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Article: Determination of endogenous levels of cyclic ADP-ribose in rat tissues

TitleDetermination of endogenous levels of cyclic ADP-ribose in rat tissues
Authors
Issue Date1991
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcr
Citation
Biochimica Et Biophysica Acta - Molecular Cell Research, 1991, v. 1094 n. 1, p. 113-120 How to Cite?
AbstractCyclic ADP-ribose (cADPR) is a potent mediator of calcium mobilization in sea urchin eggs. The cADPR synthesizing enzyme is present not only in the eggs but also in various mammalian tissue extracts. The purpose of this study was to ascertain whether cADPR is a naturally occurring nucleotide in mammalian tissues. Rat tissues were frozen and powdered in liquid N2, followed by extraction with perchloric acid at -10°C. [32P]cADPR was prepared and used as a tracer. The acid extracts were chromatographed on a Mono-Q column and cADPR in the fractions were determined by its ability to release Ca2+ from egg homogenates. That the release was mediated by cADPR and not inositol trisphosphate (IP3) in the extracts was shown by the fact that the homogenates, subsequent to Ca2+ release induced by active fractions, were desensitized to authentic cADPR but not to IP3. Furthermore, the Ca2+ release activity was shown to co-elute with [32P]cADPR. The endogenous level of cADPR determined in rat liver is 3.37 ± 0.64 pmol/mg, in heart is 1.04 ± 0.08 pmol/mg and in brain is 2.75 ± 0.35 pmol/mg. These results indicate cADPR is a naturally occurring nucleotide and suggest that it may be a general second messenger for mobilizing intracellular Ca2+.
Persistent Identifierhttp://hdl.handle.net/10722/171551
ISSN
2015 Impact Factor: 5.128
2015 SCImago Journal Rankings: 3.043
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWalseth, TFen_US
dc.contributor.authorAarhus, Ren_US
dc.contributor.authorZeleznikar Jr, RJen_US
dc.contributor.authorLee, HCen_US
dc.date.accessioned2012-10-30T06:15:38Z-
dc.date.available2012-10-30T06:15:38Z-
dc.date.issued1991en_US
dc.identifier.citationBiochimica Et Biophysica Acta - Molecular Cell Research, 1991, v. 1094 n. 1, p. 113-120en_US
dc.identifier.issn0167-4889en_US
dc.identifier.urihttp://hdl.handle.net/10722/171551-
dc.description.abstractCyclic ADP-ribose (cADPR) is a potent mediator of calcium mobilization in sea urchin eggs. The cADPR synthesizing enzyme is present not only in the eggs but also in various mammalian tissue extracts. The purpose of this study was to ascertain whether cADPR is a naturally occurring nucleotide in mammalian tissues. Rat tissues were frozen and powdered in liquid N2, followed by extraction with perchloric acid at -10°C. [32P]cADPR was prepared and used as a tracer. The acid extracts were chromatographed on a Mono-Q column and cADPR in the fractions were determined by its ability to release Ca2+ from egg homogenates. That the release was mediated by cADPR and not inositol trisphosphate (IP3) in the extracts was shown by the fact that the homogenates, subsequent to Ca2+ release induced by active fractions, were desensitized to authentic cADPR but not to IP3. Furthermore, the Ca2+ release activity was shown to co-elute with [32P]cADPR. The endogenous level of cADPR determined in rat liver is 3.37 ± 0.64 pmol/mg, in heart is 1.04 ± 0.08 pmol/mg and in brain is 2.75 ± 0.35 pmol/mg. These results indicate cADPR is a naturally occurring nucleotide and suggest that it may be a general second messenger for mobilizing intracellular Ca2+.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcren_US
dc.relation.ispartofBiochimica et Biophysica Acta - Molecular Cell Researchen_US
dc.subject.meshAdenosine Diphosphate Ribose - Isolation & Purification - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnion Exchange Resinsen_US
dc.subject.meshBiological Assayen_US
dc.subject.meshBrain - Metabolismen_US
dc.subject.meshCalcium - Metabolismen_US
dc.subject.meshCell Fractionationen_US
dc.subject.meshCyclic Adp-Riboseen_US
dc.subject.meshDogsen_US
dc.subject.meshLiver - Metabolismen_US
dc.subject.meshMyocardium - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshResins, Syntheticen_US
dc.subject.meshSea Urchinsen_US
dc.titleDetermination of endogenous levels of cyclic ADP-ribose in rat tissuesen_US
dc.typeArticleen_US
dc.identifier.emailLee, HC:leehc@hku.hken_US
dc.identifier.authorityLee, HC=rp00545en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0167-4889(91)90032-Sen_US
dc.identifier.pmid1883849-
dc.identifier.scopuseid_2-s2.0-0025831860en_US
dc.identifier.volume1094en_US
dc.identifier.issue1en_US
dc.identifier.spage113en_US
dc.identifier.epage120en_US
dc.identifier.isiWOS:A1991GD60500014-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridWalseth, TF=7005424273en_US
dc.identifier.scopusauthoridAarhus, R=6701339421en_US
dc.identifier.scopusauthoridZeleznikar Jr, RJ=6603006796en_US
dc.identifier.scopusauthoridLee, HC=26642959100en_US

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