subject.meshOpossumsen_US
dc.subject.meshOvum - Drug Effects - Metabolismen_US
dc.subject.meshParathyroid Hormone - Pharmacologyen_US
dc.subject.meshSea Urchinsen_US
dc.subject.meshSodium-Phosphate Cotransporter Proteinsen_US
dc.subject.meshSymportersen_US
dc.titleMetabolism of cyclic ADP-ribose in opossum kidney renal epithelial cellsen_US
dc.typeArticleen_US
dc.identifier.emailHon Cheung Lee:leehc@hku.hken_US
dc.identifier.authorityHon Cheung Lee=rp00545en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7900778-
dc.identifier.scopuseid_2-s2.0-0028941491en_US
dc.identifier.volume268en_US
dc.identifier.issue3 37-3en_US
dc.identifier.spageC741en_US
dc.identifier.epageC746en_US
dc.identifier.isiWOS:A1995QM03200027-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridBeers, KW=8844391500en_US
dc.identifier.scopusauthoridChini, EN=7003775869en_US
dc.identifier.scopusauthoridHon Cheung Lee=26642959100en_US
dc.identifier.scopusauthoridDousa, TP=35510540700en_US

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