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Article: NADPH-diaphorase histochemistry identifies isolated endothelial cells at sites of traumatic injury in the adult rat brain

TitleNADPH-diaphorase histochemistry identifies isolated endothelial cells at sites of traumatic injury in the adult rat brain
Authors
Issue Date1993
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
Citation
Neuroscience, 1993, v. 53 n. 3, p. 613-624 How to Cite?
AbstractIn addition to labelling endothelium, some ependymal cells (including tanycytes), and a subpopulation of neurons, nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of stab lesion sites in the neocortex revealed a large population of cells concentrated within several hundred micrometres of the lesion site. To determine the identity of these cells, NADPH-diaphorase reactivity was compared to binding with either the I-B 4 isolectin from Bandeiraea simplicifolia (which has previously been shown to identify endothelial cells and activated mononuclear phagocytes), or a monoclonal antibody (OX-42) that recognizes activated mononuclear phagocytes. Many I-B 4 lectin-labelled cells were also NADPH-diaphorase reactive, and other I-B 4 lectin-labelled cells were also OX-42 immunoreactive, but co-existence of OX-42 immunoreactivity and NADPH-diaphorase reactivity was not observed. Only a small minority of NADPH-diaphorase-reactive cells did not exhibit I-B 4 lectin binding. In contrast to the simple somatic morphology of the majority of NADPH-diaphorase-reactive cells, the I-B 4 lectin-negative cells had a ramified appearance, and while readily observed at two days postlesion, they were only rarely seen at three days postlesion. Primary cultures of bovine aortic endothelial cells also exhibited NADPH-diaphorase reactivity which occupied most of the cytoplasm in a filamentous web pattern. Endothelial cells possess a constitutive form of nitric oxide synthase which, as demonstrated in NADPH-diaphorase-reactive neurons, may be the basis of their NADPH-diaphorase reactivity. These findings indicate that NADPH-diaphorase-reactive cells observed at lesion sites are probably angiogenic endothelial cells not associated with extant blood vessels. Thus, NADPH-diaphorase histochemistry offers an effective method of visualizing neovascularization in the brain and other tissues.
Persistent Identifierhttp://hdl.handle.net/10722/171595
ISSN
2015 Impact Factor: 3.231
2015 SCImago Journal Rankings: 1.768
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKitchener, PDen_US
dc.contributor.authorBourreau, JPen_US
dc.contributor.authorDiamond, Jen_US
dc.date.accessioned2012-10-30T06:15:53Z-
dc.date.available2012-10-30T06:15:53Z-
dc.date.issued1993en_US
dc.identifier.citationNeuroscience, 1993, v. 53 n. 3, p. 613-624en_US
dc.identifier.issn0306-4522en_US
dc.identifier.urihttp://hdl.handle.net/10722/171595-
dc.description.abstractIn addition to labelling endothelium, some ependymal cells (including tanycytes), and a subpopulation of neurons, nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of stab lesion sites in the neocortex revealed a large population of cells concentrated within several hundred micrometres of the lesion site. To determine the identity of these cells, NADPH-diaphorase reactivity was compared to binding with either the I-B 4 isolectin from Bandeiraea simplicifolia (which has previously been shown to identify endothelial cells and activated mononuclear phagocytes), or a monoclonal antibody (OX-42) that recognizes activated mononuclear phagocytes. Many I-B 4 lectin-labelled cells were also NADPH-diaphorase reactive, and other I-B 4 lectin-labelled cells were also OX-42 immunoreactive, but co-existence of OX-42 immunoreactivity and NADPH-diaphorase reactivity was not observed. Only a small minority of NADPH-diaphorase-reactive cells did not exhibit I-B 4 lectin binding. In contrast to the simple somatic morphology of the majority of NADPH-diaphorase-reactive cells, the I-B 4 lectin-negative cells had a ramified appearance, and while readily observed at two days postlesion, they were only rarely seen at three days postlesion. Primary cultures of bovine aortic endothelial cells also exhibited NADPH-diaphorase reactivity which occupied most of the cytoplasm in a filamentous web pattern. Endothelial cells possess a constitutive form of nitric oxide synthase which, as demonstrated in NADPH-diaphorase-reactive neurons, may be the basis of their NADPH-diaphorase reactivity. These findings indicate that NADPH-diaphorase-reactive cells observed at lesion sites are probably angiogenic endothelial cells not associated with extant blood vessels. Thus, NADPH-diaphorase histochemistry offers an effective method of visualizing neovascularization in the brain and other tissues.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscienceen_US
dc.relation.ispartofNeuroscienceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal - Immunologyen_US
dc.subject.meshBrain - Pathologyen_US
dc.subject.meshBrain Injuries - Pathologyen_US
dc.subject.meshCerebral Cortex - Pathologyen_US
dc.subject.meshEndothelium - Cytologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHistocytochemistryen_US
dc.subject.meshLectinsen_US
dc.subject.meshMacrophages - Ultrastructureen_US
dc.subject.meshNadph Dehydrogenase - Analysisen_US
dc.subject.meshNeuroglia - Ultrastructureen_US
dc.subject.meshPlant Lectinsen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.titleNADPH-diaphorase histochemistry identifies isolated endothelial cells at sites of traumatic injury in the adult rat brainen_US
dc.typeArticleen_US
dc.identifier.emailBourreau, JP:bourreau@hkucc.hku.hken_US
dc.identifier.authorityBourreau, JP=rp00389en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0306-4522(93)90610-Ren_US
dc.identifier.pmid8487946-
dc.identifier.scopuseid_2-s2.0-0027526534en_US
dc.identifier.volume53en_US
dc.identifier.issue3en_US
dc.identifier.spage613en_US
dc.identifier.epage624en_US
dc.identifier.isiWOS:A1993KW54700002-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridKitchener, PD=35549211200en_US
dc.identifier.scopusauthoridBourreau, JP=7003927886en_US
dc.identifier.scopusauthoridDiamond, J=36489314700en_US

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