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Article: 17D yellow fever vaccine virus envelope protein expressed by recombinant baculovirus is antigenically indistinguishable from authentic viral protein

Title17D yellow fever vaccine virus envelope protein expressed by recombinant baculovirus is antigenically indistinguishable from authentic viral protein
Authors
Issue Date1991
PublisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org
Citation
Journal Of General Virology, 1991, v. 72 n. 6, p. 1451-1454 How to Cite?
AbstractWe have constructed a recombinant baculovirus containing cloned DNA encoding the membrane and envelope (E) proteins of 17D yellow fever vaccine virus. Spodoptera frugiperda cells infected with this recombinant baculovirus produced a 66K protein which corresponded to the estimated size of the protein encoded by the cloned inserted DNA, and a 54K protein with the same molecular size as that of the authentic 17D yellow fever virus E protein. This recombinant 54K protein was labile, producing E protein-specific breakdown products (45K to 36K). Indirect immunofluorescence, using a panel of E protein-specific monoclonal antibodies, showed that the recombinant protein was presented both inside as well as on the surface of cells and was antigenically indistinguishable from the E protein of 17D yellow fever vaccine virus.
Persistent Identifierhttp://hdl.handle.net/10722/171554
ISSN
2015 Impact Factor: 3.192
2015 SCImago Journal Rankings: 1.741
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShiu, SYWen_US
dc.contributor.authorMorikawa, Sen_US
dc.contributor.authorBuckley, Aen_US
dc.contributor.authorHiggs, Sen_US
dc.contributor.authorKarunakarannair, Ven_US
dc.contributor.authorBlachere, Cen_US
dc.contributor.authorGould, EAen_US
dc.date.accessioned2012-10-30T06:15:39Z-
dc.date.available2012-10-30T06:15:39Z-
dc.date.issued1991en_US
dc.identifier.citationJournal Of General Virology, 1991, v. 72 n. 6, p. 1451-1454en_US
dc.identifier.issn0022-1317en_US
dc.identifier.urihttp://hdl.handle.net/10722/171554-
dc.description.abstractWe have constructed a recombinant baculovirus containing cloned DNA encoding the membrane and envelope (E) proteins of 17D yellow fever vaccine virus. Spodoptera frugiperda cells infected with this recombinant baculovirus produced a 66K protein which corresponded to the estimated size of the protein encoded by the cloned inserted DNA, and a 54K protein with the same molecular size as that of the authentic 17D yellow fever virus E protein. This recombinant 54K protein was labile, producing E protein-specific breakdown products (45K to 36K). Indirect immunofluorescence, using a panel of E protein-specific monoclonal antibodies, showed that the recombinant protein was presented both inside as well as on the surface of cells and was antigenically indistinguishable from the E protein of 17D yellow fever vaccine virus.en_US
dc.languageengen_US
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.orgen_US
dc.relation.ispartofJournal of General Virologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal - Diagnostic Useen_US
dc.subject.meshBaculoviridae - Geneticsen_US
dc.subject.meshCapsid - Geneticsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna, Viral - Geneticsen_US
dc.subject.meshEpitopes - Analysisen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshGenetic Vectorsen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshMothsen_US
dc.subject.meshRecombinant Proteins - Immunologyen_US
dc.subject.meshRestriction Mappingen_US
dc.subject.meshVaccines, Syntheticen_US
dc.subject.meshViral Core Proteins - Geneticsen_US
dc.subject.meshViral Envelope Proteins - Genetics - Immunologyen_US
dc.subject.meshViral Nonstructural Proteinsen_US
dc.subject.meshViral Structural Proteins - Geneticsen_US
dc.subject.meshViral Vaccinesen_US
dc.subject.meshYellow Fever Virus - Genetics - Immunologyen_US
dc.title17D yellow fever vaccine virus envelope protein expressed by recombinant baculovirus is antigenically indistinguishable from authentic viral proteinen_US
dc.typeArticleen_US
dc.identifier.emailShiu, SYW:sywshiu@hkucc.hku.hken_US
dc.identifier.authorityShiu, SYW=rp00384en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1099/0022-1317-72-6-1451-
dc.identifier.pmid1710651-
dc.identifier.scopuseid_2-s2.0-0025869332en_US
dc.identifier.volume72en_US
dc.identifier.issue6en_US
dc.identifier.spage1451en_US
dc.identifier.epage1454en_US
dc.identifier.isiWOS:A1991FQ45300032-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridShiu, SYW=7005550655en_US
dc.identifier.scopusauthoridMorikawa, S=7102226341en_US
dc.identifier.scopusauthoridBuckley, A=7102458647en_US
dc.identifier.scopusauthoridHiggs, S=34975082700en_US
dc.identifier.scopusauthoridKarunakarannair, V=6504279759en_US
dc.identifier.scopusauthoridBlachere, C=55283749000en_US
dc.identifier.scopusauthoridGould, EA=7101857623en_US

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