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- Scopus: eid_2-s2.0-0027301455
- PMID: 7687823
- WOS: WOS:A1993LP43100005
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Article: Distinct pathways to refill ACh-sensitive internal Ca2+ stores in canine airway smooth muscle
Title | Distinct pathways to refill ACh-sensitive internal Ca2+ stores in canine airway smooth muscle |
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Authors | |
Keywords | calcium channels calcium stores cyclopiazonic acid |
Issue Date | 1993 |
Publisher | American Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/ |
Citation | American Journal Of Physiology - Cell Physiology, 1993, v. 265 n. 1 34-1, p. C28-C35 How to Cite? |
Abstract | The ability of extracellular Ca2+ to refill internal Ca2+ stores of canine tracheal smooth muscle after a prior depletion by acetylcholine (ACh) was assessed using a novel sarcoplasmic reticulum (SR) Ca2+ pump inhibitor, cyclopiazonic acid (CPA). The transient contraction induced by ACh in a medium free of Ca2+ was used as an index for the content of agonist- sensitive intracellular Ca2+ stores. CPA inhibited in a concentration- dependent manner the refilling of the stores occurring during high KCl stimulation, and this inhibitory effect was independent of the external Ca2+ concentration. On the other hand, CPA was less effective in inhibiting the refilling occurring during prolonged ACh stimulation, especially when external Ca2+ concentration was raised. At 5.0 mM external Ca2+ or when 0.1 μM BAY K 8644 was present in the medium, CPA was ineffective in inhibiting the refilling occurring during prolonged ACh stimulation. The maximum ACh-induced contraction in Ca2+-containing medium was independent of the extent of internal store Ca2+ load in the absence of L-type Ca2+ channel blocker but was highly dependent on the extent of internal Ca2+ load in the presence of the Ca2+ channel blocker. Hyperpolarization of the plasma membrane with the K+ channel opener cromakalim reduced the amplitude of ACh tonic contraction. Subsequent addition of nifedipine further reduced ACh tonic contraction. It is concluded that two different pathways for external Ca2+ are used to refill ACh-sensitive internal stores. One involves active Ca2+ uptake via a CPA-sensitive Ca2+ pump, and the other involves a CPA-insensitive pathway whose nature remains to be determined. |
Persistent Identifier | http://hdl.handle.net/10722/171581 |
ISSN | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Bourreau, JP | en_US |
dc.contributor.author | Kwan, CY | en_US |
dc.contributor.author | Daniel, EE | en_US |
dc.date.accessioned | 2012-10-30T06:15:48Z | - |
dc.date.available | 2012-10-30T06:15:48Z | - |
dc.date.issued | 1993 | en_US |
dc.identifier.citation | American Journal Of Physiology - Cell Physiology, 1993, v. 265 n. 1 34-1, p. C28-C35 | en_US |
dc.identifier.issn | 0002-9513 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171581 | - |
dc.description.abstract | The ability of extracellular Ca2+ to refill internal Ca2+ stores of canine tracheal smooth muscle after a prior depletion by acetylcholine (ACh) was assessed using a novel sarcoplasmic reticulum (SR) Ca2+ pump inhibitor, cyclopiazonic acid (CPA). The transient contraction induced by ACh in a medium free of Ca2+ was used as an index for the content of agonist- sensitive intracellular Ca2+ stores. CPA inhibited in a concentration- dependent manner the refilling of the stores occurring during high KCl stimulation, and this inhibitory effect was independent of the external Ca2+ concentration. On the other hand, CPA was less effective in inhibiting the refilling occurring during prolonged ACh stimulation, especially when external Ca2+ concentration was raised. At 5.0 mM external Ca2+ or when 0.1 μM BAY K 8644 was present in the medium, CPA was ineffective in inhibiting the refilling occurring during prolonged ACh stimulation. The maximum ACh-induced contraction in Ca2+-containing medium was independent of the extent of internal store Ca2+ load in the absence of L-type Ca2+ channel blocker but was highly dependent on the extent of internal Ca2+ load in the presence of the Ca2+ channel blocker. Hyperpolarization of the plasma membrane with the K+ channel opener cromakalim reduced the amplitude of ACh tonic contraction. Subsequent addition of nifedipine further reduced ACh tonic contraction. It is concluded that two different pathways for external Ca2+ are used to refill ACh-sensitive internal stores. One involves active Ca2+ uptake via a CPA-sensitive Ca2+ pump, and the other involves a CPA-insensitive pathway whose nature remains to be determined. | en_US |
dc.language | eng | en_US |
dc.publisher | American Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/ | en_US |
dc.relation.ispartof | American Journal of Physiology - Cell Physiology | en_US |
dc.subject | calcium channels | - |
dc.subject | calcium stores | - |
dc.subject | cyclopiazonic acid | - |
dc.subject.mesh | 3-Pyridinecarboxylic Acid, 1,4-Dihydro-2,6-Dimethyl-5-Nitro-4-(2-(Trifluoromethyl)Phenyl)-, Methyl Ester - Pharmacology | en_US |
dc.subject.mesh | Acetylcholine - Pharmacology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Benzopyrans - Pharmacology | en_US |
dc.subject.mesh | Calcium - Pharmacology | en_US |
dc.subject.mesh | Cromakalim | en_US |
dc.subject.mesh | Dogs | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Indoles - Pharmacology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Muscle Contraction - Drug Effects | en_US |
dc.subject.mesh | Muscle, Smooth - Metabolism | en_US |
dc.subject.mesh | Nifedipine - Pharmacology | en_US |
dc.subject.mesh | Pyrroles - Pharmacology | en_US |
dc.subject.mesh | Trachea - Metabolism | en_US |
dc.title | Distinct pathways to refill ACh-sensitive internal Ca2+ stores in canine airway smooth muscle | en_US |
dc.type | Article | en_US |
dc.identifier.email | Bourreau, JP:bourreau@hkucc.hku.hk | en_US |
dc.identifier.authority | Bourreau, JP=rp00389 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 7687823 | en_US |
dc.identifier.scopus | eid_2-s2.0-0027301455 | en_US |
dc.identifier.volume | 265 | en_US |
dc.identifier.issue | 1 34-1 | en_US |
dc.identifier.spage | C28 | en_US |
dc.identifier.epage | C35 | en_US |
dc.identifier.isi | WOS:A1993LP43100005 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Bourreau, JP=7003927886 | en_US |
dc.identifier.scopusauthorid | Kwan, CY=7201421224 | en_US |
dc.identifier.scopusauthorid | Daniel, EE=35474017600 | en_US |
dc.identifier.issnl | 0002-9513 | - |