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- Publisher Website: 10.1016/0306-4522(93)90610-R
- Scopus: eid_2-s2.0-0027526534
- PMID: 8487946
- WOS: WOS:A1993KW54700002
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Article: NADPH-diaphorase histochemistry identifies isolated endothelial cells at sites of traumatic injury in the adult rat brain
Title | NADPH-diaphorase histochemistry identifies isolated endothelial cells at sites of traumatic injury in the adult rat brain |
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Authors | |
Issue Date | 1993 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience |
Citation | Neuroscience, 1993, v. 53 n. 3, p. 613-624 How to Cite? |
Abstract | In addition to labelling endothelium, some ependymal cells (including tanycytes), and a subpopulation of neurons, nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of stab lesion sites in the neocortex revealed a large population of cells concentrated within several hundred micrometres of the lesion site. To determine the identity of these cells, NADPH-diaphorase reactivity was compared to binding with either the I-B 4 isolectin from Bandeiraea simplicifolia (which has previously been shown to identify endothelial cells and activated mononuclear phagocytes), or a monoclonal antibody (OX-42) that recognizes activated mononuclear phagocytes. Many I-B 4 lectin-labelled cells were also NADPH-diaphorase reactive, and other I-B 4 lectin-labelled cells were also OX-42 immunoreactive, but co-existence of OX-42 immunoreactivity and NADPH-diaphorase reactivity was not observed. Only a small minority of NADPH-diaphorase-reactive cells did not exhibit I-B 4 lectin binding. In contrast to the simple somatic morphology of the majority of NADPH-diaphorase-reactive cells, the I-B 4 lectin-negative cells had a ramified appearance, and while readily observed at two days postlesion, they were only rarely seen at three days postlesion. Primary cultures of bovine aortic endothelial cells also exhibited NADPH-diaphorase reactivity which occupied most of the cytoplasm in a filamentous web pattern. Endothelial cells possess a constitutive form of nitric oxide synthase which, as demonstrated in NADPH-diaphorase-reactive neurons, may be the basis of their NADPH-diaphorase reactivity. These findings indicate that NADPH-diaphorase-reactive cells observed at lesion sites are probably angiogenic endothelial cells not associated with extant blood vessels. Thus, NADPH-diaphorase histochemistry offers an effective method of visualizing neovascularization in the brain and other tissues. |
Persistent Identifier | http://hdl.handle.net/10722/171595 |
ISSN | 2023 Impact Factor: 2.9 2023 SCImago Journal Rankings: 0.903 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Kitchener, PD | en_US |
dc.contributor.author | Bourreau, JP | en_US |
dc.contributor.author | Diamond, J | en_US |
dc.date.accessioned | 2012-10-30T06:15:53Z | - |
dc.date.available | 2012-10-30T06:15:53Z | - |
dc.date.issued | 1993 | en_US |
dc.identifier.citation | Neuroscience, 1993, v. 53 n. 3, p. 613-624 | en_US |
dc.identifier.issn | 0306-4522 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171595 | - |
dc.description.abstract | In addition to labelling endothelium, some ependymal cells (including tanycytes), and a subpopulation of neurons, nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of stab lesion sites in the neocortex revealed a large population of cells concentrated within several hundred micrometres of the lesion site. To determine the identity of these cells, NADPH-diaphorase reactivity was compared to binding with either the I-B 4 isolectin from Bandeiraea simplicifolia (which has previously been shown to identify endothelial cells and activated mononuclear phagocytes), or a monoclonal antibody (OX-42) that recognizes activated mononuclear phagocytes. Many I-B 4 lectin-labelled cells were also NADPH-diaphorase reactive, and other I-B 4 lectin-labelled cells were also OX-42 immunoreactive, but co-existence of OX-42 immunoreactivity and NADPH-diaphorase reactivity was not observed. Only a small minority of NADPH-diaphorase-reactive cells did not exhibit I-B 4 lectin binding. In contrast to the simple somatic morphology of the majority of NADPH-diaphorase-reactive cells, the I-B 4 lectin-negative cells had a ramified appearance, and while readily observed at two days postlesion, they were only rarely seen at three days postlesion. Primary cultures of bovine aortic endothelial cells also exhibited NADPH-diaphorase reactivity which occupied most of the cytoplasm in a filamentous web pattern. Endothelial cells possess a constitutive form of nitric oxide synthase which, as demonstrated in NADPH-diaphorase-reactive neurons, may be the basis of their NADPH-diaphorase reactivity. These findings indicate that NADPH-diaphorase-reactive cells observed at lesion sites are probably angiogenic endothelial cells not associated with extant blood vessels. Thus, NADPH-diaphorase histochemistry offers an effective method of visualizing neovascularization in the brain and other tissues. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience | en_US |
dc.relation.ispartof | Neuroscience | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibodies, Monoclonal - Immunology | en_US |
dc.subject.mesh | Brain - Pathology | en_US |
dc.subject.mesh | Brain Injuries - Pathology | en_US |
dc.subject.mesh | Cerebral Cortex - Pathology | en_US |
dc.subject.mesh | Endothelium - Cytology | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Histocytochemistry | en_US |
dc.subject.mesh | Lectins | en_US |
dc.subject.mesh | Macrophages - Ultrastructure | en_US |
dc.subject.mesh | Nadph Dehydrogenase - Analysis | en_US |
dc.subject.mesh | Neuroglia - Ultrastructure | en_US |
dc.subject.mesh | Plant Lectins | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Wistar | en_US |
dc.title | NADPH-diaphorase histochemistry identifies isolated endothelial cells at sites of traumatic injury in the adult rat brain | en_US |
dc.type | Article | en_US |
dc.identifier.email | Bourreau, JP:bourreau@hkucc.hku.hk | en_US |
dc.identifier.authority | Bourreau, JP=rp00389 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/0306-4522(93)90610-R | en_US |
dc.identifier.pmid | 8487946 | - |
dc.identifier.scopus | eid_2-s2.0-0027526534 | en_US |
dc.identifier.volume | 53 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 613 | en_US |
dc.identifier.epage | 624 | en_US |
dc.identifier.isi | WOS:A1993KW54700002 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Kitchener, PD=35549211200 | en_US |
dc.identifier.scopusauthorid | Bourreau, JP=7003927886 | en_US |
dc.identifier.scopusauthorid | Diamond, J=36489314700 | en_US |
dc.identifier.issnl | 0306-4522 | - |