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Article: Envelope protein sequences of dengue virus isolates TH-36 and TH-Sman, and identification of a type-specific genetic marker for dengue and tick-borne flaviviruses

TitleEnvelope protein sequences of dengue virus isolates TH-36 and TH-Sman, and identification of a type-specific genetic marker for dengue and tick-borne flaviviruses
Authors
Issue Date1992
PublisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org
Citation
Journal Of General Virology, 1992, v. 73 n. 1, p. 207-212 How to Cite?
AbstractComplementary DNAs were synthesized from the envelope protein genes of two isolates of dengue virus (TH-36 and TH-Sman, previously suggested as possible dengue virus type 5 and dengue virus type 6 respectively) and amplified by the polymerase chain reaction using sense and antisense primers designed from conserved dengue virus gene sequences. The amplified cDNA clones were sequenced in both directions by double-stranded dideoxynucleotide sequencing. Alignment with published dengue virus sequences enabled us to assign these viruses accurately to classified serotypes, confirming that TH-36 and TH-Sman are strains of dengue virus type 2 and dengue virus type 1 respectively. Amino acid changes between the proteins encoded by these two isolates and strains of their respective serotypes may account for the significant antigenic differences observed during previous serological typing of these viruses. Moreover, sequence alignment of flavivirus envelope proteins revealed a hypervariable region, within which members of the dengue and tick-borne virus antigenic complexes show unique peptide sequences. This type-specific hypervariable domain may be useful as a genetic marker for typing dengue and tick-borne flaviviruses.
Persistent Identifierhttp://hdl.handle.net/10722/171567
ISSN
2015 Impact Factor: 3.192
2015 SCImago Journal Rankings: 1.741
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShiu, SYWen_US
dc.contributor.authorJiang, WRen_US
dc.contributor.authorPorterfield, JSen_US
dc.contributor.authorGould, EAen_US
dc.date.accessioned2012-10-30T06:15:43Z-
dc.date.available2012-10-30T06:15:43Z-
dc.date.issued1992en_US
dc.identifier.citationJournal Of General Virology, 1992, v. 73 n. 1, p. 207-212en_US
dc.identifier.issn0022-1317en_US
dc.identifier.urihttp://hdl.handle.net/10722/171567-
dc.description.abstractComplementary DNAs were synthesized from the envelope protein genes of two isolates of dengue virus (TH-36 and TH-Sman, previously suggested as possible dengue virus type 5 and dengue virus type 6 respectively) and amplified by the polymerase chain reaction using sense and antisense primers designed from conserved dengue virus gene sequences. The amplified cDNA clones were sequenced in both directions by double-stranded dideoxynucleotide sequencing. Alignment with published dengue virus sequences enabled us to assign these viruses accurately to classified serotypes, confirming that TH-36 and TH-Sman are strains of dengue virus type 2 and dengue virus type 1 respectively. Amino acid changes between the proteins encoded by these two isolates and strains of their respective serotypes may account for the significant antigenic differences observed during previous serological typing of these viruses. Moreover, sequence alignment of flavivirus envelope proteins revealed a hypervariable region, within which members of the dengue and tick-borne virus antigenic complexes show unique peptide sequences. This type-specific hypervariable domain may be useful as a genetic marker for typing dengue and tick-borne flaviviruses.en_US
dc.languageengen_US
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.orgen_US
dc.relation.ispartofJournal of General Virologyen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDengue Virus - Geneticsen_US
dc.subject.meshFlavivirus - Geneticsen_US
dc.subject.meshGenetic Markers - Geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshViral Envelope Proteins - Geneticsen_US
dc.titleEnvelope protein sequences of dengue virus isolates TH-36 and TH-Sman, and identification of a type-specific genetic marker for dengue and tick-borne flavivirusesen_US
dc.typeArticleen_US
dc.identifier.emailShiu, SYW:sywshiu@hkucc.hku.hken_US
dc.identifier.authorityShiu, SYW=rp00384en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1099/0022-1317-73-1-207-
dc.identifier.pmid1339466-
dc.identifier.scopuseid_2-s2.0-0026542340en_US
dc.identifier.volume73en_US
dc.identifier.issue1en_US
dc.identifier.spage207en_US
dc.identifier.epage212en_US
dc.identifier.isiWOS:A1992HA16500026-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridShiu, SYW=7005550655en_US
dc.identifier.scopusauthoridJiang, WR=7403697511en_US
dc.identifier.scopusauthoridPorterfield, JS=7005608715en_US
dc.identifier.scopusauthoridGould, EA=7101857623en_US

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